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Home > Curated Information Page > PubMed Id: 27895153
Ishida N, et al. (2017) Ubiquitylation of Ku80 by RNF126 Promotes Completion of Nonhomologous End Joining-Mediated DNA Repair. Mol Cell Biol 37 27895153
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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K36-ub - Ku80 (human)
Modsite: SPFEQAKkVItMFVQ SwissProt Entrez-Gene
Orthologous residues
Ku80 (human): K36‑ub, Ku80 (mouse): K36‑ub
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer, lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast), HEK293T (epithelial), NCI-H1299 (pulmonary), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  hamster, human
Enzymes shown to modify site in vitro
Type Enzyme
UBIQUITIN LIGASE RNF126 (human)
UBIQUITIN LIGASE UBE2D3 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE RNF126 (human) transfection of inactive enzyme, transfection of wild-type enzyme, siRNA inhibition of enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  DNA repair, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Disrupts

K144-ub - Ku80 (human)
Modsite: DLSSrFSksQLDIII SwissProt Entrez-Gene
Orthologous residues
Ku80 (human): K144‑ub, Ku80 (mouse): Q144‑ub
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer, lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast), HEK293T (epithelial), NCI-H1299 (pulmonary), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  hamster, human
Enzymes shown to modify site in vitro
Type Enzyme
UBIQUITIN LIGASE UBE2D3 (human)
UBIQUITIN LIGASE RNF126 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE RNF126 (human) transfection of inactive enzyme, transfection of wild-type enzyme, siRNA inhibition of enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  DNA repair, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Disrupts

K155-ub - Ku80 (human)
Modsite: DIIIHSLkkCDISLQ SwissProt Entrez-Gene
Orthologous residues
Ku80 (human): K155‑ub, Ku80 (mouse): K155‑ub
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer, lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast), HEK293T (epithelial), NCI-H1299 (pulmonary), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  hamster, human
Enzymes shown to modify site in vitro
Type Enzyme
UBIQUITIN LIGASE UBE2D3 (human)
UBIQUITIN LIGASE RNF126 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE RNF126 (human) transfection of inactive enzyme, transfection of wild-type enzyme, siRNA inhibition of enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  DNA repair, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Disrupts

K156-ub - Ku80 (human)
Modsite: IIIHSLkkCDISLQF SwissProt Entrez-Gene
Orthologous residues
Ku80 (human): K156‑ub, Ku80 (mouse): K156‑ub
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer, lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast), HEK293T (epithelial), NCI-H1299 (pulmonary), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  hamster, human
Enzymes shown to modify site in vitro
Type Enzyme
UBIQUITIN LIGASE UBE2D3 (human)
UBIQUITIN LIGASE RNF126 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE RNF126 (human) transfection of inactive enzyme, transfection of wild-type enzyme, siRNA inhibition of enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  DNA repair, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Disrupts

K195-ub - Ku80 (human)
Modsite: HGPsFPLkGItEQQk SwissProt Entrez-Gene
Orthologous residues
Ku80 (human): K195‑ub, Ku80 (mouse): K195‑ub
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer, lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast), HEK293T (epithelial), NCI-H1299 (pulmonary), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  hamster, human
Enzymes shown to modify site in vitro
Type Enzyme
UBIQUITIN LIGASE UBE2D3 (human)
UBIQUITIN LIGASE RNF126 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE RNF126 (human) transfection of inactive enzyme, transfection of wild-type enzyme, siRNA inhibition of enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  DNA repair, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Disrupts

K202-ub - Ku80 (human)
Modsite: kGItEQQkEGLEIVk SwissProt Entrez-Gene
Orthologous residues
Ku80 (human): K202‑ub, Ku80 (mouse): K202‑ub
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer, lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast), HEK293T (epithelial), NCI-H1299 (pulmonary), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  hamster, human
Enzymes shown to modify site in vitro
Type Enzyme
UBIQUITIN LIGASE UBE2D3 (human)
UBIQUITIN LIGASE RNF126 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE RNF126 (human) transfection of inactive enzyme, transfection of wild-type enzyme, siRNA inhibition of enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  DNA repair, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Disrupts

K282-ub - Ku80 (human)
Modsite: TWtVVDAkTLkKEDI SwissProt Entrez-Gene
Orthologous residues
Ku80 (human): K282‑ub, Ku80 (mouse): R282‑ub
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer, lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast), HEK293T (epithelial), NCI-H1299 (pulmonary), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  hamster, human
Enzymes shown to modify site in vitro
Type Enzyme
UBIQUITIN LIGASE RNF126 (human)
UBIQUITIN LIGASE UBE2D3 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE RNF126 (human) transfection of inactive enzyme, transfection of wild-type enzyme, siRNA inhibition of enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  DNA repair, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Disrupts

K285-ub - Ku80 (human)
Modsite: VVDAkTLkKEDIQkE SwissProt Entrez-Gene
Orthologous residues
Ku80 (human): K285‑ub, Ku80 (mouse): K285‑ub
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer, lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast), HEK293T (epithelial), NCI-H1299 (pulmonary), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  hamster, human
Enzymes shown to modify site in vitro
Type Enzyme
UBIQUITIN LIGASE RNF126 (human)
UBIQUITIN LIGASE UBE2D3 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE RNF126 (human) transfection of inactive enzyme, transfection of wild-type enzyme, siRNA inhibition of enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  DNA repair, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Disrupts

K334-ub - Ku80 (human)
Modsite: DEEQMkYksEGkCFs SwissProt Entrez-Gene
Orthologous residues
Ku80 (human): K334‑ub, Ku80 (mouse): K334‑ub
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer, lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast), HEK293T (epithelial), NCI-H1299 (pulmonary), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  hamster, human
Enzymes shown to modify site in vitro
Type Enzyme
UBIQUITIN LIGASE UBE2D3 (human)
UBIQUITIN LIGASE RNF126 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE RNF126 (human) transfection of inactive enzyme, transfection of wild-type enzyme, siRNA inhibition of enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  DNA repair, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Disrupts

K439-ub - Ku80 (human)
Modsite: QYMFssLkNSKkYAP SwissProt Entrez-Gene
Orthologous residues
Ku80 (human): K439‑ub, Ku80 (mouse): K439‑ub
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer, lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast), HEK293T (epithelial), NCI-H1299 (pulmonary), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  hamster, human
Enzymes shown to modify site in vitro
Type Enzyme
UBIQUITIN LIGASE RNF126 (human)
UBIQUITIN LIGASE UBE2D3 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE RNF126 (human) transfection of inactive enzyme, transfection of wild-type enzyme, siRNA inhibition of enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  DNA repair, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Disrupts

K443-ub - Ku80 (human)
Modsite: ssLkNSKkYAPTEAQ SwissProt Entrez-Gene
Orthologous residues
Ku80 (human): K443‑ub, Ku80 (mouse): K443‑ub
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer, lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast), HEK293T (epithelial), NCI-H1299 (pulmonary), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  hamster, human
Enzymes shown to modify site in vitro
Type Enzyme
UBIQUITIN LIGASE UBE2D3 (human)
UBIQUITIN LIGASE RNF126 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE RNF126 (human) transfection of inactive enzyme, transfection of wild-type enzyme, siRNA inhibition of enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  DNA repair, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Disrupts

K465-ub - Ku80 (human)
Modsite: IDSMSLAkkDEkTDt SwissProt Entrez-Gene
Orthologous residues
Ku80 (human): K465‑ub, Ku80 (mouse): K465‑ub
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer, lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast), HEK293T (epithelial), NCI-H1299 (pulmonary), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  hamster, human
Enzymes shown to modify site in vitro
Type Enzyme
UBIQUITIN LIGASE RNF126 (human)
UBIQUITIN LIGASE UBE2D3 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE RNF126 (human) transfection of inactive enzyme, transfection of wild-type enzyme, siRNA inhibition of enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  DNA repair, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Disrupts

K469-ub - Ku80 (human)
Modsite: SLAkkDEkTDtLEDL SwissProt Entrez-Gene
Orthologous residues
Ku80 (human): K469‑ub, Ku80 (mouse): E469‑ub
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer, lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast), HEK293T (epithelial), NCI-H1299 (pulmonary), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  hamster, human
Enzymes shown to modify site in vitro
Type Enzyme
UBIQUITIN LIGASE UBE2D3 (human)
UBIQUITIN LIGASE RNF126 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE RNF126 (human) transfection of inactive enzyme, transfection of wild-type enzyme, siRNA inhibition of enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  DNA repair, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Disrupts

K525-ub - Ku80 (human)
Modsite: PPAEVttksQIPLsk SwissProt Entrez-Gene
Orthologous residues
Ku80 (human): K525‑ub, Ku80 (mouse): K525‑ub
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer, lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast), HEK293T (epithelial), NCI-H1299 (pulmonary), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  hamster, human
Enzymes shown to modify site in vitro
Type Enzyme
UBIQUITIN LIGASE RNF126 (human)
UBIQUITIN LIGASE UBE2D3 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE RNF126 (human) transfection of inactive enzyme, transfection of wild-type enzyme, siRNA inhibition of enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  DNA repair, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Disrupts

K532-ub - Ku80 (human)
Modsite: ksQIPLskIktLFPL SwissProt Entrez-Gene
Orthologous residues
Ku80 (human): K532‑ub, Ku80 (mouse): K532‑ub
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer, lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast), HEK293T (epithelial), NCI-H1299 (pulmonary), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  hamster, human
Enzymes shown to modify site in vitro
Type Enzyme
UBIQUITIN LIGASE RNF126 (human)
UBIQUITIN LIGASE UBE2D3 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE RNF126 (human) transfection of inactive enzyme, transfection of wild-type enzyme, siRNA inhibition of enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  DNA repair, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Disrupts

K534-ub - Ku80 (human)
Modsite: QIPLskIktLFPLIE SwissProt Entrez-Gene
Orthologous residues
Ku80 (human): K534‑ub, Ku80 (mouse): K534‑ub
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer, lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast), HEK293T (epithelial), NCI-H1299 (pulmonary), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  hamster, human
Enzymes shown to modify site in vitro
Type Enzyme
UBIQUITIN LIGASE RNF126 (human)
UBIQUITIN LIGASE UBE2D3 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE RNF126 (human) transfection of inactive enzyme, transfection of wild-type enzyme, siRNA inhibition of enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  DNA repair, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Disrupts

K543-ub - Ku80 (human)
Modsite: LFPLIEAkkkDQVTA SwissProt Entrez-Gene
Orthologous residues
Ku80 (human): K543‑ub, Ku80 (mouse): K544‑ub
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer, lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast), HEK293T (epithelial), NCI-H1299 (pulmonary), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  hamster, human
Enzymes shown to modify site in vitro
Type Enzyme
UBIQUITIN LIGASE RNF126 (human)
UBIQUITIN LIGASE UBE2D3 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE RNF126 (human) transfection of inactive enzyme, transfection of wild-type enzyme, siRNA inhibition of enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  DNA repair, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Disrupts

K544-ub - Ku80 (human)
Modsite: FPLIEAkkkDQVTAQ SwissProt Entrez-Gene
Orthologous residues
Ku80 (human): K544‑ub, Ku80 (mouse): K545‑ub
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer, lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast), HEK293T (epithelial), NCI-H1299 (pulmonary), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  hamster, human
Enzymes shown to modify site in vitro
Type Enzyme
UBIQUITIN LIGASE RNF126 (human)
UBIQUITIN LIGASE UBE2D3 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE RNF126 (human) transfection of inactive enzyme, transfection of wild-type enzyme, siRNA inhibition of enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  DNA repair, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Disrupts

K545-ub - Ku80 (human)
Modsite: PLIEAkkkDQVTAQE SwissProt Entrez-Gene
Orthologous residues
Ku80 (human): K545‑ub, Ku80 (mouse): K546‑ub
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer, lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  CHO-IR (fibroblast), HEK293T (epithelial), NCI-H1299 (pulmonary), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  hamster, human
Enzymes shown to modify site in vitro
Type Enzyme
UBIQUITIN LIGASE RNF126 (human)
UBIQUITIN LIGASE UBE2D3 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE RNF126 (human) transfection of inactive enzyme, transfection of wild-type enzyme, siRNA inhibition of enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  DNA repair, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Disrupts