Heath RJ, et al. (2016) RNF166 Determines Recruitment of Adaptor Proteins during Antibacterial Autophagy. Cell Rep 17, 2183-2194 27880896

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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K91-ub - SQSTM1 (human) ▲

Modsite: TMAMSYVkDDIFRIY SwissProt Entrez-Gene Orthologous residues SQSTM1 (human): K91‑ub, SQSTM1 iso2 (human): K7‑ub, SQSTM1 (mouse): K91‑ub, SQSTM1 iso2 (mouse): K91‑ub, SQSTM1 (rat): K89‑ub, SQSTM1 iso2 (rat): K89‑ub, SQSTM1 iso3 (rat): K89‑ub Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme UBIQUITIN LIGASE UBE1 (human) UBIQUITIN LIGASE UBE2D2 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes UBIQUITIN LIGASE RNF166 (human) transfection of wild-type enzyme, co-immunoprecipitation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes RNF166 (human) increase E3-ubiquitin ligase Downstream Regulation Effect of modification (process):  autophagy, induced Comments:  antibacterial autophagy

K189-ub - SQSTM1 (human) ▲

Modsite: srWLRkVkHGHFGWP SwissProt Entrez-Gene Orthologous residues SQSTM1 (human): K189‑ub, SQSTM1 iso2 (human): K105‑ub, SQSTM1 (mouse): K189‑ub, SQSTM1 iso2 (mouse): K189‑ub, SQSTM1 (rat): K186‑ub, SQSTM1 iso2 (rat): K186‑ub, SQSTM1 iso3 (rat): K186‑ub Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme UBIQUITIN LIGASE UBE1 (human) UBIQUITIN LIGASE UBE2D2 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes UBIQUITIN LIGASE RNF166 (human) transfection of wild-type enzyme, co-immunoprecipitation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes RNF166 (human) increase E3-ubiquitin ligase Downstream Regulation Effect of modification (process):  autophagy, induced Comments:  antibacterial autophagy