Medunjanin S, et al. (2016) GSK-3β controls NF-kappaB activity via IKKγ/NEMO. Sci Rep 6, 38553 27929056

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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S8-p - IKKG (human) ▲

Modsite: MNRHLWKsQLCEMVQ SwissProt Entrez-Gene Orthologous residues IKKG (human): S8‑p, IKKG iso2 (human): S76‑p, IKKG (mouse): N8‑p, IKKG (rat): N8‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast), MCF-7 (breast cell) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE GSK3B (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GSK3B (human) co-immunoprecipitation, transfection of constitutively active enzyme, transfection of wild-type enzyme Downstream Regulation Effect of modification (function):  protein stabilization Effect of modification (process):  transcription, induced

S17-p - IKKG (human) ▲

Modsite: LCEMVQPsGGPAADQ SwissProt Entrez-Gene Orthologous residues IKKG (human): S17‑p, IKKG iso2 (human): S85‑p, IKKG (mouse): S17‑p, IKKG (rat): S17‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast), MCF-7 (breast cell) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE GSK3B (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GSK3B (human) co-immunoprecipitation, transfection of constitutively active enzyme, transfection of wild-type enzyme Downstream Regulation Effect of modification (function):  protein stabilization Effect of modification (process):  transcription, induced

S31-p - IKKG (human) ▲

Modsite: QDVLGEEsPLGKPAM SwissProt Entrez-Gene Orthologous residues IKKG (human): S31‑p, IKKG iso2 (human): S99‑p, IKKG (mouse): S31‑p, IKKG (rat): S31‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast), MCF-7 (breast cell) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE GSK3B (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GSK3B (human) co-immunoprecipitation, transfection of constitutively active enzyme, transfection of wild-type enzyme Downstream Regulation Effect of modification (function):  protein stabilization Effect of modification (process):  transcription, induced

S43-p - IKKG (human) ▲

Modsite: PAMLHLPsEQGAPEt SwissProt Entrez-Gene Orthologous residues IKKG (human): S43‑p, IKKG iso2 (human): S111‑p, IKKG (mouse): S43‑p, IKKG (rat): S43‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast), MCF-7 (breast cell) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE GSK3B (human)