Curated Information
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Home > Curated Information Page > PubMed Id: 11682625
Frasor J, et al. (2001) PRL-induced ERalpha gene expression is mediated by Janus kinase 2 (Jak2) while signal transducer and activator of transcription 5b (Stat5b) phosphorylation involves Jak2 and a second tyrosine kinase. Mol Endocrinol 15, 1941-52 11682625
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y694-p - STAT5A (rat)
Modsite: LAKAVDGyVKPQIKQ SwissProt Entrez-Gene
Orthologous residues
STAT5A (human): Y694‑p, STAT5A (mouse): Y694‑p, STAT5A iso2 (mouse): , STAT5A (rat): Y694‑p, STAT5A (fruit fly): Y711‑p, STAT5A (sheep): Y694‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast), granulosa
Cellular systems studied:  cell lines
Species studied:  green monkey
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JAK2 (rat) transfection of dominant-negative enzyme, transfection of constitutively active enzyme, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PRL increase
AG490 PRL inhibit treatment-induced increase

Y699-p - STAT5B (rat)
Modsite: TAKAADGyVKPQIKQ SwissProt Entrez-Gene
Orthologous residues
STAT5B (human): Y699‑p, STAT5B (mouse): Y699‑p, STAT5B (rat): Y699‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast), granulosa
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  green monkey, rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JAK2 (rat) transfection of dominant-negative enzyme, transfection of constitutively active enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PRL increase
AG490 PRL no effect upon treatment-induced increase
PP2 PRL no effect upon treatment-induced increase
LY294002 PRL no effect upon treatment-induced increase
LY294002, AG490 PRL inhibit treatment-induced increase