Curated Information
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Home > Curated Information Page > PubMed Id: 17050683
Solinas G, et al. (2006) Saturated fatty acids inhibit induction of insulin gene transcription by JNK-mediated phosphorylation of insulin-receptor substrates. Proc Natl Acad Sci U S A 103, 16454-9 17050683
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S473-p - Akt1 (mouse)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  beta-pancreas, hepatocyte-liver
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  mouse islet cells
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
palmitate insulin inhibit treatment-induced increase
JNK_inhibitor_I insulin no effect upon treatment-induced increase D-JNKi
palmitate no change compared to control
insulin increase
siRNA insulin IRS1 (human) no effect upon treatment-induced increase
siRNA IRS1 (mouse) no change compared to control
siRNA insulin IRS2 (mouse) no effect upon treatment-induced increase
siRNA IRS2 (mouse) no change compared to control
siRNA insulin IRS1 (mouse), IRS2 (mouse) inhibit treatment-induced increase
siRNA IRS1 (mouse), IRS2 (mouse) no change compared to control

S302-p - IRS1 (mouse)
Modsite: TRRSRtEsITAtsPA SwissProt Entrez-Gene
Orthologous residues
IRS1 (human): S307‑p, IRS1 (mouse): S302‑p, IRS1 (rat): S302‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  beta-pancreas, hepatocyte-liver
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  mouse islet cells
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
palmitate increase
JNK_inhibitor_I palmitate inhibit treatment-induced increase

T348-p - IRS2 (rat)
Modsite: RTDsLAAtPPAAKCT SwissProt Entrez-Gene
Orthologous residues
IRS2 (human): T350‑p, IRS2 (mouse): T347‑p, IRS2 (rat): T348‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  beta-pancreas, hepatocyte-liver
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  mouse islet cells
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JNK1 (rat) co-immunoprecipitation