Curated Information
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Home > Curated Information Page > PubMed Id: 28448822
Ashton NW, et al. (2017) hSSB1 phosphorylation is dynamically regulated by DNA-PK and PPP-family protein phosphatases. DNA Repair (Amst) 54, 30-39 28448822
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T117-p - OBFC2B (human)
Modsite: EPNPEYstQQAPNKA SwissProt Entrez-Gene
Orthologous residues
OBFC2B (human): T117‑p, OBFC2B (mouse): T117‑p, OBFC2B (rat): T117‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human

S134-p - OBFC2B (human)
Modsite: NDSNPSAsQPTTGPS SwissProt Entrez-Gene
Orthologous residues
OBFC2B (human): S134‑p, OBFC2B (mouse): P135‑p, OBFC2B (rat): P134‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE DNAPK (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE DNAPK (human) phospho-antibody, pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
aphidicolin increase
camptothecin increase
ionizing_radiation no change compared to control
hydroxyurea increase
okadaic_acid increase
calyculin_A increase