Curated Information
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Home > Curated Information Page > PubMed Id: 28008135
Zibrova D, et al. (2017) GFAT1 phosphorylation by AMPK promotes VEGF-induced angiogenesis. Biochem J 474, 983-1001 28008135
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S243-p - GFAT iso2 (human)
Modsite: CNLsRVDsttCLFPV SwissProt Entrez-Gene
Orthologous residues
GFAT (human): S261‑p, GFAT iso2 (human): S243‑p, GFAT (mouse): S259‑p, GFAT iso2 (mouse): S243‑p, GFAT (rat): S243‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), HUVEC (endothelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phenformin increase
A-769662 increase
acadesine increase
VEGF increase
Downstream Regulation
Effect of modification (function):  O-GlcNAc glycosylation
Effect of modification (process):  cell differentiation, induced
Comments:  promotes angiogenesis

S43-p - ARMC10 (mouse)
Modsite: RRLRPsRsAEDLtDG SwissProt Entrez-Gene
Orthologous residues
ARMC10 (human): S45‑p, ARMC10 iso2 (human): S45‑p, ARMC10 (mouse): S43‑p, ARMC10 (rat): S43‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S695-p - ATP6V0A2 (mouse)
Modsite: YTLVRKDsEEEVsLL SwissProt Entrez-Gene
Orthologous residues
ATP6V0A2 (human): S695‑p, ATP6V0A2 iso2 (human): , ATP6V0A2 (mouse): S695‑p, ATP6V0A2 (rat): S695‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S529-p - BCLAF1 (mouse)
Modsite: KsIFREEsPLRIKMI SwissProt Entrez-Gene
Orthologous residues
BCLAF1 (human): S531‑p, BCLAF1 iso2 (human): S529‑p, BCLAF1 iso3 (human): S529‑p, BCLAF1 iso4 (human): S358‑p, BCLAF1 (mouse): S529‑p, BCLAF1 (rat): S531‑p, BCLAF1 iso3 (rat): S672‑p, BCLAF1 (cow): S531‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

T620-p - CDK12 (mouse)
Modsite: AAIPHLKtstLPPLP SwissProt Entrez-Gene
Orthologous residues
CDK12 (human): T624‑p, CDK12 iso2 (human): T624‑p, CDK12 iso3 (human): T623‑p, CDK12 (mouse): T620‑p, CDK12 iso3 (mouse): T620‑p, CDK12 (rat): T620‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S621-p - CDK12 (mouse)
Modsite: AIPHLKtstLPPLPL SwissProt Entrez-Gene
Orthologous residues
CDK12 (human): S625‑p, CDK12 iso2 (human): S625‑p, CDK12 iso3 (human): S624‑p, CDK12 (mouse): S621‑p, CDK12 iso3 (mouse): S621‑p, CDK12 (rat): S621‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

T622-p - CDK12 (mouse)
Modsite: IPHLKtstLPPLPLP SwissProt Entrez-Gene
Orthologous residues
CDK12 (human): T626‑p, CDK12 iso2 (human): T626‑p, CDK12 iso3 (human): T625‑p, CDK12 (mouse): T622‑p, CDK12 iso3 (mouse): T622‑p, CDK12 (rat): T622‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S640-p - CDK12 (mouse)
Modsite: PGDDDMDsPKEtLPs SwissProt Entrez-Gene
Orthologous residues
CDK12 (human): S644‑p, CDK12 iso2 (human): S644‑p, CDK12 iso3 (human): S643‑p, CDK12 (mouse): S640‑p, CDK12 iso3 (mouse): S640‑p, CDK12 (rat): S640‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S352-p - DCAMKL1 iso2 (mouse)
Modsite: RDLyRPLssDDLDsV SwissProt Entrez-Gene
Orthologous residues
DCAMKL1 (human): , DCAMKL1 iso2 (human): , DCAMKL1 iso5 (human): S352‑p, DCAMKL1 (mouse): , DCAMKL1 iso2 (mouse): S352‑p, DCAMKL1 iso3 (mouse): , DCAMKL1 (rat):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S353-p - DCAMKL1 iso2 (mouse)
Modsite: DLyRPLssDDLDsVG SwissProt Entrez-Gene
Orthologous residues
DCAMKL1 (human): , DCAMKL1 iso2 (human): , DCAMKL1 iso5 (human): S353‑p, DCAMKL1 (mouse): , DCAMKL1 iso2 (mouse): S353‑p, DCAMKL1 iso3 (mouse): , DCAMKL1 (rat):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S508-p - DDEF1 (mouse)
Modsite: VHISRIQsLELDKLG SwissProt Entrez-Gene
Orthologous residues
DDEF1 (human): S493‑p, DDEF1 iso2 (human): S496‑p, DDEF1 (mouse): S508‑p, DDEF1 (rat): S505‑p, DDEF1 (cow): S496‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

T516-p - DDEF1 (mouse)
Modsite: LELDKLGtsELLLAK SwissProt Entrez-Gene
Orthologous residues
DDEF1 (human): T501‑p, DDEF1 iso2 (human): T504‑p, DDEF1 (mouse): T516‑p, DDEF1 (rat): T513‑p, DDEF1 (cow): T504‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S517-p - DDEF1 (mouse)
Modsite: ELDKLGtsELLLAKN SwissProt Entrez-Gene
Orthologous residues
DDEF1 (human): S502‑p, DDEF1 iso2 (human): S505‑p, DDEF1 (mouse): S517‑p, DDEF1 (rat): S514‑p, DDEF1 (cow): S505‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S634-p - FXR2 (mouse)
Modsite: PLGRTEPsEDSLSGQ SwissProt Entrez-Gene
Orthologous residues
FXR2 (human): S634‑p, FXR2 (mouse): S634‑p, FXR2 iso5 (mouse): S635‑p, FXR2 (rat): S636‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S259-p - GFAT (mouse)
Modsite: CGLsRVDsttCLFPV SwissProt Entrez-Gene
Orthologous residues
GFAT (human): S261‑p, GFAT iso2 (human): S243‑p, GFAT (mouse): S259‑p, GFAT iso2 (mouse): S243‑p, GFAT (rat): S243‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), HUVEC (endothelial), MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  human, mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AMPKA1 (mouse)
KINASE AMPKA2 (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE AMPKA1 (mouse) genetic knockout/knockin of upstream enzyme, pharmacological activator of upstream enzyme
KINASE AMPKA2 (mouse) genetic knockout/knockin of upstream enzyme, pharmacological activator of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phenformin increase
A-769662 increase
acadesine increase
VEGF increase

S312-p - GRP58 (mouse)
Modsite: ktFSHELsDFGLEsT SwissProt Entrez-Gene
Orthologous residues
GRP58 (human): S312‑p, GRP58 (mouse): S312‑p, GRP58 (rat): S312‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S318-p - GRP58 (mouse)
Modsite: LsDFGLEsTTGEVPV SwissProt Entrez-Gene
Orthologous residues
GRP58 (human): S318‑p, GRP58 (mouse): S318‑p, GRP58 (rat): S318‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S15-p - HSP27 (mouse)
Modsite: FsLLrsPsWEPFRDW SwissProt Entrez-Gene
Orthologous residues
HSP27 (human): S15‑p, HSP27 (mouse): S15‑p, HSP27 (rat): S15‑p, HSP27 (pig): S15‑p, HSP27 (hamster): S15‑p, HSP27 (chicken): S15‑p, HSP27 (dog): S15‑p, HSP27 (cow): S15‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S648-p - HUWE1 (mouse)
Modsite: PAMRRRRssDPLGDT SwissProt Entrez-Gene
Orthologous residues
HUWE1 (human): S648‑p, HUWE1 iso2 (human): S648‑p, HUWE1 (mouse): S648‑p, HUWE1 (rat):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S2471-p - IGF2R (mouse)
Modsite: TAPAKLVsFHDDsDE SwissProt Entrez-Gene
Orthologous residues
IGF2R (human): S2479‑p, IGF2R (mouse): S2471‑p, IGF2R (rat): S2467‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S2476-p - IGF2R (mouse)
Modsite: LVsFHDDsDEDLLHI SwissProt Entrez-Gene
Orthologous residues
IGF2R (human): S2484‑p, IGF2R (mouse): S2476‑p, IGF2R (rat): S2472‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S210-p - MCT1 (mouse)
Modsite: VkLEKLKskEsLQEA SwissProt Entrez-Gene
Orthologous residues
MCT1 (human): S210‑p, MCT1 iso4 (human): S190‑p, MCT1 (mouse): S210‑p, MCT1 (rat): S210‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S213-p - MCT1 (mouse)
Modsite: EKLKskEsLQEAGks SwissProt Entrez-Gene
Orthologous residues
MCT1 (human): S213‑p, MCT1 iso4 (human): S193‑p, MCT1 (mouse): S213‑p, MCT1 (rat): S213‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S2032-p - MYO18A (mouse)
Modsite: sIsRPRFsHsyLsDs SwissProt Entrez-Gene
Orthologous residues
MYO18A (human): S2036‑p, MYO18A iso2 (human): S1705‑p, MYO18A iso3 (human): S1984‑p, MYO18A iso4 (human): S2021‑p, MYO18A (mouse): S2032‑p, MYO18A iso4 (mouse): S1980‑p, MYO18A iso6 (mouse): S2044‑p, MYO18A iso8 (mouse): , MYO18A (rat): S2036‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S2037-p - MYO18A (mouse)
Modsite: RFsHsyLsDsDtEAK SwissProt Entrez-Gene
Orthologous residues
MYO18A (human): S2041‑p, MYO18A iso2 (human): S1710‑p, MYO18A iso3 (human): S1989‑p, MYO18A iso4 (human): S2026‑p, MYO18A (mouse): S2037‑p, MYO18A iso4 (mouse): S1985‑p, MYO18A iso6 (mouse): S2049‑p, MYO18A iso8 (mouse): , MYO18A (rat): S2041‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S14-p - PGAM1 (mouse)
Modsite: VLIRHGEsAWNLENR SwissProt Entrez-Gene
Orthologous residues
PGAM1 (human): S14‑p, PGAM1 (mouse): S14‑p, PGAM1 (rat): S14‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S428-p - PIK4CB (mouse)
Modsite: NRIRsTRsVENLPEC SwissProt Entrez-Gene
Orthologous residues
PIK4CB (human): S428‑p, PIK4CB iso2 (human): S413‑p, PIK4CB (mouse): S428‑p, PIK4CB iso2 (mouse): S413‑p, PIK4CB (rat): S428‑p, PIK4CB iso5 (rat): S413‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S1155-p - PMCA1 (mouse)
Modsite: PEFRIEDsEPHIPLI SwissProt Entrez-Gene
Orthologous residues
PMCA1 (human): S1155‑p, PMCA1 iso1 (human): S1193‑p, PMCA1 (mouse): S1155‑p, PMCA1 (rat): S1193‑p, PMCA1 iso3 (rat): S1155‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

T1165-p - PMCA1 (mouse)
Modsite: HIPLIDDtDAEDDAP SwissProt Entrez-Gene
Orthologous residues
PMCA1 (human): T1165‑p, PMCA1 iso1 (human): T1203‑p, PMCA1 (mouse): T1165‑p, PMCA1 (rat): T1203‑p, PMCA1 iso3 (rat): T1165‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

T1173-p - PMCA1 (mouse)
Modsite: DAEDDAPtKRNssPP SwissProt Entrez-Gene
Orthologous residues
PMCA1 (human): T1173‑p, PMCA1 iso1 (human): T1211‑p, PMCA1 (mouse): T1173‑p, PMCA1 (rat): T1211‑p, PMCA1 iso3 (rat): T1173‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S13-p - RABGAP1 (mouse)
Modsite: sVGKISVsSDSVSTL SwissProt Entrez-Gene
Orthologous residues
RABGAP1 (human): S13‑p, RABGAP1 (mouse): S13‑p, RABGAP1 (rat): S13‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S524-p - SAPLa iso3 (mouse)
Modsite: tTCHIHsssDDEIDF SwissProt Entrez-Gene
Orthologous residues
SAPLa (human): S524‑p, SAPLa iso2 (human): S524‑p, SAPLa iso5 (human): S524‑p, SAPLa (mouse): , SAPLa iso3 (mouse): S524‑p, SAPLa (rat): S524‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S525-p - SAPLa iso3 (mouse)
Modsite: TCHIHsssDDEIDFK SwissProt Entrez-Gene
Orthologous residues
SAPLa (human): S525‑p, SAPLa iso2 (human): S525‑p, SAPLa iso5 (human): S525‑p, SAPLa (mouse): , SAPLa iso3 (mouse): S525‑p, SAPLa (rat): S525‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S253-p - SKI2W (mouse)
Modsite: TPLVRAssLEDLVLK SwissProt Entrez-Gene
Orthologous residues
SKI2W (human): S256‑p, SKI2W (mouse): S253‑p, SKI2W (rat): S250‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S2070-p - SRm300 (mouse)
Modsite: SSRMsCFsRPsMsPt SwissProt Entrez-Gene
Orthologous residues
SRm300 (human): S2118‑p, SRm300 iso2 (human): , SRm300 (mouse): S2070‑p, SRm300 iso2 (mouse): S1974‑p, SRm300 iso3 (mouse): S2070‑p, SRm300 (rat): S2074‑p, SRm300 (cow): S2083‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S2075-p - SRm300 (mouse)
Modsite: CFsRPsMsPtPLDRC SwissProt Entrez-Gene
Orthologous residues
SRm300 (human): S2123‑p, SRm300 iso2 (human): , SRm300 (mouse): S2075‑p, SRm300 iso2 (mouse): S1979‑p, SRm300 iso3 (mouse): S2075‑p, SRm300 (rat): S2079‑p, SRm300 (cow): S2088‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S16-p - STMN1 (mouse)
Modsite: KELEKRAsGQAFELI SwissProt Entrez-Gene
Orthologous residues
STMN1 (human): S16‑p, STMN1 iso2 (human): S16‑p, STMN1 (mouse): S16‑p, STMN1 (rat): S16‑p, STMN1 (chicken): S16‑p, STMN1 (cow): S16‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S25-p - STMN1 (mouse)
Modsite: QAFELILsPRskEsV SwissProt Entrez-Gene
Orthologous residues
STMN1 (human): S25‑p, STMN1 iso2 (human): S25‑p, STMN1 (mouse): S25‑p, STMN1 (rat): S25‑p, STMN1 (chicken): G25‑p, STMN1 (cow): S25‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

T177-p - TPD52L2 (mouse)
Modsite: PVMRNsAtFKsFEDR SwissProt Entrez-Gene
Orthologous residues
TPD52L2 (human): T163‑p, TPD52L2 iso2 (human): T143‑p, TPD52L2 iso3 (human): T166‑p, TPD52L2 iso4 (human): T157‑p, TPD52L2 iso5 (human): T177‑p, TPD52L2 iso7 (human): T186‑p, TPD52L2 (mouse): T177‑p, TPD52L2 iso2 (mouse): T143‑p, TPD52L2 iso3 (mouse): T186‑p, TPD52L2 iso4 (mouse): T177‑p, TPD52L2 (rat): T177‑p, TPD52L2 (cow): T186‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S1388-p - TSC2 (mouse)
Modsite: QPLSKssssPELQTL SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S1387‑p, TSC2 iso2 (human): S1344‑p, TSC2 iso3 (human): S1343‑p, TSC2 iso4 (human): S1364‑p, TSC2 (mouse): S1388‑p, TSC2 iso6 (mouse): S1366‑p, TSC2 (rat): S1389‑p, TSC2 iso2 (rat): S1346‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S225-p - U5-200kD (mouse)
Modsite: GEVREEAsDDDMEGD SwissProt Entrez-Gene
Orthologous residues
U5‑200kD (human): S225‑p, U5‑200kD (mouse): S225‑p, U5‑200kD (rat): S225‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type

S46-p - UBE1 (mouse)
Modsite: NGMAKNGsEADIDES SwissProt Entrez-Gene
Orthologous residues
UBE1 (human): S46‑p, UBE1 (mouse): S46‑p, UBE1 (rat): S46‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  MEF (fibroblast), MEF (fibroblast) [AMPKA1 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  AMPKA1(-/-)/AMPKA2(-/-), AMPKA1/AMPKA2 wild type