Curated Information
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Home > Curated Information Page > PubMed Id: 19153595
Zhang B, et al. (2009) The TAK1-JNK cascade is required for IRF3 function in the innate immune response. Cell Res 19, 412-28 19153595
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S173-p - IRF3 (human)
Modsite: PCPQPLRsPsLDNPt SwissProt Entrez-Gene
Orthologous residues
IRF3 (human): S173‑p, IRF3 (mouse): S171‑p, IRF3 (rat): S172‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE JNK1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JNK1 (human) activation of upstream enzyme, transfection of wild-type enzyme, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SP600125 decrease
TAK1 (human) increase
Downstream Regulation
Effect of modification (function):  phosphorylation
Effect of modification (process):  transcription, altered

S396-p - IRF3 (human)
Modsite: NtVDLHIsNsHPLsL SwissProt Entrez-Gene
Orthologous residues
IRF3 (human): S396‑p, IRF3 (mouse): S388‑p, IRF3 (rat): S390‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE IKKB (human) transfection of wild-type enzyme
KINASE TBK1 (human) transfection of wild-type enzyme
Downstream Regulation
Effect of modification (process):  transcription, altered

S398-p - IRF3 (human)
Modsite: VDLHIsNsHPLsLts SwissProt Entrez-Gene
Orthologous residues
IRF3 (human): S398‑p, IRF3 (mouse): S390‑p, IRF3 (rat): S392‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TBK1 (human) transfection of wild-type enzyme
KINASE IKKB (human) transfection of wild-type enzyme
Downstream Regulation
Effect of modification (process):  transcription, altered

S402-p - IRF3 (human)
Modsite: IsNsHPLsLtsDQYK SwissProt Entrez-Gene
Orthologous residues
IRF3 (human): S402‑p, IRF3 (mouse): S394‑p, IRF3 (rat): S396‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TBK1 (human) transfection of wild-type enzyme
KINASE IKKB (human) transfection of wild-type enzyme
Downstream Regulation
Effect of modification (process):  transcription, altered

T404-p - IRF3 (human)
Modsite: NsHPLsLtsDQYKAY SwissProt Entrez-Gene
Orthologous residues
IRF3 (human): T404‑p, IRF3 (mouse): T396‑p, IRF3 (rat): T398‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TBK1 (human) transfection of wild-type enzyme
KINASE IKKB (human) transfection of wild-type enzyme
Downstream Regulation
Effect of modification (process):  transcription, altered

S405-p - IRF3 (human)
Modsite: sHPLsLtsDQYKAYL SwissProt Entrez-Gene
Orthologous residues
IRF3 (human): S405‑p, IRF3 (mouse): S397‑p, IRF3 (rat): S399‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE IKKB (human) transfection of wild-type enzyme
KINASE TBK1 (human) transfection of wild-type enzyme
Downstream Regulation
Effect of modification (process):  transcription, altered