Curated Information
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Home > Curated Information Page > PubMed Id: 28096417
CastaƱeda-Bueno M, et al. (2017) Phosphorylation by PKC and PKA regulate the kinase activity and downstream signaling of WNK4. Proc Natl Acad Sci U S A 114, E879-E886 28096417
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S47-p - WNK4 (mouse)
Modsite: PRRVRRFsGKAEPRP SwissProt Entrez-Gene
Orthologous residues
WNK4 (human): S47‑p, WNK4 (mouse): S47‑p, WNK4 (rat): S47‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  green monkey
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
angiotensin_2 increase
colforsin increase
H-89 decrease
phorbol_ester increase
bisindolylmaleimide decrease

S64-p - WNK4 (mouse)
Modsite: SRPSRRSsVDLGLLS SwissProt Entrez-Gene
Orthologous residues
WNK4 (human): S64‑p, WNK4 (mouse): S64‑p, WNK4 (rat): S64‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  green monkey
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
high-salt diet no change compared to control
furosemide, low-salt diet increase
angiotensin_2 increase
colforsin increase
H-89 decrease
phorbol_ester increase
bisindolylmaleimide decrease
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S332-p - WNK4 (mouse)
Modsite: kRAsFAKsVIGTPEF SwissProt Entrez-Gene
Orthologous residues
WNK4 (human): S335‑p, WNK4 (mouse): S332‑p, WNK4 (rat): S332‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  green monkey
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
colforsin increase
angiotensin_2 increase
bisindolylmaleimide decrease
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S1169-p - WNK4 (mouse)
Modsite: SCRQRRLsKGsFPTS SwissProt Entrez-Gene
Orthologous residues
WNK4 (human): S1190‑p, WNK4 (mouse): S1169‑p, WNK4 (rat): S1169‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  green monkey
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
angiotensin_2 increase
colforsin increase
H-89 decrease
phorbol_ester increase
bisindolylmaleimide decrease

S1180-p - WNK4 (mouse)
Modsite: FPTSRRNsLQRSDLP SwissProt Entrez-Gene
Orthologous residues
WNK4 (human): S1201‑p, WNK4 (mouse): S1180‑p, WNK4 (rat): S1180‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  green monkey
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
angiotensin_2 increase
colforsin increase
H-89 decrease
phorbol_ester increase
bisindolylmaleimide decrease

S1196-p - WNK4 (mouse)
Modsite: PGIMRRNsLSGSSTG SwissProt Entrez-Gene
Orthologous residues
WNK4 (human): S1217‑p, WNK4 (mouse): S1196‑p, WNK4 (rat): S1196‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  green monkey
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
high-salt diet no change compared to control
furosemide, low-salt diet increase
angiotensin_2 increase
colforsin increase
H-89 decrease
phorbol_ester increase
bisindolylmaleimide decrease
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced