Curated Information
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Home > Curated Information Page > PubMed Id: 28132841
Willemsen J, et al. (2017) Phosphorylation-Dependent Feedback Inhibition of RIG-I by DAPK1 Identified by Kinome-wide siRNA Screening. Mol Cell 65, 403-415.e8 28132841
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S308-p - DAPK1 (human)
Modsite: ARKKWkQsVRLISLC SwissProt Entrez-Gene
Orthologous residues
DAPK1 (human): S308‑p, DAPK1 iso3 (human): S308‑p, DAPK1 (mouse): S308‑p, DAPK1 (rat): S308‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
double-stranded_RNA decrease

S8-p - DDX58 (human)
Modsite: MTTEQRRsLQAFQDY SwissProt Entrez-Gene
Orthologous residues
DDX58 (human): S8‑p, DDX58 (mouse): N8‑p, DDX58 (duck): S8‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Cellular systems studied:  cell lines
Enzymes shown to modify site in vitro
Type Enzyme
KINASE DAPK1 (human)

S654-p - DDX58 (human)
Modsite: IEGNPkLsFLkPGIL SwissProt Entrez-Gene
Orthologous residues
DDX58 (human): S654‑p, DDX58 (mouse): S655‑p, DDX58 (duck): N655‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Cellular systems studied:  cell lines
Enzymes shown to modify site in vitro
Type Enzyme
KINASE DAPK1 (human)

T667-p - DDX58 (human)
Modsite: ILTGRGktNQNtGMt SwissProt Entrez-Gene
Orthologous residues
DDX58 (human): T667‑p, DDX58 (mouse): T668‑p, DDX58 (duck): R668‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE DAPK1 (human)
Downstream Regulation
Effect of modification (function):  activity, inhibited, molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Disrupts co-immunoprecipitation

T671-p - DDX58 (human)
Modsite: RGktNQNtGMtLPAQ SwissProt Entrez-Gene
Orthologous residues
DDX58 (human): T671‑p, DDX58 (mouse): T672‑p, DDX58 (duck): T672‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Cellular systems studied:  cell lines
Enzymes shown to modify site in vitro
Type Enzyme
KINASE DAPK1 (human)

T674-p - DDX58 (human)
Modsite: tNQNtGMtLPAQkCI SwissProt Entrez-Gene
Orthologous residues
DDX58 (human): T674‑p, DDX58 (mouse): T675‑p, DDX58 (duck): T675‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Cellular systems studied:  cell lines
Enzymes shown to modify site in vitro
Type Enzyme
KINASE DAPK1 (human)

S764-p - DDX58 (human)
Modsite: kEkMMNDsILRLQtW SwissProt Entrez-Gene
Orthologous residues
DDX58 (human): S764‑p, DDX58 (mouse): S765‑p, DDX58 (duck): A765‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Cellular systems studied:  cell lines
Enzymes shown to modify site in vitro
Type Enzyme
KINASE DAPK1 (human)

T770-p - DDX58 (human)
Modsite: DsILRLQtWDEAVFR SwissProt Entrez-Gene
Orthologous residues
DDX58 (human): T770‑p, DDX58 (mouse): T771‑p, DDX58 (duck): K771‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Cellular systems studied:  cell lines
Enzymes shown to modify site in vitro
Type Enzyme
KINASE DAPK1 (human)