Curated Information
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Home > Curated Information Page > PubMed Id: 28073914
Li R, et al. (2017) Serine/Threonine Kinase Unc-51-like Kinase-1 (Ulk1) Phosphorylates the Co-chaperone Cell Division Cycle Protein 37 (Cdc37) and Thereby Disrupts the Stability of Cdc37 Client Proteins. J Biol Chem 292, 2830-2841 28073914
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S127-p - CDC37 (human)
Modsite: skDGFSksMVNtkPE SwissProt Entrez-Gene
Orthologous residues
CDC37 (human): S127‑p, CDC37 (mouse): S128‑p, CDC37 (rat): S128‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  293 (epithelial), HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human

T131-p - CDC37 (human)
Modsite: FSksMVNtkPEktEE SwissProt Entrez-Gene
Orthologous residues
CDC37 (human): T131‑p, CDC37 (mouse): T132‑p, CDC37 (rat): T132‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  293 (epithelial), HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human

S339-p - CDC37 (human)
Modsite: HMQRCIDsGLWVPNs SwissProt Entrez-Gene
Orthologous residues
CDC37 (human): S339‑p, CDC37 (mouse): S340‑p, CDC37 (rat): S340‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  293 (epithelial), HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ULK1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ULK1 (human) phospho-antibody, phosphopeptide analysis, co-immunoprecipitation, transfection of wild-type enzyme
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CDK4 (human) Disrupts ubiquitination, protein degradation co-immunoprecipitation
Src (human) Disrupts ubiquitination, protein degradation co-immunoprecipitation
AurB (human) Disrupts ubiquitination, protein degradation co-immunoprecipitation