Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus®
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 18838385
Gao MH, et al. (2008) Adenylyl cyclase type VI increases Akt activity and phospholamban phosphorylation in cardiac myocytes. J Biol Chem 283, 33527-35 18838385
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

T308-p - Akt1 (rat)
Modsite: KDGATMKtFCGTPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ADCY6 (mouse) increase

S473-p - Akt1 (rat)
Modsite: RPHFPQFsYSASGTA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  myocyte-heart
Cellular systems studied:  primary cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ADCY6 (mouse) increase
ADCY6 (mouse) ADCY6 (mouse) no effect upon treatment-induced increase foscarnet
CPA ADCY6 (mouse) decrease
ADCY6 (mouse) augment treatment-induced decrease SQ22536
PKI ADCY6 (mouse) ADCY6 (mouse) no effect upon treatment-induced increase
8-Rp-cAMP ADCY6 (mouse) ADCY6 (mouse) no effect upon treatment-induced increase
H-89 ADCY6 (mouse) ADCY6 (mouse) no effect upon treatment-induced increase
ADCY6 (mouse) increase
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  nuclear localization of Akt upon ADCY6 expression.

S21-p - GSK3A (rat)
Modsite: SGRARtssFAEPGGG SwissProt Entrez-Gene
Orthologous residues
GSK3A (human): S21‑p, GSK3A (mouse): S21‑p, GSK3A (rat): S21‑p, GSK3A (cow): S21‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ADCY6 (mouse) increase
isoproterenol ADCY6 (mouse) no effect upon treatment-induced increase

S9-p - GSK3B (rat)
Modsite: SGRPRTTsFAESCKP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ADCY6 (mouse) increase
isoproterenol ADCY6 (mouse) no effect upon treatment-induced increase

T198-p - PKACA (rat)
Modsite: RVKGRtWtLCGTPEY SwissProt Entrez-Gene
Orthologous residues
PKACA (human): T198‑p, PKACA iso2 (human): T191‑p, PKACA (mouse): T198‑p, PKACA (rat): T198‑p, PKACA (pig): T198‑p, PKACA (dog): T197‑p, PKACA (cow): T198‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  myocyte-heart
Cellular systems studied:  primary cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoproterenol no change compared to control
NKH_477 decrease
isoproterenol ADCY6 (mouse) decrease
NKH_477 ADCY6 (mouse) decrease

S16-p - PLB (rat)
Modsite: RSAIRRAstIEMPQQ SwissProt Entrez-Gene
Orthologous residues
PLB (human): S16‑p, PLB (mouse): S16‑p, PLB (rat): S16‑p, PLB (rabbit): S16‑p, PLB (pig): S16‑p, PLB (dog): S16‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  myocyte-heart
Cellular systems studied:  primary cells
Species studied:  rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (rat) pharmacological inhibitor of upstream enzyme, phospho-antibody
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoproterenol inhibit treatment-induced decrease
colforsin increase
isoproterenol ADCY6 (mouse) increase
colforsin ADCY6 (mouse) increase
ADCY6 (mouse) increase
serum increase slight increase
serum ADCY6 (mouse) ADCY6 (mouse) augment treatment-induced increase
ADCY6 (mouse) augment treatment-induced increase CGP2072A
ICI-118,551 ADCY6 (mouse) augment treatment-induced increase
ADCY6 (mouse) increase
ADCY6 (mouse) no effect upon treatment-induced increase foscarnet
CPA ADCY6 (mouse) augment treatment-induced increase
ADCY6 (mouse) no change compared to control SQ22563
Akt1 (human) increase
Akt-I-1 ADCY6 (mouse) ADCY6 (mouse) inhibit treatment-induced increase
Akt-I-1,2 ADCY6 (mouse) ADCY6 (mouse) inhibit treatment-induced increase
PKI ADCY6 (mouse) ADCY6 (mouse) no effect upon treatment-induced increase
8-Rp-cAMP ADCY6 (mouse) no change compared to control
H-89 ADCY6 (mouse) no change compared to control

T17-p - PLB (rat)
Modsite: SAIRRAstIEMPQQA SwissProt Entrez-Gene
Orthologous residues
PLB (human): T17‑p, PLB (mouse): T17‑p, PLB (rat): T17‑p, PLB (rabbit): T17‑p, PLB (pig): T17‑p, PLB (dog): T17‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  myocyte-heart
Cellular systems studied:  primary cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoproterenol increase
colforsin no change compared to control
isoproterenol ADCY6 (mouse) increase
colforsin ADCY6 (human) no change compared to control