Marin TL, et al. (2017) AMPK promotes mitochondrial biogenesis and function by phosphorylating the epigenetic factors DNMT1, RBBP7, and HAT1. Sci Signal 10 28143904

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S730-p - DNMT1 iso2 (human) ▲

Modsite: DNIPEMPsPKKMHQG SwissProt Entrez-Gene Orthologous residues DNMT1 (human): S714‑p, DNMT1 iso2 (human): S730‑p, DNMT1 (mouse): S717‑p, DNMT1 (rat): S718‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, western blotting Relevant cell lines - cell types - tissues:  HUVEC (endothelial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE AMPKA1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE AMPKA1 (human) transfection of wild-type enzyme, co-immunoprecipitation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes acadesine increase Downstream Regulation Effect of modification (function):  activity, inhibited, molecular association, regulation, protein conformation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays RBBP7 (human) Induces co-immunoprecipitation Comments:  Mitochondrial biogenesis

S190-p - HAT1 (human) ▲

Modsite: MWFIETAsFIDVDDE SwissProt Entrez-Gene Orthologous residues HAT1 (human): S190‑p, HAT1 (mouse): S187‑p, HAT1 (rat): S190‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, western blotting Relevant cell lines - cell types - tissues:  HUVEC (endothelial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE AMPKA1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE AMPKA1 (human) transfection of wild-type enzyme, co-immunoprecipitation Downstream Regulation Effect of modification (function):  activity, induced, molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays RBBP7 (human) Induces co-immunoprecipitation Comments:  Mitochondrial biogenesis

S314-p - RBBP7 (human) ▲

Modsite: LkLHTFEsHkDEIFQ SwissProt Entrez-Gene Orthologous residues RBBP7 (human): S314‑p, RBBP7 iso2 (human): S358‑p, RBBP7 (mouse): S314‑p, RBBP7 (rat): S314‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, western blotting Relevant cell lines - cell types - tissues:  HUVEC (endothelial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE AMPKA1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE AMPKA1 (human) transfection of wild-type enzyme, co-immunoprecipitation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes acadesine increase Downstream Regulation Comments:  Mitochondrial biogenesis