Curated Information
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Home > Curated Information Page > PubMed Id: 15448698
Zhou BP, et al. (2004) Dual regulation of Snail by GSK-3beta-mediated phosphorylation in control of epithelial-mesenchymal transition. Nat Cell Biol 6, 931-40 15448698
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 increase Snail-MCF-7, Snail HEK293
EGF increase
IGF-1 EGF increase
IGF-1 increase Snail-MCF-7, Snail-HEK 293
wortmannin IGF-1 inhibit treatment-induced increase
PD98059 IGF-1 no effect upon treatment-induced increase
U0126 IGF-1 augment treatment-induced increase

T202-p - ERK1 (human)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 increase Snail-MCF-7, Snail HEK293
EGF increase
IGF-1 EGF increase
IGF-1 no change compared to control
wortmannin no change compared to control
PD98059 decrease
U0126 decrease

Y204-p - ERK1 (human)
Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 increase Snail-MCF-7, Snail HEK293
EGF increase
IGF-1 EGF increase
IGF-1 no change compared to control
wortmannin no change compared to control
PD98059 decrease
U0126 decrease

T185-p - ERK2 (human)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 increase Snail-MCF-7, Snail HEK293
EGF increase
IGF-1 EGF increase
IGF-1 no change compared to control
wortmannin no change compared to control
PD98059 decrease
U0126 decrease

Y187-p - ERK2 (human)
Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 increase Snail-MCF-7, Snail HEK293
EGF increase
IGF-1 EGF increase
IGF-1 no change compared to control
wortmannin no change compared to control
PD98059 decrease
U0126 decrease

S9-p - GSK3B (human)
Modsite: SGRPRttsFAEsCkP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 increase Snail-MCF-7, Snail HEK293
EGF increase
IGF-1 EGF increase
IGF-1 increase
wortmannin IGF-1 inhibit treatment-induced increase
PD98059 IGF-1 inhibit treatment-induced increase
U0126 IGF-1 inhibit treatment-induced increase
MG132 IGF-1 no effect upon treatment-induced increase

S96-p - Snail1 (human)
Modsite: TsLsDEDsGKGsQPP SwissProt Entrez-Gene
Orthologous residues
Snail1 (human): S96‑p, Snail1 (mouse): S96‑p
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) transfection of wild-type enzyme, mutation in upstream enzyme recognition motif, transfection of constitutively active enzyme, transfection of inactive enzyme, co-immunoprecipitation, pharmacological inhibitor of upstream enzyme
Downstream Regulation
Effect of modification (function):  protein degradation, ubiquitination
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
FBW1B (human) Induces protein degradation, ubiquitination co-immunoprecipitation

S100-p - Snail1 (human)
Modsite: DEDsGKGsQPPsPPs SwissProt Entrez-Gene
Orthologous residues
Snail1 (human): S100‑p, Snail1 (mouse): S100‑p
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) transfection of wild-type enzyme, mutation in upstream enzyme recognition motif, transfection of constitutively active enzyme, transfection of inactive enzyme, co-immunoprecipitation, pharmacological inhibitor of upstream enzyme
Downstream Regulation
Effect of modification (function):  protein degradation, ubiquitination
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
FBW1B (human) Induces protein degradation, ubiquitination co-immunoprecipitation

S107-p - Snail1 (human)
Modsite: sQPPsPPsPAPssFS SwissProt Entrez-Gene
Orthologous residues
Snail1 (human): S107‑p, Snail1 (mouse): S107‑p
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) transfection of wild-type enzyme, mutation in upstream enzyme recognition motif, transfection of constitutively active enzyme, transfection of inactive enzyme, co-immunoprecipitation, pharmacological inhibitor of upstream enzyme
Downstream Regulation
Effect of modification (function):  intracellular localization

S111-p - Snail1 (human)
Modsite: sPPsPAPssFSsTSV SwissProt Entrez-Gene
Orthologous residues
Snail1 (human): S111‑p, Snail1 (mouse): S111‑p
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) transfection of wild-type enzyme, mutation in upstream enzyme recognition motif, transfection of constitutively active enzyme, transfection of inactive enzyme, co-immunoprecipitation, pharmacological inhibitor of upstream enzyme
Downstream Regulation
Effect of modification (function):  intracellular localization

S115-p - Snail1 (human)
Modsite: PAPssFSsTSVsSLE SwissProt Entrez-Gene
Orthologous residues
Snail1 (human): S115‑p, Snail1 (mouse): S115‑p
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) transfection of wild-type enzyme, mutation in upstream enzyme recognition motif, transfection of constitutively active enzyme, transfection of inactive enzyme, co-immunoprecipitation, pharmacological inhibitor of upstream enzyme
Downstream Regulation
Effect of modification (function):  intracellular localization

S119-p - Snail1 (human)
Modsite: sFSsTSVsSLEAEAY SwissProt Entrez-Gene
Orthologous residues
Snail1 (human): S119‑p, Snail1 (mouse): S119‑p
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) transfection of wild-type enzyme, mutation in upstream enzyme recognition motif, transfection of constitutively active enzyme, transfection of inactive enzyme, co-immunoprecipitation, pharmacological inhibitor of upstream enzyme
Downstream Regulation
Effect of modification (function):  intracellular localization