Curated Information
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Home > Curated Information Page > PubMed Id: 11278964
Gatei M, et al. (2001) Ataxia telangiectasia mutated (ATM) kinase and ATM and Rad3 related kinase mediate phosphorylation of Brca1 at distinct and overlapping sites. In vivo assessment using phospho-specific antibodies. J Biol Chem 276, 17276-80 11278964
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S1387-p - BRCA1 (human)
Modsite: EDCSGLSsQSDILTT SwissProt Entrez-Gene
Orthologous residues
BRCA1 (human): S1387‑p, BRCA1 iso6 (human): S284‑p, BRCA1 iso7 (human): S1387‑p, BRCA1 (mouse): S1343‑p, BRCA1 (rat): S1344‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  C3ABR (lymphoblastoid), GM847 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) genetic knockout/knockin of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
UV no change compared to control

S1423-p - BRCA1 (human)
Modsite: AVLEQHGsQPSNSYP SwissProt Entrez-Gene
Orthologous residues
BRCA1 (human): S1423‑p, BRCA1 iso6 (human): S320‑p, BRCA1 iso7 (human): S1423‑p, BRCA1 (mouse): N1379‑p, BRCA1 (rat): S1380‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  C3ABR (lymphoblastoid), GM847 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) genetic knockout/knockin of upstream enzyme
KINASE ATR (human) microscopy-colocalization, transfection of wild-type enzyme, co-immunoprecipitation, transfection of dominant-negative enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
UV increase

S1457-p - BRCA1 (human)
Modsite: SEKAVLTsQKsSEYP SwissProt Entrez-Gene
Orthologous residues
BRCA1 (human): S1457‑p, BRCA1 iso6 (human): S353‑p, BRCA1 iso7 (human): S1478‑p, BRCA1 (mouse): S1413‑p, BRCA1 (rat): S1414‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  C3ABR (lymphoblastoid), GM847 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATR (human) microscopy-colocalization, transfection of wild-type enzyme, co-immunoprecipitation, transfection of dominant-negative enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation no change compared to control
UV increase

S1524-p - BRCA1 (human)
Modsite: LQNRNYPsQEELIKV SwissProt Entrez-Gene
Orthologous residues
BRCA1 (human): S1524‑p, BRCA1 iso6 (human): S420‑p, BRCA1 iso7 (human): S1545‑p, BRCA1 (mouse): S1481‑p, BRCA1 (rat): S1482‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  C3ABR (lymphoblastoid), GM847 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) genetic knockout/knockin of upstream enzyme
KINASE ATR (human) microscopy-colocalization, transfection of wild-type enzyme, co-immunoprecipitation, transfection of dominant-negative enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
UV increase