Curated Information
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Home > Curated Information Page > PubMed Id: 14976552
Lizcano JM, et al. (2004) LKB1 is a master kinase that activates 13 kinases of the AMPK subfamily, including MARK/PAR-1. EMBO J 23, 833-43 14976552
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T205-p - BRSK1 iso2 (human)
Modsite: VGDSLLEtSCGsPHY SwissProt Entrez-Gene
Orthologous residues
BRSK1 (human): T189‑p, BRSK1 iso2 (human): T205‑p, BRSK1 (mouse): T189‑p, BRSK1 (rat): T189‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, peptide sequencing
Relevant cell lines - cell types - tissues:  HeLa (cervical) [LKB1 (human)], MEF (fibroblast) [LKB1 (human)]
Cellular systems studied:  cell lines, primary cells
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE LKB1 (human)

T174-p - BRSK2 (human)
Modsite: VGDSLLEtSCGSPHY SwissProt Entrez-Gene
Orthologous residues
BRSK2 (human): T174‑p, BRSK2 iso2 (human): T174‑p, BRSK2 iso3 (human): T174‑p, BRSK2 iso4 (human): T174‑p, BRSK2 iso5 (human): T220‑p, BRSK2 iso6 (human): , BRSK2 (mouse): T175‑p, BRSK2 (rat): T175‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, peptide sequencing
Relevant cell lines - cell types - tissues:  HeLa (cervical) [LKB1 (human)], MEF (fibroblast) [LKB1 (human)]
Cellular systems studied:  cell lines, primary cells
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE LKB1 (human)

T215-p - MARK1 (human)
Modsite: TVGNKLDtFCGsPPY SwissProt Entrez-Gene
Orthologous residues
MARK1 (human): T215‑p, MARK1 iso3 (human): T193‑p, MARK1 (mouse): T215‑p, MARK1 (rat): T215‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, peptide sequencing
Relevant cell lines - cell types - tissues:  HeLa (cervical) [LKB1 (human)], MEF (fibroblast) [LKB1 (human)]
Cellular systems studied:  cell lines, primary cells
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE LKB1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE LKB1 (human) phospho-antibody, transfection of wild-type enzyme, transfection of inactive enzyme

T208-p - MARK2 (human)
Modsite: tFGNKLDtFCGsPPY SwissProt Entrez-Gene
Orthologous residues
MARK2 (human): T208‑p, MARK2 iso4 (human): T208‑p, MARK2 iso10 (human): T175‑p, MARK2 iso11 (human): T208‑p, MARK2 iso13 (human): T175‑p, MARK2 iso14 (human): T175‑p, MARK2 (mouse): T208‑p, MARK2 iso3 (mouse): T208‑p, MARK2 (rat): T208‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, peptide sequencing
Relevant cell lines - cell types - tissues:  HeLa (cervical) [LKB1 (human)], MEF (fibroblast) [LKB1 (human)]
Cellular systems studied:  cell lines, primary cells
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE LKB1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE LKB1 (human) phospho-antibody, transfection of wild-type enzyme, transfection of inactive enzyme

T211-p - MARK3 (human)
Modsite: TVGGKLDtFCGsPPY SwissProt Entrez-Gene
Orthologous residues
MARK3 (human): T211‑p, MARK3 iso3 (human): T211‑p, MARK3 iso6 (human): T211‑p, MARK3 (mouse): T211‑p, MARK3 (rat): T211‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, peptide sequencing
Relevant cell lines - cell types - tissues:  HeLa (cervical) [LKB1 (human)], MEF (fibroblast) [LKB1 (human)]
Cellular systems studied:  cell lines, primary cells
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE LKB1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE LKB1 (human) phospho-antibody, transfection of wild-type enzyme, transfection of inactive enzyme

S215-p - MARK3 (human)
Modsite: KLDtFCGsPPYAAPE SwissProt Entrez-Gene
Orthologous residues
MARK3 (human): S215‑p, MARK3 iso3 (human): S215‑p, MARK3 iso6 (human): S215‑p, MARK3 (mouse): S215‑p, MARK3 (rat): S215‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, peptide sequencing
Enzymes shown to modify site in vitro
Type Enzyme
KINASE LKB1 (human)

T214-p - MARK4 (human)
Modsite: TLGSKLDtFCGsPPY SwissProt Entrez-Gene
Orthologous residues
MARK4 (human): T214‑p, MARK4 iso2 (human): T214‑p, MARK4 (mouse): T214‑p, MARK4 (rat): T214‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, peptide sequencing
Relevant cell lines - cell types - tissues:  HeLa (cervical) [LKB1 (human)], MEF (fibroblast) [LKB1 (human)]
Cellular systems studied:  cell lines, primary cells
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE LKB1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE LKB1 (human) phospho-antibody, transfection of wild-type enzyme, transfection of inactive enzyme

T167-p - MELK (human)
Modsite: NkDyHLQtCCGsLAY SwissProt Entrez-Gene
Orthologous residues
MELK (human): T167‑p, MELK (mouse): T167‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, peptide sequencing
Relevant cell lines - cell types - tissues:  HeLa (cervical) [LKB1 (human)], MEF (fibroblast) [LKB1 (human)]
Cellular systems studied:  cell lines, primary cells
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE LKB1 (human)

T211-p - NuaK1 (human)
Modsite: QKDKFLQtFCGSPLY SwissProt Entrez-Gene
Orthologous residues
NuaK1 (human): T211‑p, NuaK1 (mouse): T212‑p, NuaK1 (rat): T212‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, peptide sequencing
Relevant cell lines - cell types - tissues:  HeLa (cervical) [LKB1 (human)], MEF (fibroblast) [LKB1 (human)]
Cellular systems studied:  cell lines, primary cells
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE LKB1 (human)

T208-p - NuaK2 (human)
Modsite: HQGKFLQtFCGSPLY SwissProt Entrez-Gene
Orthologous residues
NuaK2 (human): T208‑p, NuaK2 (mouse): T220‑p, NuaK2 (rat): T212‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, peptide sequencing
Relevant cell lines - cell types - tissues:  HeLa (cervical) [LKB1 (human)], MEF (fibroblast) [LKB1 (human)]
Cellular systems studied:  cell lines, primary cells
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE LKB1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE LKB1 (human) phospho-antibody, transfection of wild-type enzyme, transfection of inactive enzyme

T175-p - QIK (human)
Modsite: kSGELLAtWCGSPPY SwissProt Entrez-Gene
Orthologous residues
QIK (human): T175‑p, QIK (mouse): T175‑p, QIK (rat): T175‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, peptide sequencing
Relevant cell lines - cell types - tissues:  HeLa (cervical) [LKB1 (human)], MEF (fibroblast) [LKB1 (human)]
Cellular systems studied:  cell lines, primary cells
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE LKB1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE LKB1 (human) phospho-antibody, transfection of wild-type enzyme, transfection of inactive enzyme

T221-p - QSK (human)
Modsite: TPGQLLKtWCGSPPY SwissProt Entrez-Gene
Orthologous residues
QSK (human): T221‑p, QSK iso6 (human): , QSK iso7 (human): T221‑p, QSK iso8 (human): T221‑p, QSK (mouse): T163‑p, QSK (rat): T221‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, peptide sequencing
Relevant cell lines - cell types - tissues:  HeLa (cervical) [LKB1 (human)], MEF (fibroblast) [LKB1 (human)]
Cellular systems studied:  cell lines, primary cells
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE LKB1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE LKB1 (human) phospho-antibody, transfection of wild-type enzyme, transfection of inactive enzyme

T182-p - SIK (human)
Modsite: kSGEPLStWCGSPPY SwissProt Entrez-Gene
Orthologous residues
SIK (human): T182‑p, SIK (mouse): T182‑p, SIK (rat): T182‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, peptide sequencing
Relevant cell lines - cell types - tissues:  HeLa (cervical) [LKB1 (human)], MEF (fibroblast) [LKB1 (human)]
Cellular systems studied:  cell lines, primary cells
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE LKB1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE LKB1 (human) phospho-antibody, transfection of wild-type enzyme, transfection of inactive enzyme