Curated Information
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Home > Curated Information Page > PubMed Id: 18757826
Marin TM, et al. (2008) Shp2 negatively regulates growth in cardiomyocytes by controlling focal adhesion kinase/Src and mTOR pathways. Circ Res 103, 813-24 18757826
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S473-p - Akt1 (rat)
Modsite: RPHFPQFsYSASGTA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  myocyte-heart
Cellular systems studied:  primary cultured cells
Species studied:  rat
Comments:  neonatal rat ventricular myocytes
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SHP-2 (rat) decrease
PP2 SHP-2 (rat) inhibit treatment-induced decrease
cyclic_stretch increase
PP2 cyclic_stretch inhibit treatment-induced increase
cyclic_stretch FAK (rat) increase
PP2 no change compared to control
FAK (rat) no change compared to control

T203-p - ERK1 (rat)
Modsite: HDHTGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  myocyte-heart
Cellular systems studied:  primary cultured cells
Species studied:  rat
Comments:  neonatal rat ventricular myocytes
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SHP-2 (rat) increase
PP2 decrease
cyclic_stretch increase
PP2 cyclic_stretch inhibit treatment-induced increase
cyclic_stretch FAK (rat) increase
FAK (rat) no change compared to control

Y205-p - ERK1 (rat)
Modsite: HTGFLtEyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  myocyte-heart
Cellular systems studied:  primary cultured cells
Species studied:  rat
Comments:  neonatal rat ventricular myocytes
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SHP-2 (rat) increase
PP2 decrease
cyclic_stretch increase
PP2 cyclic_stretch inhibit treatment-induced increase
cyclic_stretch FAK (rat) increase
FAK (rat) no change compared to control

T183-p - ERK2 (rat)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  myocyte-heart
Cellular systems studied:  primary cultured cells
Species studied:  rat
Comments:  neonatal rat ventricular myocytes
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SHP-2 (rat) increase
PP2 decrease
cyclic_stretch increase
PP2 cyclic_stretch inhibit treatment-induced increase
cyclic_stretch FAK (rat) increase
FAK (rat) no change compared to control

Y185-p - ERK2 (rat)
Modsite: HtGFLtEyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  myocyte-heart
Cellular systems studied:  primary cultured cells
Species studied:  rat
Comments:  neonatal rat ventricular myocytes
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SHP-2 (rat) increase
PP2 decrease
cyclic_stretch increase
PP2 cyclic_stretch inhibit treatment-induced increase
cyclic_stretch FAK (rat) increase
FAK (rat) no change compared to control

Y397-p - FAK (rat)
Modsite: SVSETDDyAEIIDEE SwissProt Entrez-Gene
Orthologous residues
FAK (human): Y397‑p, FAK iso2 (human): Y216‑p, FAK iso5 (human): Y397‑p, FAK (mouse): Y397‑p, FAK iso2 (mouse): Y428‑p, FAK iso4 (mouse): Y397‑p, FAK iso9 (mouse): , FAK (rat): Y397‑p, FAK (chicken): Y397‑p, FAK iso5 (chicken):
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cyclic_stretch increase
cyclic_stretch increase
cyclic_stretch SHP-2 (rat) no effect upon treatment-induced increase
TFMS cyclic_stretch augment treatment-induced increase
PP2 cyclic_stretch inhibit treatment-induced increase
SHP-2 (rat) decrease
TFMS increase
PP2 no change compared to control

Y861-p - FAK (rat)
Modsite: PTGNQHIyQPVGKPD SwissProt Entrez-Gene
Orthologous residues
FAK (human): Y861‑p, FAK iso2 (human): Y688‑p, FAK iso5 (human): Y861‑p, FAK (mouse): Y861‑p, FAK iso2 (mouse): Y892‑p, FAK iso4 (mouse): Y861‑p, FAK iso9 (mouse): Y169‑p, FAK (rat): Y861‑p, FAK (chicken): Y863‑p, FAK iso5 (chicken): Y169‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  myocyte-heart
Cellular systems studied:  primary cultured cells
Species studied:  rat
Comments:  neonatal rat ventricular myocytes
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SHP-2 (rat) decrease

Y928-p - FAK (rat)
Modsite: DRSNDKVyENVTGLV SwissProt Entrez-Gene
Orthologous residues
FAK (human): Y925‑p, FAK iso2 (human): Y752‑p, FAK iso5 (human): Y938‑p, FAK (mouse): Y925‑p, FAK iso2 (mouse): Y956‑p, FAK iso4 (mouse): Y928‑p, FAK iso9 (mouse): Y233‑p, FAK (rat): Y928‑p, FAK (chicken): Y926‑p, FAK iso5 (chicken): Y232‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  myocyte-heart
Cellular systems studied:  primary cultured cells
Species studied:  rat
Comments:  neonatal rat ventricular myocytes
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SHP-2 (rat) decrease

T412-p - p70S6K (rat)
Modsite: NQVFLGFtYVAPSVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  myocyte-heart
Cellular systems studied:  primary cultured cells
Species studied:  rat
Comments:  neonatal rat ventricular myocytes
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SHP-2 (rat) decrease
SHP-2 (rat) decrease
PP2 SHP-2 (rat) inhibit treatment-induced decrease
cyclic_stretch increase
PP2 cyclic_stretch inhibit treatment-induced increase
cyclic_stretch FAK (rat) increase
PP2 no change compared to control
FAK (rat) no change compared to control

Y546-p - SHP-2 (rat)
Modsite: SKRKGHEyTNIKYSL SwissProt Entrez-Gene
Orthologous residues
SHP‑2 (human): Y542‑p, SHP‑2 iso1 (human): Y546‑p, SHP‑2 (mouse): Y542‑p, SHP‑2 (rat): Y546‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  myocyte-heart
Cellular systems studied:  primary cultured cells
Species studied:  rat
Comments:  neonatal rat ventricular myocytes
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cyclic_stretch increase

Y419-p - Src (rat)
Modsite: RLIEDNEyTARQGAk SwissProt Entrez-Gene
Orthologous residues
Src (human): Y419‑p, Src iso2 (human): Y425‑p, Src (mouse): Y424‑p, Src iso2 (mouse): Y418‑p, Src (rat): Y419‑p, Src (chicken): Y416‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  myocyte-heart
Cellular systems studied:  primary cultured cells
Species studied:  rat
Comments:  neonatal rat ventricular myocytes
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SHP-2 (rat) decrease
cyclic_stretch increase
PP2 cyclic_stretch inhibit treatment-induced increase
cyclic_stretch FAK (rat) increase
PP2 no change compared to control
FAK (rat) no change compared to control

T1466-p - TSC2 (rat)
Modsite: GLRPRGYtISDSAPS SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): T1462‑p, TSC2 iso2 (human): T1419‑p, TSC2 iso3 (human): T1418‑p, TSC2 iso4 (human): T1439‑p, TSC2 (mouse): T1465‑p, TSC2 iso6 (mouse): T1443‑p, TSC2 (rat): T1466‑p, TSC2 iso2 (rat): T1423‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  myocyte-heart
Cellular systems studied:  primary cultured cells
Species studied:  rat
Comments:  neonatal rat ventricular myocytes
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SHP-2 (rat) decrease
SHP-2 (rat) decrease
PP2 SHP-2 (rat) inhibit treatment-induced decrease
cyclic_stretch increase
PP2 cyclic_stretch inhibit treatment-induced increase
cyclic_stretch FAK (rat) increase
PP2 no change compared to control
FAK (rat) no change compared to control