Curated Information
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Home > Curated Information Page > PubMed Id: 19141611
Chen T, et al. (2009) Identification of Zinc-finger BED Domain-containing 3 (Zbed3) as a Novel Axin-interacting Protein That Activates Wnt/{beta}-Catenin Signaling. J Biol Chem 284, 6683-9 19141611
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S33-p - CTNNB1 (mouse)
Modsite: QQQsYLDsGIHsGAT SwissProt Entrez-Gene
Orthologous residues
CTNNB1 (human): S33‑p, CTNNB1 (mouse): S33‑p, CTNNB1 (rat): S33‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) transfection of wild-type enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ZBED3 (mouse) decrease

S37-p - CTNNB1 (mouse)
Modsite: YLDsGIHsGATtTAP SwissProt Entrez-Gene
Orthologous residues
CTNNB1 (human): S37‑p, CTNNB1 (mouse): S37‑p, CTNNB1 (rat): S37‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) transfection of wild-type enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ZBED3 (mouse) decrease

T41-p - CTNNB1 (mouse)
Modsite: GIHsGATtTAPsLSG SwissProt Entrez-Gene
Orthologous residues
CTNNB1 (human): T41‑p, CTNNB1 (mouse): T41‑p, CTNNB1 (rat): T41‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) transfection of wild-type enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ZBED3 (mouse) decrease

S111-p - ZBED3 (mouse)
Modsite: MPCPPPPsPtMAAEG SwissProt Entrez-Gene
Orthologous residues
ZBED3 (human): G120‑p, ZBED3 (mouse): S111‑p, ZBED3 (rat): S110‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK1E (human) transfection of wild-type enzyme
KINASE GSK3B (human) transfection of wild-type enzyme, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
lithium decrease
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
axin 1 (human) Induces co-immunoprecipitation, yeast two-hybrid
Comments:  activates Wnt signaling; interaction beween ZBED3 and axin is important for ZBED3 to inhibit beta catenin (CTNNB1) phosphorylation by GSK3 beta

T113-p - ZBED3 (mouse)
Modsite: CPPPPsPtMAAEGDW SwissProt Entrez-Gene
Orthologous residues
ZBED3 (human): A122‑p, ZBED3 (mouse): T113‑p, ZBED3 (rat): T112‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) transfection of wild-type enzyme, pharmacological inhibitor of upstream enzyme
KINASE CK1E (human) transfection of wild-type enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
lithium decrease
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
axin 1 (human) Induces co-immunoprecipitation, yeast two-hybrid
Comments:  activates Wnt signaling; interaction beween ZBED3 and axin is important for ZBED3 to inhibit beta catenin (CTNNB1) phosphorylation by GSK3 beta