Curated Information
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Home > Curated Information Page > PubMed Id: 28319066
Zhang Y, et al. (2017) Nucleus accumbens-associated protein-1 promotes glycolysis and survival of hypoxic tumor cells via the HDAC4-HIF-1α axis. Oncogene 36, 4171-4181 28319066
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S246-p - HDAC4 (human)
Modsite: FPLRkTAsEPNLKLR SwissProt Entrez-Gene
Orthologous residues
HDAC4 (human): S246‑p, HDAC4 (mouse): S245‑p, HDAC4 (rat): S245‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  ovarian cancer
Relevant cell lines - cell types - tissues:  A2780 (ovarian), ES2 (ovarian), HEK293T (epithelial), SKOV-3 (ovarian)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypoxia NAC1 (human) decrease NAC1 siRNA increase
Downstream Regulation
Effect of modification (function):  intracellular localization, protein degradation

K10-ac - HIF1A (human)
Modsite: GAGGANDkkkISSER SwissProt Entrez-Gene
Orthologous residues
HIF1A (human): K10‑ac, HIF1A (mouse): K10‑ac, HIF1A (rat): K10‑ac
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  ovarian cancer
Relevant cell lines - cell types - tissues:  A2780 (ovarian), ES2 (ovarian), HEK293T (epithelial), SKOV-3 (ovarian)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  protein degradation
Effect of modification (process):  transcription, inhibited

K11-ac - HIF1A (human)
Modsite: AGGANDkkkISSERR SwissProt Entrez-Gene
Orthologous residues
HIF1A (human): K11‑ac, HIF1A (mouse): K11‑ac, HIF1A (rat): K11‑ac
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  ovarian cancer
Relevant cell lines - cell types - tissues:  A2780 (ovarian), ES2 (ovarian), HEK293T (epithelial), SKOV-3 (ovarian)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  protein degradation
Effect of modification (process):  transcription, inhibited

K12-ac - HIF1A (human)
Modsite: GGANDkkkISSERRk SwissProt Entrez-Gene
Orthologous residues
HIF1A (human): K12‑ac, HIF1A (mouse): K12‑ac, HIF1A (rat): K12‑ac
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  ovarian cancer
Relevant cell lines - cell types - tissues:  A2780 (ovarian), ES2 (ovarian), HEK293T (epithelial), SKOV-3 (ovarian)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  protein degradation
Effect of modification (process):  transcription, inhibited

K19-ac - HIF1A (human)
Modsite: kISSERRkEkSRDAA SwissProt Entrez-Gene
Orthologous residues
HIF1A (human): K19‑ac, HIF1A (mouse): K19‑ac, HIF1A (rat): K19‑ac
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  ovarian cancer
Relevant cell lines - cell types - tissues:  A2780 (ovarian), ES2 (ovarian), HEK293T (epithelial), SKOV-3 (ovarian)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  protein degradation
Effect of modification (process):  transcription, inhibited

K21-ac - HIF1A (human)
Modsite: SSERRkEkSRDAARS SwissProt Entrez-Gene
Orthologous residues
HIF1A (human): K21‑ac, HIF1A (mouse): K21‑ac, HIF1A (rat): K21‑ac
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  ovarian cancer
Relevant cell lines - cell types - tissues:  A2780 (ovarian), ES2 (ovarian), HEK293T (epithelial), SKOV-3 (ovarian)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  protein degradation
Effect of modification (process):  transcription, inhibited