Curated Information
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Home > Curated Information Page > PubMed Id: 14657031
Kraft C, et al. (2003) Mitotic regulation of the human anaphase-promoting complex by phosphorylation. EMBO J 22, 6598-609 14657031
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S202-p - APC1 (human)
Modsite: SHEVPPGsPREPLPT SwissProt Entrez-Gene
Orthologous residues
APC1 (human): S202‑p, APC1 (mouse): L202‑p, APC1 (rat): L202‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S286-p - APC1 (human)
Modsite: ENVVLkFsEQGGtPQ SwissProt Entrez-Gene
Orthologous residues
APC1 (human): S286‑p, APC1 (mouse): P286‑p, APC1 (rat): P286‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T291-p - APC1 (human)
Modsite: kFsEQGGtPQNVATS SwissProt Entrez-Gene
Orthologous residues
APC1 (human): T291‑p, APC1 (mouse): T291‑p, APC1 (rat): T291‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
KINASE PLK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S355-p - APC1 (human)
Modsite: AALsrAHsPALGVHs SwissProt Entrez-Gene
Orthologous residues
APC1 (human): S355‑p, APC1 (mouse): S355‑p, APC1 (rat): S355‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
KINASE PLK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase
Downstream Regulation
Effect of modification (function):  activity, induced

S373-p - APC1 (human)
Modsite: VQRFNIssHNQsPkR SwissProt Entrez-Gene
Orthologous residues
APC1 (human): S373‑p, APC1 (mouse): S373‑p, APC1 (rat): S373‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
KINASE PLK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S377-p - APC1 (human)
Modsite: NIssHNQsPkRHSIS SwissProt Entrez-Gene
Orthologous residues
APC1 (human): S377‑p, APC1 (mouse): S377‑p, APC1 (rat): S377‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
KINASE PLK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T520-p - APC1 (human)
Modsite: GLPAPsLtMsNtMPR SwissProt Entrez-Gene
Orthologous residues
APC1 (human): T520‑p, APC1 (mouse): T520‑p, APC1 (rat): T520‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hydroxyurea increase

T530-p - APC1 (human)
Modsite: NtMPRPstPLDGVst SwissProt Entrez-Gene
Orthologous residues
APC1 (human): T530‑p, APC1 (mouse): T530‑p, APC1 (rat): T530‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
KINASE PLK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hydroxyurea increase

T537-p - APC1 (human)
Modsite: tPLDGVstPkPLsKL SwissProt Entrez-Gene
Orthologous residues
APC1 (human): T537‑p, APC1 (mouse): T537‑p, APC1 (rat): T537‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S547-p - APC1 (human)
Modsite: PLsKLLGsLDEVVLL SwissProt Entrez-Gene
Orthologous residues
APC1 (human): S547‑p, APC1 (mouse): S547‑p, APC1 (rat): S547‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
KINASE PLK1 (human)

S555-p - APC1 (human)
Modsite: LDEVVLLsPVPELRD SwissProt Entrez-Gene
Orthologous residues
APC1 (human): S555‑p, APC1 (mouse): S555‑p, APC1 (rat): S555‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)

S569-p - APC1 (human)
Modsite: DsskLHDsLyNEDCT SwissProt Entrez-Gene
Orthologous residues
APC1 (human): S569‑p, APC1 (mouse): S569‑p, APC1 (rat): S569‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hydroxyurea increase

Y571-p - APC1 (human)
Modsite: skLHDsLyNEDCTFQ SwissProt Entrez-Gene
Orthologous residues
APC1 (human): Y571‑p, APC1 (mouse): Y571‑p, APC1 (rat): Y571‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S688-p - APC1 (human)
Modsite: FDFEGsLsPVIAPkk SwissProt Entrez-Gene
Orthologous residues
APC1 (human): S688‑p, APC1 (mouse): S688‑p, APC1 (rat): S688‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
KINASE PLK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T701-p - APC1 (human)
Modsite: kkARPsEtGsDDDWE SwissProt Entrez-Gene
Orthologous residues
APC1 (human): T701‑p, APC1 (mouse): T701‑p, APC1 (rat): T701‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)
KINASE CDK1 (human)

S731-p - APC1 (human)
Modsite: LNRsLCLsPSEASQM SwissProt Entrez-Gene
Orthologous residues
APC1 (human): S731‑p, APC1 (mouse): T731‑p, APC1 (rat): N731‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hydroxyurea increase

S314-p - APC2 (human)
Modsite: DGPARPAsPEAGNtL SwissProt Entrez-Gene
Orthologous residues
APC2 (human): S314‑p, APC2 iso2 (human): S314‑p, APC2 (mouse): S329‑p, APC2 (rat): S31‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S534-p - APC2 (human)
Modsite: LLHQFsFsPEREIRN SwissProt Entrez-Gene
Orthologous residues
APC2 (human): S534‑p, APC2 iso2 (human): S531‑p, APC2 (mouse): S549‑p, APC2 (rat): S251‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

Y469-p - APC4 (human)
Modsite: LYNRKGkyFNVERVG SwissProt Entrez-Gene
Orthologous residues
APC4 (human): Y469‑p, APC4 iso3 (human): Y470‑p, APC4 (mouse): Y469‑p, APC4 (rat): Y469‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S779-p - APC4 (human)
Modsite: kEEVLsEsEAENQQA SwissProt Entrez-Gene
Orthologous residues
APC4 (human): S779‑p, APC4 iso3 (human): S780‑p, APC4 (mouse): S779‑p, APC4 (rat): S779‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
KINASE PLK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S195-p - APC5 (human)
Modsite: EKEELDVsVREEEVS SwissProt Entrez-Gene
Orthologous residues
APC5 (human): S195‑p, APC5 iso2 (human): S74‑p, APC5 (mouse): S180‑p, APC5 (rat): S180‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase
hydroxyurea increase

S51-p - APC7 (human)
Modsite: MAAAGLHsNVRLLss SwissProt Entrez-Gene
Orthologous residues
APC7 (human): S51‑p, APC7 (mouse): S17‑p, APC7 (rat): S17‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)

S57-p - APC7 (human)
Modsite: HsNVRLLssLLLtMs SwissProt Entrez-Gene
Orthologous residues
APC7 (human): S57‑p, APC7 (mouse): S23‑p, APC7 (rat): S23‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)

S64-p - APC7 (human)
Modsite: ssLLLtMsNNNPELF SwissProt Entrez-Gene
Orthologous residues
APC7 (human): S64‑p, APC7 (mouse): S30‑p, APC7 (rat): S30‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)

S112-p - CDC16 (human)
Modsite: EkYLkDEsGFkDPSS SwissProt Entrez-Gene
Orthologous residues
CDC16 (human): S112‑p, CDC16 (mouse): N112‑p, CDC16 (rat): S112‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
KINASE PLK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S490-p - CDC16 (human)
Modsite: SAIGYIHsLMGNFEN SwissProt Entrez-Gene
Orthologous residues
CDC16 (human): S490‑p, CDC16 (mouse): S490‑p, CDC16 (rat): S490‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S560-p - CDC16 (human)
Modsite: kTLkNIIsPPWDFRE SwissProt Entrez-Gene
Orthologous residues
CDC16 (human): S560‑p, CDC16 (mouse): S560‑p, CDC16 (rat): S560‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T581-p - CDC16 (human)
Modsite: TAEETGLtPLETsRK SwissProt Entrez-Gene
Orthologous residues
CDC16 (human): T581‑p, CDC16 (mouse): A581‑p, CDC16 (rat): A581‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S595-p - CDC16 (human)
Modsite: KtPDSRPsLEEtFEI SwissProt Entrez-Gene
Orthologous residues
CDC16 (human): S595‑p, CDC16 (mouse): N595‑p, CDC16 (rat): S595‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T599-p - CDC16 (human)
Modsite: SRPsLEEtFEIEMNE SwissProt Entrez-Gene
Orthologous residues
CDC16 (human): T599‑p, CDC16 (mouse): T599‑p, CDC16 (rat): T599‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T70-p - CDC20 (human)
Modsite: SSSkVQttPskPGGD SwissProt Entrez-Gene
Orthologous residues
CDC20 (human): T70‑p, CDC20 (mouse): T70‑p, CDC20 (rat): T70‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T106-p - CDC20 (human)
Modsite: NQPENSQtPTkKEHQ SwissProt Entrez-Gene
Orthologous residues
CDC20 (human): T106‑p, CDC20 (mouse): T106‑p, CDC20 (rat): T106‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

Y273-p - CDC23 (human)
Modsite: VSQIAVAyHNIRDID SwissProt Entrez-Gene
Orthologous residues
CDC23 (human): Y273‑p, CDC23 (mouse): Y273‑p, CDC23 (rat): Y273‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T562-p - CDC23 (human)
Modsite: QLRNQGEtPttEVPA SwissProt Entrez-Gene
Orthologous residues
CDC23 (human): T562‑p, CDC23 (mouse): T562‑p, CDC23 (rat): T562‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
KINASE PLK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T565-p - CDC23 (human)
Modsite: NQGEtPttEVPAPFF SwissProt Entrez-Gene
Orthologous residues
CDC23 (human): T565‑p, CDC23 (mouse): S565‑p, CDC23 (rat): S565‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
KINASE PLK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T205-p - CDC27 (human)
Modsite: PEtVLtEtPQDtIEL SwissProt Entrez-Gene
Orthologous residues
CDC27 (human): T205‑p, CDC27 iso2 (human): T205‑p, CDC27 (mouse): T205‑p, CDC27 iso2 (mouse): T186‑p, CDC27 (rat): T205‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)
KINASE CDK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T209-p - CDC27 (human)
Modsite: LtEtPQDtIELNRLN SwissProt Entrez-Gene
Orthologous residues
CDC27 (human): T209‑p, CDC27 iso2 (human): T209‑p, CDC27 (mouse): T209‑p, CDC27 iso2 (mouse): T190‑p, CDC27 (rat): T209‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)
KINASE CDK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S222-p - CDC27 (human)
Modsite: LNLEssNsKYSLNTD SwissProt Entrez-Gene
Orthologous residues
CDC27 (human): S222‑p, CDC27 iso2 (human): S222‑p, CDC27 (mouse): S222‑p, CDC27 iso2 (mouse): S203‑p, CDC27 (rat): S222‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)

T244-p - CDC27 (human)
Modsite: SAVIsPDtVPLGTGT SwissProt Entrez-Gene
Orthologous residues
CDC27 (human): T244‑p, CDC27 iso2 (human): T244‑p, CDC27 (mouse): N244‑p, CDC27 iso2 (mouse): N225‑p, CDC27 (rat): N244‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T289-p - CDC27 (human)
Modsite: FGILPLEtPsPGDGs SwissProt Entrez-Gene
Orthologous residues
CDC27 (human): T289‑p, CDC27 iso2 (human): T289‑p, CDC27 (mouse): T289‑p, CDC27 iso2 (mouse): T270‑p, CDC27 (rat): T289‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)

S291-p - CDC27 (human)
Modsite: ILPLEtPsPGDGsYL SwissProt Entrez-Gene
Orthologous residues
CDC27 (human): S291‑p, CDC27 iso2 (human): S291‑p, CDC27 (mouse): S291‑p, CDC27 iso2 (mouse): S272‑p, CDC27 (rat): S291‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S296-p - CDC27 (human)
Modsite: tPsPGDGsYLQNYTN SwissProt Entrez-Gene
Orthologous residues
CDC27 (human): S296‑p, CDC27 iso2 (human): S296‑p, CDC27 (mouse): S296‑p, CDC27 iso2 (mouse): S277‑p, CDC27 (rat): S296‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human

S312-p - CDC27 (human)
Modsite: PPVIDVPstGAPSKk SwissProt Entrez-Gene
Orthologous residues
CDC27 (human): S312‑p, CDC27 iso2 (human): S312‑p, CDC27 (mouse): P312‑p, CDC27 iso2 (mouse): P293‑p, CDC27 (rat): S312‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)

T313-p - CDC27 (human)
Modsite: PVIDVPstGAPSKkS SwissProt Entrez-Gene
Orthologous residues
CDC27 (human): T313‑p, CDC27 iso2 (human): T313‑p, CDC27 (mouse): T313‑p, CDC27 iso2 (mouse): N294‑p, CDC27 (rat): T313‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T329-p - CDC27 (human)
Modsite: ARIGQTGtkSVFsQs SwissProt Entrez-Gene
Orthologous residues
CDC27 (human): T329‑p, CDC27 iso2 (human): T335‑p, CDC27 (mouse): T329‑p, CDC27 iso2 (mouse): T310‑p, CDC27 (rat): A329‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)

T343-p - CDC27 (human)
Modsite: sGNsREVtPILAQTQ SwissProt Entrez-Gene
Orthologous residues
CDC27 (human): T343‑p, CDC27 iso2 (human): T349‑p, CDC27 (mouse): T343‑p, CDC27 iso2 (mouse): T324‑p, CDC27 (rat): T343‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)

S426-p - CDC27 (human)
Modsite: TQPNINDsLEItkLD SwissProt Entrez-Gene
Orthologous residues
CDC27 (human): S426‑p, CDC27 iso2 (human): S432‑p, CDC27 (mouse): S427‑p, CDC27 iso2 (mouse): , CDC27 (rat): S427‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)
KINASE CDK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T430-p - CDC27 (human)
Modsite: INDsLEItkLDssII SwissProt Entrez-Gene
Orthologous residues
CDC27 (human): T430‑p, CDC27 iso2 (human): T436‑p, CDC27 (mouse): T431‑p, CDC27 iso2 (mouse): , CDC27 (rat): T431‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)
KINASE CDK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

S434-p - CDC27 (human)
Modsite: LEItkLDssIIsEGk SwissProt Entrez-Gene
Orthologous residues
CDC27 (human): S434‑p, CDC27 iso2 (human): S440‑p, CDC27 (mouse): S435‑p, CDC27 iso2 (mouse): , CDC27 (rat): S435‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
KINASE PLK1 (human)

S435-p - CDC27 (human)
Modsite: EItkLDssIIsEGkI SwissProt Entrez-Gene
Orthologous residues
CDC27 (human): S435‑p, CDC27 iso2 (human): S441‑p, CDC27 (mouse): S436‑p, CDC27 iso2 (mouse): , CDC27 (rat): S436‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
KINASE PLK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase

T446-p - CDC27 (human)
Modsite: EGkIStItPQIQAFN SwissProt Entrez-Gene
Orthologous residues
CDC27 (human): T446‑p, CDC27 iso2 (human): T452‑p, CDC27 (mouse): T447‑p, CDC27 iso2 (mouse): , CDC27 (rat): T447‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (human)
KINASE CDK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase