Curated Information
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Home > Curated Information Page > PubMed Id: 19172738
Brummer T, et al. (2008) Phosphorylation-dependent binding of 14-3-3 terminates signalling by the Gab2 docking protein. EMBO J 27, 2305-16 19172738
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T308-p - Akt1 (human)
Modsite: kDGAtMKtFCGtPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  COS (fibroblast), MCF-10A (breast cell) [GAB2 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase
EGF GAB2 (human) augment treatment-induced increase Gab2 S210A/T391A single or double mutations

S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  COS (fibroblast), MCF-10A (breast cell) [GAB2 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase
EGF GAB2 (human) augment treatment-induced increase Gab2 S210A/T391A single or double mutations

T202-p - ERK1 (human)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  COS (fibroblast), MCF-10A (breast cell) [GAB2 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  green monkey, human

Y204-p - ERK1 (human)
Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  COS (fibroblast), MCF-10A (breast cell) [GAB2 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  green monkey, human

T185-p - ERK2 (human)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  COS (fibroblast), MCF-10A (breast cell) [GAB2 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  green monkey, human

Y187-p - ERK2 (human)
Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  COS (fibroblast), MCF-10A (breast cell) [GAB2 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  green monkey, human

S133-p - GAB2 (human)
Modsite: DSLRNVSsAGHGPRs SwissProt Entrez-Gene
Orthologous residues
GAB2 (human): S133‑p, GAB2 iso2 (human): S95‑p, GAB2 (mouse): S135‑p, GAB2 (rat): S135‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-10A (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

S140-p - GAB2 (human)
Modsite: sAGHGPRssPAELSS SwissProt Entrez-Gene
Orthologous residues
GAB2 (human): S140‑p, GAB2 iso2 (human): S102‑p, GAB2 (mouse): S142‑p, GAB2 (rat): S142‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-10A (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

S141-p - GAB2 (human)
Modsite: AGHGPRssPAELSSs SwissProt Entrez-Gene
Orthologous residues
GAB2 (human): S141‑p, GAB2 iso2 (human): S103‑p, GAB2 (mouse): S143‑p, GAB2 (rat): S143‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-10A (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

S148-p - GAB2 (human)
Modsite: sPAELSSssQHLLRE SwissProt Entrez-Gene
Orthologous residues
GAB2 (human): S148‑p, GAB2 iso2 (human): S110‑p, GAB2 (mouse): S150‑p, GAB2 (rat): S150‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-10A (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

S149-p - GAB2 (human)
Modsite: PAELSSssQHLLRER SwissProt Entrez-Gene
Orthologous residues
GAB2 (human): S149‑p, GAB2 iso2 (human): S111‑p, GAB2 (mouse): , GAB2 (rat):
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-10A (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

S159-p - GAB2 (human)
Modsite: LLRERKSsAPSHsSQ SwissProt Entrez-Gene
Orthologous residues
GAB2 (human): S159‑p, GAB2 iso2 (human): S121‑p, GAB2 (mouse): S160‑p, GAB2 (rat): S160‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  COS (fibroblast), MCF-10A (breast cell), MCF-10A (breast cell) [GAB2 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

S164-p - GAB2 (human)
Modsite: KSsAPSHsSQPTLFt SwissProt Entrez-Gene
Orthologous residues
GAB2 (human): S164‑p, GAB2 iso2 (human): S126‑p, GAB2 (mouse): S165‑p, GAB2 (rat): S165‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-10A (breast cell)
Cellular systems studied:  cell lines
Species studied:  human

S210-p - GAB2 (human)
Modsite: AENARsAsFsQGTRA SwissProt Entrez-Gene
Orthologous residues
GAB2 (human): S210‑p, GAB2 iso2 (human): S172‑p, GAB2 (mouse): S211‑p, GAB2 (rat): S211‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  COS (fibroblast), MCF-10A (breast cell), MCF-10A (breast cell) [GAB2 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase
wortmannin EGF inhibit treatment-induced increase
LY294002 EGF inhibit treatment-induced increase
Akt-I-1,2 EGF inhibit treatment-induced increase
H-89 EGF inhibit treatment-induced increase
rapamycin EGF no effect upon treatment-induced increase
U0126 EGF no effect upon treatment-induced increase
Go_6976 EGF no effect upon treatment-induced increase
Go_6850 EGF no effect upon treatment-induced increase
KN-62 EGF no effect upon treatment-induced increase
Y27632 EGF no effect upon treatment-induced increase
mPKI EGF no effect upon treatment-induced increase
PIK3CA (human) increase activated
insulin increase
Downstream Regulation
Effect of modification (function):  activity, inhibited, intracellular localization, molecular association, regulation, phosphorylation
Effect of modification (process):  cell growth, altered
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (human) Induces molecular association, regulation co-immunoprecipitation
SHC1 (human) Disrupts molecular association, regulation co-immunoprecipitation
GRB2 (human) Disrupts molecular association, regulation co-immunoprecipitation
EGFR (human) Disrupts molecular association, regulation co-immunoprecipitation

S218-p - GAB2 (human)
Modsite: FsQGTRAsFLMRsDT SwissProt Entrez-Gene
Orthologous residues
GAB2 (human): S218‑p, GAB2 iso2 (human): S180‑p, GAB2 (mouse): , GAB2 (rat):
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-10A (breast cell)
Cellular systems studied:  cell lines
Species studied:  human

S223-p - GAB2 (human)
Modsite: RAsFLMRsDTAVQKL SwissProt Entrez-Gene
Orthologous residues
GAB2 (human): S223‑p, GAB2 iso2 (human): S185‑p, GAB2 (mouse): S220‑p, GAB2 (rat): S220‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-10A (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

S264-p - GAB2 (human)
Modsite: HNTEFRDstyDLPRS SwissProt Entrez-Gene
Orthologous residues
GAB2 (human): S264‑p, GAB2 iso2 (human): S226‑p, GAB2 (mouse): S261‑p, GAB2 (rat): S261‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-10A (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

T278-p - GAB2 (human)
Modsite: SLASHGHtKGsLTGs SwissProt Entrez-Gene
Orthologous residues
GAB2 (human): T278‑p, GAB2 iso2 (human): T240‑p, GAB2 (mouse): T275‑p, GAB2 (rat): T275‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-10A (breast cell)
Cellular systems studied:  cell lines
Species studied:  human

S281-p - GAB2 (human)
Modsite: SHGHtKGsLTGsEtD SwissProt Entrez-Gene
Orthologous residues
GAB2 (human): S281‑p, GAB2 iso2 (human): S243‑p, GAB2 (mouse): S278‑p, GAB2 (rat): S278‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-10A (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

T287-p - GAB2 (human)
Modsite: GsLTGsEtDNEDVyt SwissProt Entrez-Gene
Orthologous residues
GAB2 (human): T287‑p, GAB2 iso2 (human): T249‑p, GAB2 (mouse): T284‑p, GAB2 (rat): T284‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-10A (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

Y293-p - GAB2 (human)
Modsite: EtDNEDVytFKTPSN SwissProt Entrez-Gene
Orthologous residues
GAB2 (human): Y293‑p, GAB2 iso2 (human): Y255‑p, GAB2 (mouse): Y290‑p, GAB2 (rat): Y290‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-10A (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

T331-p - GAB2 (human)
Modsite: yQIPRTFtLDKNHNA SwissProt Entrez-Gene
Orthologous residues
GAB2 (human): T331‑p, GAB2 iso2 (human): T293‑p, GAB2 (mouse): T328‑p, GAB2 (rat): T328‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-10A (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

T385-p - GAB2 (human)
Modsite: NSRSVAAtIPRRNtL SwissProt Entrez-Gene
Orthologous residues
GAB2 (human): T385‑p, GAB2 iso2 (human): T347‑p, GAB2 (mouse): T382‑p, GAB2 (rat): T382‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-10A (breast cell)
Cellular systems studied:  cell lines
Species studied:  human

T391-p - GAB2 (human)
Modsite: AtIPRRNtLPAMDNS SwissProt Entrez-Gene
Orthologous residues
GAB2 (human): T391‑p, GAB2 iso2 (human): T353‑p, GAB2 (mouse): T388‑p, GAB2 (rat): T388‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  COS (fibroblast), MCF-10A (breast cell), MCF-10A (breast cell) [GAB2 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase
wortmannin EGF inhibit treatment-induced increase
LY294002 EGF inhibit treatment-induced increase
Akt-I-1,2 EGF inhibit treatment-induced increase
H-89 EGF inhibit treatment-induced increase
rapamycin EGF no effect upon treatment-induced increase
U0126 EGF no effect upon treatment-induced increase
Go_6976 EGF no effect upon treatment-induced increase
Go_6850 EGF no effect upon treatment-induced increase
KN-62 EGF no effect upon treatment-induced increase
Y27632 EGF no effect upon treatment-induced increase
mPKI EGF no effect upon treatment-induced increase
PIK3CA (human) increase activated
insulin increase
Downstream Regulation
Effect of modification (function):  activity, inhibited, intracellular localization, molecular association, regulation, phosphorylation
Effect of modification (process):  cell growth, altered
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SHC1 (human) Disrupts molecular association, regulation co-immunoprecipitation
EGFR (human) Disrupts molecular association, regulation co-immunoprecipitation
GRB2 (human) Disrupts molecular association, regulation co-immunoprecipitation
14-3-3 beta (human) Induces molecular association, regulation co-immunoprecipitation

S405-p - GAB2 (human)
Modsite: SRLHRAssCEtyEyP SwissProt Entrez-Gene
Orthologous residues
GAB2 (human): S405‑p, GAB2 iso2 (human): S367‑p, GAB2 (mouse): S402‑p, GAB2 (rat): S402‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-10A (breast cell)
Cellular systems studied:  cell lines
Species studied:  human

S480-p - GAB2 (human)
Modsite: QsVyIPMsPGAHHFD SwissProt Entrez-Gene
Orthologous residues
GAB2 (human): S480‑p, GAB2 iso2 (human): S442‑p, GAB2 (mouse): S469‑p, GAB2 (rat): G469‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-10A (breast cell)
Cellular systems studied:  cell lines
Species studied:  human

S543-p - GAB2 (human)
Modsite: IDELPFksPItKSWs SwissProt Entrez-Gene
Orthologous residues
GAB2 (human): S543‑p, GAB2 iso2 (human): S505‑p, GAB2 (mouse): S532‑p, GAB2 (rat): S532‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-10A (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

S622-p - GAB2 (human)
Modsite: LALDFQPssPsPHRK SwissProt Entrez-Gene
Orthologous residues
GAB2 (human): S622‑p, GAB2 iso2 (human): S584‑p, GAB2 (mouse): G611‑p, GAB2 (rat): G611‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-10A (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

S623-p - GAB2 (human)
Modsite: ALDFQPssPsPHRKP SwissProt Entrez-Gene
Orthologous residues
GAB2 (human): S623‑p, GAB2 iso2 (human): S585‑p, GAB2 (mouse): S612‑p, GAB2 (rat): S612‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-10A (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase

T412-p - p70S6K (human)
Modsite: NQVFLGFtyVAPsVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  COS (fibroblast), MCF-10A (breast cell) [GAB2 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase
wortmannin EGF inhibit treatment-induced increase
rapamycin EGF inhibit treatment-induced increase