Curated Information
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Home > Curated Information Page > PubMed Id: 15975092
Bianchi M, et al. (2005) Regulation of FAK Ser-722 phosphorylation and kinase activity by GSK3 and PP1 during cell spreading and migration. Biochem J 391, 359-70 15975092
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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S21-p - GSK3A (rat)
Modsite: SGRARtssFAEPGGG SwissProt Entrez-Gene
Orthologous residues
GSK3A (human): S21‑p, GSK3A (mouse): S21‑p, GSK3A (rat): S21‑p, GSK3A (cow): S21‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  fibroblast
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fibronectin no change compared to control

S9-p - GSK3B (rat)
Modsite: SGRPRTTsFAESCKP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  fibroblast
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fibronectin decrease

Y397-p - FAK (chicken)
Modsite: sVsETDDyAEIIDEE SwissProt Entrez-Gene
Orthologous residues
FAK (human): Y397‑p, FAK iso2 (human): Y216‑p, FAK iso5 (human): Y397‑p, FAK (mouse): Y397‑p, FAK iso2 (mouse): Y428‑p, FAK iso4 (mouse): Y397‑p, FAK iso9 (mouse): , FAK (rat): Y397‑p, FAK (chicken): Y397‑p, FAK iso5 (chicken):
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), fibroblast, MEF (fibroblast)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cell_adhesion increase
fibronectin increase
lithium fibronectin no effect upon treatment-induced increase
U0126 fibronectin no effect upon treatment-induced increase
seliciclib fibronectin no effect upon treatment-induced increase

Y576-p - FAK (chicken)
Modsite: RyMEDSTyyKASKGK SwissProt Entrez-Gene
Orthologous residues
FAK (human): Y576‑p, FAK iso2 (human): Y424‑p, FAK iso5 (human): Y576‑p, FAK (mouse): Y576‑p, FAK iso2 (mouse): Y607‑p, FAK iso4 (mouse): Y576‑p, FAK iso9 (mouse): , FAK (rat): Y576‑p, FAK (chicken): Y576‑p, FAK iso5 (chicken):
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), fibroblast, MEF (fibroblast)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fibronectin increase

Y577-p - FAK (chicken)
Modsite: yMEDSTyyKASKGKL SwissProt Entrez-Gene
Orthologous residues
FAK (human): Y577‑p, FAK iso2 (human): Y425‑p, FAK iso5 (human): Y577‑p, FAK (mouse): Y577‑p, FAK iso2 (mouse): Y608‑p, FAK iso4 (mouse): Y577‑p, FAK iso9 (mouse): , FAK (rat): Y577‑p, FAK (chicken): Y577‑p, FAK iso5 (chicken):
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), fibroblast, MEF (fibroblast)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fibronectin increase

S722-p - FAK (chicken)
Modsite: PSRPGYPsPRSsEGF SwissProt Entrez-Gene
Orthologous residues
FAK (human): S722‑p, FAK iso2 (human): S570‑p, FAK iso5 (human): S722‑p, FAK (mouse): S722‑p, FAK iso2 (mouse): S753‑p, FAK iso4 (mouse): S722‑p, FAK iso9 (mouse): S30‑p, FAK (rat): S722‑p, FAK (chicken): S722‑p, FAK iso5 (chicken): S30‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), fibroblast, MEF (fibroblast)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse, rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE GSK3B (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) mutation in upstream enzyme recognition motif, siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme, pharmacological activator of upstream enzyme, co-immunoprecipitation, phospho-antibody, modification site within consensus motif
PHOSPHATASE PPP1CA (human) pharmacological inhibitor of upstream enzyme, co-immunoprecipitation, phospho-antibody, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cell_adhesion increase
fibronectin increase
lithium fibronectin inhibit treatment-induced increase
U0126 fibronectin no effect upon treatment-induced increase
seliciclib fibronectin no effect upon treatment-induced increase
SB216763 fibronectin inhibit treatment-induced increase
kenpaullone fibronectin inhibit treatment-induced increase
okadaic_acid increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, inhibited, molecular association, regulation
Effect of modification (process):  cell motility, altered
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PPP1CA (human) Induces pull-down assay

S726-p - FAK (chicken)
Modsite: GYPsPRSsEGFYPsP SwissProt Entrez-Gene
Orthologous residues
FAK (human): S726‑p, FAK iso2 (human): S574‑p, FAK iso5 (human): S726‑p, FAK (mouse): S726‑p, FAK iso2 (mouse): S757‑p, FAK iso4 (mouse): S726‑p, FAK iso9 (mouse): S34‑p, FAK (rat): S726‑p, FAK (chicken): S726‑p, FAK iso5 (chicken): S34‑p
Downstream Regulation
Effect of modification (function):  phosphorylation
Comments:  primes for the GSK3-beta phosphorylation of S722

S911-p - FAK (chicken)
Modsite: KIKPQEIsPPPTANL SwissProt Entrez-Gene
Orthologous residues
FAK (human): S910‑p, FAK iso2 (human): S737‑p, FAK iso5 (human): S923‑p, FAK (mouse): S910‑p, FAK iso2 (mouse): S941‑p, FAK iso4 (mouse): S913‑p, FAK iso9 (mouse): S218‑p, FAK (rat): S913‑p, FAK (chicken): S911‑p, FAK iso5 (chicken): S217‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), fibroblast, MEF (fibroblast)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cell_adhesion no change compared to control
fibronectin no change compared to control
okadaic_acid no change compared to control