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Home > Curated Information Page > PubMed Id: 27738173
Mylona A, et al. (2016) Opposing effects of Elk-1 multisite phosphorylation shape its response to ERK activation. Science 354, 233-237 27738173
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T337-p - ELK1 (mouse)
Modsite: GGQGPERtPGSGTSS SwissProt Entrez-Gene
Orthologous residues
ELK1 (human): T336‑p, ELK1 (mouse): T337‑p, ELK1 (rat): T335‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [ELK1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  triple knockout of Elk1, Elk3, Elk4 in MEFs
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK2 (human)
Comments:  fast phosphorylation of T369, S384; intermediate T354, T364, S390; slow T337, T418, S423
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  transcription, inhibited
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
MED16 (mouse) Disrupts co-immunoprecipitation
MED23 (mouse) Disrupts co-immunoprecipitation
Trap100 (mouse) Disrupts co-immunoprecipitation

T354-p - ELK1 (mouse)
Modsite: QAPGPALtPSLLPTH SwissProt Entrez-Gene
Orthologous residues
ELK1 (human): T353‑p, ELK1 (mouse): T354‑p, ELK1 (rat): T352‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [ELK1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  triple knockout of Elk1, Elk3, Elk4 in MEFs
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK2 (human)
Comments:  fast phosphorylation of T369, S384; intermediate T354, T364, S390; slow T337, T418, S423
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
MED16 (mouse) Induces co-immunoprecipitation
Trap100 (mouse) Induces co-immunoprecipitation
MED23 (mouse) Induces co-immunoprecipitation

T364-p - ELK1 (mouse)
Modsite: LLPTHTLtPVLLtPS SwissProt Entrez-Gene
Orthologous residues
ELK1 (human): T363‑p, ELK1 (mouse): T364‑p, ELK1 (rat): T362‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [ELK1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  triple knockout of Elk1, Elk3, Elk4 in MEFs
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK2 (human)
Comments:  fast phosphorylation of T369, S384; intermediate T354, T364, S390; slow T337, T418, S423
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
MED16 (mouse) Induces co-immunoprecipitation
Trap100 (mouse) Induces co-immunoprecipitation
MED23 (mouse) Induces co-immunoprecipitation

T369-p - ELK1 (mouse)
Modsite: TLtPVLLtPSSLPPS SwissProt Entrez-Gene
Orthologous residues
ELK1 (human): T368‑p, ELK1 (mouse): T369‑p, ELK1 (rat): T367‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [ELK1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  triple knockout of Elk1, Elk3, Elk4 in MEFs
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK2 (human)
Comments:  fast phosphorylation of T369, S384; intermediate T354, T364, S390; slow T337, T418, S423
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
MED23 (mouse) Induces co-immunoprecipitation
Trap100 (mouse) Induces co-immunoprecipitation
MED16 (mouse) Induces co-immunoprecipitation

S384-p - ELK1 (mouse)
Modsite: IHFWSTLsPIAPRsP SwissProt Entrez-Gene
Orthologous residues
ELK1 (human): S383‑p, ELK1 (mouse): S384‑p, ELK1 (rat): S382‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [ELK1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  triple knockout of Elk1, Elk3, Elk4 in MEFs
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK2 (human)
Comments:  fast phosphorylation of T369, S384; intermediate T354, T364, S390; slow T337, T418, S423
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Trap100 (mouse) Induces co-immunoprecipitation
MED23 (mouse) Induces co-immunoprecipitation
MED16 (mouse) Induces co-immunoprecipitation

S390-p - ELK1 (mouse)
Modsite: LsPIAPRsPAKLSFQ SwissProt Entrez-Gene
Orthologous residues
ELK1 (human): S389‑p, ELK1 (mouse): S390‑p, ELK1 (rat): S388‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [ELK1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  triple knockout of Elk1, Elk3, Elk4 in MEFs
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK2 (human)
Comments:  fast phosphorylation of T369, S384; intermediate T354, T364, S390; slow T337, T418, S423
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
MED23 (mouse) Induces co-immunoprecipitation
Trap100 (mouse) Induces co-immunoprecipitation
MED16 (mouse) Induces co-immunoprecipitation

T418-p - ELK1 (mouse)
Modsite: ISVDGLStPVVLsPG SwissProt Entrez-Gene
Orthologous residues
ELK1 (human): T417‑p, ELK1 (mouse): T418‑p, ELK1 (rat): T416‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [ELK1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  triple knockout of Elk1, Elk3, Elk4 in MEFs
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK2 (human)
Comments:  fast phosphorylation of T369, S384; intermediate T354, T364, S390; slow T337, T418, S423
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  transcription, inhibited
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Trap100 (mouse) Disrupts co-immunoprecipitation
MED16 (mouse) Disrupts co-immunoprecipitation
MED23 (mouse) Disrupts co-immunoprecipitation

S423-p - ELK1 (mouse)
Modsite: LStPVVLsPGPQKP_ SwissProt Entrez-Gene
Orthologous residues
ELK1 (human): S422‑p, ELK1 (mouse): S423‑p, ELK1 (rat): S421‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [ELK1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  triple knockout of Elk1, Elk3, Elk4 in MEFs
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK2 (human)
Comments:  fast phosphorylation of T369, S384; intermediate T354, T364, S390; slow T337, T418, S423
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  transcription, inhibited
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
MED23 (mouse) Disrupts co-immunoprecipitation
Trap100 (mouse) Disrupts co-immunoprecipitation
MED16 (mouse) Disrupts co-immunoprecipitation