Curated Information
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Home > Curated Information Page > PubMed Id: 16061178
López-Bergami P, et al. (2005) RACK1 mediates activation of JNK by protein kinase C [corrected] Mol Cell 19, 309-20 16061178
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S129-p - JNK2 (mouse)
Modsite: ELDHERMsYLLYQML SwissProt Entrez-Gene
Orthologous residues
JNK2 (human): S129‑p, JNK2 iso2 (human): S129‑p, JNK2 iso3 (human): S129‑p, JNK2 (mouse): S129‑p, JNK2 (rat): S129‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCB iso2 (human)
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

T183-p - JNK2 (mouse)
Modsite: ACTNFMMtPyVVtRY SwissProt Entrez-Gene
Orthologous residues
JNK2 (human): T183‑p, JNK2 iso2 (human): T183‑p, JNK2 iso3 (human): T183‑p, JNK2 (mouse): T183‑p, JNK2 (rat): T183‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody
Relevant cell lines - cell types - tissues:  U2OS (bone cell) [GR (human)]
Cellular systems studied:  cell lines
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MKK7 (human) antisense inhibition of upstream enzyme
KINASE MKK4 (human) antisense inhibition of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
phorbol_ester increase
TNF increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

Y185-p - JNK2 (mouse)
Modsite: TNFMMtPyVVtRYYR SwissProt Entrez-Gene
Orthologous residues
JNK2 (human): Y185‑p, JNK2 iso2 (human): Y185‑p, JNK2 iso3 (human): Y185‑p, JNK2 (mouse): Y185‑p, JNK2 (rat): Y185‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody
Relevant cell lines - cell types - tissues:  U2OS (bone cell) [GR (human)]
Cellular systems studied:  cell lines
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MKK7 (human) antisense inhibition of upstream enzyme
KINASE MKK4 (human) antisense inhibition of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
phorbol_ester increase
TNF increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced