Curated Information
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Home > Curated Information Page > PubMed Id: 28380355
Calses PC, et al. (2017) DGCR8 Mediates Repair of UV-Induced DNA Damage Independently of RNA Processing. Cell Rep 19, 162-174 28380355
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
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S95-p - DGCR8 (human)
Modsite: DPNCsGHsPRTARHA SwissProt Entrez-Gene
Orthologous residues
DGCR8 (human): S95‑p, DGCR8 (mouse): S95‑p, DGCR8 (rat): S95‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human

S153-p - DGCR8 (human)
Modsite: AECGLLLsPVSGDVH SwissProt Entrez-Gene
Orthologous residues
DGCR8 (human): S153‑p, DGCR8 (mouse): S153‑p, DGCR8 (rat): S153‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE JNK1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JNK1 (human) transfection of wild-type enzyme, pharmacological inhibitor of upstream enzyme, pharmacological activator of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
UVB increase
CDK9 (human) decrease CDK9 siRNA increases
Downstream Regulation
Effect of modification (process):  DNA repair, inhibited

S271-p - DGCR8 (human)
Modsite: EEKyGGDsDHPsDGE SwissProt Entrez-Gene
Orthologous residues
DGCR8 (human): S271‑p, DGCR8 (mouse): S271‑p, DGCR8 (rat): S271‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human

S275-p - DGCR8 (human)
Modsite: GGDsDHPsDGEtsVQ SwissProt Entrez-Gene
Orthologous residues
DGCR8 (human): S275‑p, DGCR8 (mouse): S275‑p, DGCR8 (rat): S275‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human

T371-p - DGCR8 (human)
Modsite: REQssDLtPsGDVsP SwissProt Entrez-Gene
Orthologous residues
DGCR8 (human): T371‑p, DGCR8 (mouse): A371‑p, DGCR8 (rat): A371‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human

S373-p - DGCR8 (human)
Modsite: QssDLtPsGDVsPVk SwissProt Entrez-Gene
Orthologous residues
DGCR8 (human): S373‑p, DGCR8 (mouse): S373‑p, DGCR8 (rat): S373‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human

S377-p - DGCR8 (human)
Modsite: LtPsGDVsPVkPLsR SwissProt Entrez-Gene
Orthologous residues
DGCR8 (human): S377‑p, DGCR8 (mouse): S377‑p, DGCR8 (rat): S377‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human

S434-p - DGCR8 (human)
Modsite: VEVCkDEsVDLEEFR SwissProt Entrez-Gene
Orthologous residues
DGCR8 (human): S434‑p, DGCR8 (mouse): S434‑p, DGCR8 (rat): S434‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human

S619-p - DGCR8 (human)
Modsite: TSKAGLLsPYQILHE SwissProt Entrez-Gene
Orthologous residues
DGCR8 (human): S619‑p, DGCR8 (mouse): S619‑p, DGCR8 (rat): S619‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human