Lee DF, et al. (2008) IKKbeta suppression of TSC1 function links the mTOR pathway with insulin resistance. Int J Mol Med 22, 633-8 18949383

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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S65-p - 4E-BP1 (human) ▲

Modsite: FLMECrNsPVtktPP SwissProt Entrez-Gene Orthologous residues 4E‑BP1 (human): S65‑p, 4E‑BP1 (mouse): S64‑p, 4E‑BP1 (rat): S64‑p, 4E‑BP1 (fruit fly): S65‑p, 4E‑BP1 (cow): S65‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  breast cancer, diabetes mellitus, type 2 diabetes Relevant cell lines - cell types - tissues:  liver, MDA-MB-453 (breast cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes TSC1 (human) decrease no decrease with constitutively active (SSDD) mutant TSC1

S307-p - IRS1 (human) ▲

Modsite: TRRsRtEsItAtsPA SwissProt Entrez-Gene Orthologous residues IRS1 (human): S307‑p, IRS1 (mouse): S302‑p, IRS1 (rat): S302‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  breast cancer, diabetes mellitus, type 2 diabetes Relevant cell lines - cell types - tissues:  liver, MDA-MB-453 (breast cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes TNF increase TSC1 (human) no change compared to control

S636-p - IRS1 (human) ▲

Modsite: sGDyMPMsPKsVSAP SwissProt Entrez-Gene Orthologous residues IRS1 (human): S636‑p, IRS1 (mouse): S632‑p, IRS1 (rat): S632‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  breast cancer, diabetes mellitus, type 2 diabetes Relevant cell lines - cell types - tissues:  liver, MDA-MB-453 (breast cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes TNF increase TSC1 (human) decrease no decrease with const. active (SSDD) TSC1 mutant

S639-p - IRS1 (human) ▲

Modsite: yMPMsPKsVSAPQQI SwissProt Entrez-Gene Orthologous residues IRS1 (human): S639‑p, IRS1 (mouse): S635‑p, IRS1 (rat): S635‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  breast cancer, diabetes mellitus, type 2 diabetes Relevant cell lines - cell types - tissues:  liver, MDA-MB-453 (breast cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes TNF increase TSC1 (human) decrease no decrease with const. active (SSDD) TSC1 mutant

T412-p - p70S6K (human) ▲

Modsite: NQVFLGFtyVAPsVL SwissProt Entrez-Gene Orthologous residues p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  breast cancer, diabetes mellitus, type 2 diabetes Relevant cell lines - cell types - tissues:  liver, MDA-MB-453 (breast cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes TSC1 (human) decrease no decrease with constitutively active (SSDD) mutant TSC1

S473-p - Akt1 (mouse) ▲

Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene Orthologous residues Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  breast cancer, diabetes mellitus, type 2 diabetes Relevant cell lines - cell types - tissues:  liver, MDA-MB-453 (breast cell) Cellular systems studied:  cell lines Species studied:  human

S181-p - IKKB (mouse) ▲

Modsite: DQGsLCTsFVGTLQy SwissProt Entrez-Gene Orthologous residues IKKB (human): S181‑p, IKKB (mouse): S181‑p, IKKB (rat): S181‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  breast cancer, diabetes mellitus, type 2 diabetes Relevant cell lines - cell types - tissues:  liver, MDA-MB-453 (breast cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes high-fat diet increase

S240-p - S6 (mouse) ▲

Modsite: RLssLRAstsKsEss SwissProt Entrez-Gene Orthologous residues S6 (human): S240‑p, S6 (mouse): S240‑p, S6 (rat): S240‑p, S6 (fruit fly): S239‑p, S6 (cow): S240‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  breast cancer, diabetes mellitus, type 2 diabetes Relevant cell lines - cell types - tissues:  liver, MDA-MB-453 (breast cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes high-fat diet increase

S244-p - S6 (mouse) ▲

Modsite: LRAstsKsEssQK__ SwissProt Entrez-Gene Orthologous residues S6 (human): S244‑p, S6 (mouse): S244‑p, S6 (rat): S244‑p, S6 (fruit fly): V243‑p, S6 (cow): S244‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  breast cancer, diabetes mellitus, type 2 diabetes Relevant cell lines - cell types - tissues:  liver, MDA-MB-453 (breast cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes high-fat diet increase

S508-p - TSC1 (mouse) ▲

Modsite: DsPFyRDsLsGsQRK SwissProt Entrez-Gene Orthologous residues TSC1 (human): S511‑p, TSC1 (mouse): S508‑p, TSC1 iso2 (mouse): S507‑p, TSC1 (rat): S511‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  breast cancer, diabetes mellitus, type 2 diabetes Relevant cell lines - cell types - tissues:  liver, MDA-MB-453 (breast cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes high-fat diet increase