Curated Information
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Home > Curated Information Page > PubMed Id: 15772123
Mehebik N, et al. (2005) Leptin-induced nitric oxide production in white adipocytes is mediated through PKA and MAP kinase activation. Am J Physiol Cell Physiol 289, C379-87 15772123
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T494-p - eNOS (rat)
Modsite: TGITRKKtFKEVANA SwissProt Entrez-Gene
Orthologous residues
eNOS (human): T495‑p, eNOS (mouse): T494‑p, eNOS (rat): T494‑p, eNOS (rabbit): T501‑p, eNOS (pig): T497‑p, eNOS (cow): T497‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  adipocyte-adipose tissue
Cellular systems studied:  primary cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
leptin increase
H-89 leptin inhibit treatment-induced increase
U0126 leptin inhibit treatment-induced increase
cAMP_analog increase
H-89 cAMP_analog inhibit treatment-induced increase
U0126 cAMP_analog no effect upon treatment-induced increase
okadaic_acid increase
tautomycin increase
leptin tautomycin augment treatment-induced increase

S1176-p - eNOS (rat)
Modsite: TSRIRtQsFsLQERQ SwissProt Entrez-Gene
Orthologous residues
eNOS (human): S1177‑p, eNOS (mouse): S1176‑p, eNOS (rat): S1176‑p, eNOS (rabbit): S1183‑p, eNOS (pig): S1179‑p, eNOS (cow): S1179‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  adipocyte-adipose tissue
Cellular systems studied:  primary cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
leptin increase
H-89 leptin inhibit treatment-induced increase
U0126 leptin inhibit treatment-induced increase
cAMP_analog increase
H-89 cAMP_analog inhibit treatment-induced increase
U0126 cAMP_analog no effect upon treatment-induced increase
okadaic_acid increase
tautomycin increase
leptin tautomycin inhibit treatment-induced increase

T203-p - ERK1 (rat)
Modsite: HDHTGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  adipocyte-adipose tissue
Cellular systems studied:  primary cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
leptin increase
H-89, leptin leptin no effect upon treatment-induced increase
U0126 leptin inhibit treatment-induced increase

Y205-p - ERK1 (rat)
Modsite: HTGFLtEyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  adipocyte-adipose tissue
Cellular systems studied:  primary cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
leptin increase
H-89, leptin leptin no effect upon treatment-induced increase
U0126 leptin inhibit treatment-induced increase