Zhang B, et al. (2009) The TAK1-JNK cascade is required for IRF3 function in the innate immune response. Cell Res 19, 412-28 19153595

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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S173-p - IRF3 (human) ▲

Modsite: PCPQPLRsPsLDNPt SwissProt Entrez-Gene Orthologous residues IRF3 (human): S173‑p, IRF3 (mouse): S171‑p, IRF3 (rat): S172‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE JNK1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE JNK1 (human) activation of upstream enzyme, transfection of wild-type enzyme, pharmacological inhibitor of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes SP600125 decrease TAK1 (human) increase Downstream Regulation Effect of modification (function):  phosphorylation Effect of modification (process):  transcription, altered

S396-p - IRF3 (human) ▲

Modsite: NtVDLHIsNsHPLsL SwissProt Entrez-Gene Orthologous residues IRF3 (human): S396‑p, IRF3 (mouse): S388‑p, IRF3 (rat): S390‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE IKKB (human) transfection of wild-type enzyme KINASE TBK1 (human) transfection of wild-type enzyme Downstream Regulation Effect of modification (process):  transcription, altered

S398-p - IRF3 (human) ▲

Modsite: VDLHIsNsHPLsLts SwissProt Entrez-Gene Orthologous residues IRF3 (human): S398‑p, IRF3 (mouse): S390‑p, IRF3 (rat): S392‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE TBK1 (human) transfection of wild-type enzyme KINASE IKKB (human) transfection of wild-type enzyme Downstream Regulation Effect of modification (process):  transcription, altered

S402-p - IRF3 (human) ▲

Modsite: IsNsHPLsLtsDQYK SwissProt Entrez-Gene Orthologous residues IRF3 (human): S402‑p, IRF3 (mouse): S394‑p, IRF3 (rat): S396‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE TBK1 (human) transfection of wild-type enzyme KINASE IKKB (human) transfection of wild-type enzyme Downstream Regulation Effect of modification (process):  transcription, altered

T404-p - IRF3 (human) ▲

Modsite: NsHPLsLtsDQYKAY SwissProt Entrez-Gene Orthologous residues IRF3 (human): T404‑p, IRF3 (mouse): T396‑p, IRF3 (rat): T398‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE TBK1 (human) transfection of wild-type enzyme KINASE IKKB (human) transfection of wild-type enzyme Downstream Regulation Effect of modification (process):  transcription, altered

S405-p - IRF3 (human) ▲

Modsite: sHPLsLtsDQYKAYL SwissProt Entrez-Gene Orthologous residues IRF3 (human): S405‑p, IRF3 (mouse): S397‑p, IRF3 (rat): S399‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE IKKB (human) transfection of wild-type enzyme KINASE TBK1 (human) transfection of wild-type enzyme Downstream Regulation Effect of modification (process):  transcription, altered