Taira J, et al. (2017) Phosphorylation of clustered serine residues in the N-terminus of BPS domain negatively regulates formation of the complex between human Grb14 and insulin receptor. J Biochem 162, 113-122 28130417

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S358-p - GRB14 (human) ▲

Modsite: MHPYQGRsGCSsQSI SwissProt Entrez-Gene Orthologous residues GRB14 (human): S358‑p, GRB14 iso2 (human): S271‑p, GRB14 (mouse): S356‑p, GRB14 iso3 (mouse): ‑, GRB14 (rat): S356‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry (in vitro), mutation of modification site Relevant cell lines - cell types - tissues:  COS7 (fibroblast), S.cerevisiae Cellular systems studied:  cell lines Species studied:  green monkey, yeast Enzymes shown to modify site in vitro:  Type Enzyme KINASE GSK3A (human) KINASE GSK3B (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GSK3A (human) pharmacological inhibitor of upstream enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays INSR (human) Disrupts yeast two-hybrid, plasmon resonance, co-immunoprecipitation

S362-p - GRB14 (human) ▲

Modsite: QGRsGCSsQSIsPMR SwissProt Entrez-Gene Orthologous residues GRB14 (human): S362‑p, GRB14 iso2 (human): S275‑p, GRB14 (mouse): S360‑p, GRB14 iso3 (mouse): ‑, GRB14 (rat): S360‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry (in vitro), mutation of modification site Relevant cell lines - cell types - tissues:  COS7 (fibroblast), S.cerevisiae Cellular systems studied:  cell lines Species studied:  green monkey, yeast Enzymes shown to modify site in vitro:  Type Enzyme KINASE GSK3B (human) KINASE GSK3A (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GSK3A (human) pharmacological inhibitor of upstream enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays INSR (human) Disrupts yeast two-hybrid, plasmon resonance, co-immunoprecipitation

S366-p - GRB14 (human) ▲

Modsite: GCSsQSIsPMRsIsE SwissProt Entrez-Gene Orthologous residues GRB14 (human): S366‑p, GRB14 iso2 (human): S279‑p, GRB14 (mouse): S364‑p, GRB14 iso3 (mouse): ‑, GRB14 (rat): S364‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry (in vitro), mutation of modification site Relevant cell lines - cell types - tissues:  COS7 (fibroblast), S.cerevisiae Cellular systems studied:  cell lines Species studied:  green monkey, yeast Enzymes shown to modify site in vitro:  Type Enzyme KINASE GSK3B (human) KINASE GSK3A (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GSK3A (human) pharmacological inhibitor of upstream enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays INSR (human) Disrupts yeast two-hybrid, plasmon resonance, co-immunoprecipitation

S419-p - GRB14 (human) ▲

Modsite: LRLGTHGsPtAsSQS SwissProt Entrez-Gene Orthologous residues GRB14 (human): S419‑p, GRB14 iso2 (human): S332‑p, GRB14 (mouse): S417‑p, GRB14 iso3 (mouse): ‑, GRB14 (rat): S417‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry (in vitro), mutation of modification site Relevant cell lines - cell types - tissues:  COS7 (fibroblast), S.cerevisiae Cellular systems studied:  cell lines Species studied:  green monkey, yeast Enzymes shown to modify site in vitro:  Type Enzyme KINASE GSK3B (human) KINASE GSK3A (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GSK3A (human) pharmacological inhibitor of upstream enzyme

S423-p - GRB14 (human) ▲

Modsite: THGsPtAsSQSSATN SwissProt Entrez-Gene Orthologous residues GRB14 (human): S423‑p, GRB14 iso2 (human): S336‑p, GRB14 (mouse): P421‑p, GRB14 iso3 (mouse): ‑, GRB14 (rat): P421‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry (in vitro), mutation of modification site Relevant cell lines - cell types - tissues:  COS7 (fibroblast), S.cerevisiae Cellular systems studied:  cell lines Species studied:  green monkey, yeast Enzymes shown to modify site in vitro:  Type Enzyme KINASE GSK3A (human) KINASE GSK3B (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GSK3A (human) pharmacological inhibitor of upstream enzyme