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Home > Curated Information Page > PubMed Id: 16126726
Abbasi S, et al. (2005) The essential role of MEKK3 signaling in angiotensin II-induced calcineurin/nuclear factor of activated T-cells activation. J Biol Chem 280, 36737-46 16126726
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S163-p - RCAN1 (human)
Modsite: PDKQFLIsPPAsPPV SwissProt Entrez-Gene
Orthologous residues
RCAN1 (human): S163‑p, RCAN1 iso2 (human): S108‑p, RCAN1 iso3 (human): S82‑p, RCAN1 (mouse): S161‑p, RCAN1 (rat): S108‑p
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MEKK3 (human) siRNA inhibition of enzyme, genetic knockout/knockin of upstream enzyme, transfection of constitutively active enzyme, mutation in upstream enzyme recognition motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
angiotensin_2 increase
Downstream Regulation
Effect of modification (function):  activity, induced
Effect of modification (process):  transcription, altered
Comments:  Phosphorylation of MCIP at S108/S112 by MEKK3 is required for activation of calcineurin/NFAT signaling.

S167-p - RCAN1 (human)
Modsite: FLIsPPAsPPVGWKQ SwissProt Entrez-Gene
Orthologous residues
RCAN1 (human): S167‑p, RCAN1 iso2 (human): S112‑p, RCAN1 iso3 (human): S86‑p, RCAN1 (mouse): S165‑p, RCAN1 (rat): S112‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  CHO (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MEKK3 (human) siRNA inhibition of enzyme, genetic knockout/knockin of upstream enzyme, transfection of constitutively active enzyme, mutation in upstream enzyme recognition motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
angiotensin_2 increase
Downstream Regulation
Effect of modification (function):  activity, induced
Effect of modification (process):  transcription, altered
Comments:  Phosphorylation of MCIP at S108/S112 by MEKK3 is required for activation of calcineurin/NFAT signaling.

S108-p - RCAN1 (rat)
Modsite: PDKQFLIsPPAsPPV SwissProt Entrez-Gene
Orthologous residues
RCAN1 (human): S163‑p, RCAN1 iso2 (human): S108‑p, RCAN1 iso3 (human): S82‑p, RCAN1 (mouse): S161‑p, RCAN1 (rat): S108‑p

S112-p - RCAN1 (rat)
Modsite: FLIsPPAsPPVGWKQ SwissProt Entrez-Gene
Orthologous residues
RCAN1 (human): S167‑p, RCAN1 iso2 (human): S112‑p, RCAN1 iso3 (human): S86‑p, RCAN1 (mouse): S165‑p, RCAN1 (rat): S112‑p