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Home > Curated Information Page > PubMed Id: 28193222
Wang J, et al. (2017) Phosphorylation-dependent regulation of ALDH1A1 by Aurora kinase A: insights on their synergistic relationship in pancreatic cancer. BMC Biol 15, 10 28193222
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T267-p - ALDH1A1 (human)
Modsite: KSNLKRVtLELGGKS SwissProt Entrez-Gene
Orthologous residues
ALDH1A1 (human): T267‑p, ALDH1A1 (mouse): T267‑p, ALDH1A1 (rat): T267‑p, ALDH1A1 (sheep): S267‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Disease tissue studied:  pancreatic cancer, pancreatic carcinoma
Relevant cell lines - cell types - tissues:  BxPC-3 (pancreatic), PANC-1 (pancreatic)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurA (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE AurA (human) co-immunoprecipitation, siRNA inhibition of enzyme, transfection of wild-type enzyme, pharmacological inhibitor of upstream enzyme
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, intracellular localization, protein conformation, protein stabilization, ubiquitination
Effect of modification (process):  carcinogenesis, induced, cell growth, induced, cell motility, induced, transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ALDH1A1 (human) Disrupts electrophoretic visualization
Comments:  inhibits ubiquitination, induces drug resistance, T267 site ALDHA1A1 increases enzymatic activity, oligomers dissociate to the monomeric form

T442-p - ALDH1A1 (human)
Modsite: kDIDKAItISSALQA SwissProt Entrez-Gene
Orthologous residues
ALDH1A1 (human): T442‑p, ALDH1A1 (mouse): T442‑p, ALDH1A1 (rat): T442‑p, ALDH1A1 (sheep): T442‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Disease tissue studied:  pancreatic cancer, pancreatic carcinoma
Relevant cell lines - cell types - tissues:  BxPC-3 (pancreatic), PANC-1 (pancreatic)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurA (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE AurA (human) co-immunoprecipitation, siRNA inhibition of enzyme, transfection of wild-type enzyme, pharmacological inhibitor of upstream enzyme
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, intracellular localization, protein conformation, protein stabilization, ubiquitination
Effect of modification (process):  carcinogenesis, induced, cell growth, induced, cell motility, induced, transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ALDH1A1 (human) Disrupts electrophoretic visualization
Comments:  inhibits ubiquitination, induces drug resistance, T267 site ALDHA1A1 increases enzymatic activity, oligomers dissociate to the monomeric form

T493-p - ALDH1A1 (human)
Modsite: yTEVkTVtVkISQKN SwissProt Entrez-Gene
Orthologous residues
ALDH1A1 (human): T493‑p, ALDH1A1 (mouse): A493‑p, ALDH1A1 (rat): A493‑p, ALDH1A1 (sheep): T493‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Disease tissue studied:  pancreatic cancer, pancreatic carcinoma
Relevant cell lines - cell types - tissues:  BxPC-3 (pancreatic), PANC-1 (pancreatic)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AurA (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE AurA (human) co-immunoprecipitation, siRNA inhibition of enzyme, transfection of wild-type enzyme, pharmacological inhibitor of upstream enzyme
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, intracellular localization, protein conformation, protein stabilization, ubiquitination
Effect of modification (process):  carcinogenesis, induced, cell growth, induced, cell motility, induced, transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ALDH1A1 (human) Disrupts electrophoretic visualization
Comments:  inhibits ubiquitination, induces drug resistance, T267 site ALDHA1A1 increases enzymatic activity, oligomers dissociate to the monomeric form