Curated Information
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Home > Curated Information Page > PubMed Id: 28196893
Kuipers HF, et al. (2017) Phosphorylation of αB-crystallin supports reactive astrogliosis in demyelination. Proc Natl Acad Sci U S A 114, E1745-E1754 28196893
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S45-p - CRYAB (human)
Modsite: FPTSTsLsPFYLrPP SwissProt Entrez-Gene
Orthologous residues
CRYAB (human): S45‑p, CRYAB (mouse): S45‑p, CRYAB (rat): S45‑p, CRYAB (rabbit): S45‑p, CRYAB (pig): S45‑p, CRYAB (cow): S45‑p

S59-p - CRYAB (human)
Modsite: PsFLRAPsWFDTGLS SwissProt Entrez-Gene
Orthologous residues
CRYAB (human): S59‑p, CRYAB (mouse): S59‑p, CRYAB (rat): S59‑p, CRYAB (rabbit): S59‑p, CRYAB (pig): S59‑p, CRYAB (cow): S59‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  brain
Cellular systems studied:  tissue
Species studied:  human
Associated Diseases
Diseases Alterations Comments
multiple sclerosis increased

S45-p - CRYAB (mouse)
Modsite: FSTATSLsPFYLRPP SwissProt Entrez-Gene
Orthologous residues
CRYAB (human): S45‑p, CRYAB (mouse): S45‑p, CRYAB (rat): S45‑p, CRYAB (rabbit): S45‑p, CRYAB (pig): S45‑p, CRYAB (cow): S45‑p
Characterization
Methods used to characterize site in vivo immunoassay, phospho-antibody
Relevant cell lines - cell types - tissues:  astrocyte, brain
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK1 (human) phospho-antibody, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS no change compared to control
oxidative_stress no change compared to control
PD98059 decrease
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation
Effect of modification (process):  signaling pathway regulation
Comments:  phosphorylation of this site stimulates interaction with multiple proteins and activates multiple pathways; induces reactive astrogliosis

S59-p - CRYAB (mouse)
Modsite: PSFLRAPsWIDTGLS SwissProt Entrez-Gene
Orthologous residues
CRYAB (human): S59‑p, CRYAB (mouse): S59‑p, CRYAB (rat): S59‑p, CRYAB (rabbit): S59‑p, CRYAB (pig): S59‑p, CRYAB (cow): S59‑p
Characterization
Methods used to characterize site in vivo immunoassay, phospho-antibody
Relevant cell lines - cell types - tissues:  astrocyte, brain
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE P38A (human) phospho-antibody, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cuprizone increase
oxidative_stress increase
LPS increase
SB203580 LPS inhibit treatment-induced increase
SB203580 decrease
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation
Effect of modification (process):  signaling pathway regulation
Comments:  phosphorylation of this site stimulates interaction with multiple proteins and activates multiple pathways; induces reactive astrogliosis