Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.6.0.2
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 18931661
Higuchi M, Onishi K, Kikuchi C, Gotoh Y (2008) Scaffolding function of PAK in the PDK1-Akt pathway. Nat Cell Biol 10, 1356-64 18931661
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

T308-p - Akt1 (human)
Modsite: kDGAtMKtFCGtPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
RAC1 (human) increase
PAK1 (rat) inhibit treatment-induced increase inhibits induction by Rac1
PAK2 (human) inhibit treatment-induced increase inhibits induction by Rac1
PAK1 (rat) inhibit treatment-induced increase dominant negative PAK1 inhibits induction by Rac1
serum increase
serum PAK1 (rat) inhibit treatment-induced increase dominant negative Pak1
PAK1 (rat) increase Kinase negative (KN) PAK mutant did inhibt induction of Akt.
Akt1 (human), PAK1 (rat) no change compared to control Akt WT, PAK1 N-SP mutant
PDGF increase
siRNA PDGF PAK2 (human), PAK1 (rat) inhibit treatment-induced increase
Akt2 (human), PAK1 (rat) no change compared to control PAK1 promoted phosphorylation of Akt1 but not Akt2
Akt2 (human), PAK1 (rat) no change compared to control dominant negative PAK1 inhbited phosphorylation of Akt2
PAK1 (rat) no change compared to control Akt S473A with kinase negative mutant showed no effect on AKT T308 induction
PAK1 (rat) augment treatment-induced decrease PAK1 induced translocation to membrane is not sufficient for S308 phosphorylation
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, intracellular localization, molecular association, regulation
Effect of modification (process):  cell motility, altered
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PAK1 (human) Induces molecular association, regulation, intracellular localization cell motility, altered co-immunoprecipitation, in vitro
PDK1 (human) Induces molecular association, regulation co-immunoprecipitation
Comments:  Akt translocation to membrane; complex of PAK1, and PDK1, and Akt facilitating Akt stimulatioon

S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
RAC1 (human) increase
PAK1 (rat) inhibit treatment-induced increase inhibits induction by Rac1
PAK2 (human) inhibit treatment-induced increase inhibits induction by Rac1
PAK1 (rat) inhibit treatment-induced increase dominant negative PAK1 inhibits induction by Rac1
serum increase
serum PAK1 (rat) inhibit treatment-induced increase dominant negative Pak1
PAK1 (rat) increase Kinase negative (KN) PAK mutant did inhibt induction of Akt.
Akt1 (human), PAK1 (rat) no change compared to control Akt WT, PAK1 N-SP mutant
PDGF increase
siRNA PDGF PAK2 (human), PAK1 (rat) inhibit treatment-induced increase
Akt2 (human), PAK1 (rat) increase Akt2, PAK1-kinase negative: Akt1 promoted phosphorylation more effectively than Akt2
Akt2 (human), PAK1 (rat) no change compared to control Akt2, PAK1 N-SP, dominant negative PAK inhibited induction of Akt phosphorylation
PAK1 (rat) increase PAK1 induces translocation of Akt to membrane and S4734 phosphorylation
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, intracellular localization, molecular association, regulation
Effect of modification (process):  cell motility, altered
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PAK1 (human) Induces molecular association, regulation, intracellular localization cell motility, altered co-immunoprecipitation, in vitro
PDK1 (human) Induces molecular association, regulation co-immunoprecipitation
Comments:  Akt translocation to membrane; complex of PAK1, and PDK1, and Akt facilitating Akt stimulatioon

S198-p - PAK1 (rat)
Modsite: PRPEHTKsVYTRsVI SwissProt Entrez-Gene
Orthologous residues
PAK1 (human): S199‑p, PAK1 (mouse): S199‑p, PAK1 (rat): S198‑p

S203-p - PAK1 (rat)
Modsite: TKsVYTRsVIEPLPV SwissProt Entrez-Gene
Orthologous residues
PAK1 (human): S204‑p, PAK1 (mouse): S204‑p, PAK1 (rat): S203‑p