Curated Information
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Home > Curated Information Page > PubMed Id: 19004823
Wu Y, Evers BM, Zhou BP (2009) Small C-terminal Domain Phosphatase Enhances Snail Activity through Dephosphorylation. J Biol Chem 284, 640-8 19004823
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF, IGF-1 increase
wortmannin EGF, IGF-1 inhibit treatment-induced increase

S9-p - GSK3B (human)
Modsite: SGRPRttsFAEsCkP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
wortmannin decrease

S96-p - Snail1 (human)
Modsite: TsLsDEDsGKGsQPP SwissProt Entrez-Gene
Orthologous residues
Snail1 (human): S96‑p, Snail1 (mouse): S96‑p
Characterization
Methods used to characterize site in vivo western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), MCF-7 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE CTDSP1 (human)
KINASE GSK3B (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE CTDSP1 (human) mutation in upstream enzyme recognition motif, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  intracellular localization, protein degradation
Effect of modification (process):  cell motility, altered, transcription, altered
Comments:  Localization mediated by S107, S111, S115 and S119.

S100-p - Snail1 (human)
Modsite: DEDsGKGsQPPsPPs SwissProt Entrez-Gene
Orthologous residues
Snail1 (human): S100‑p, Snail1 (mouse): S100‑p
Characterization
Methods used to characterize site in vivo western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), MCF-7 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE CTDSP1 (human)
KINASE GSK3B (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE CTDSP1 (human) mutation in upstream enzyme recognition motif, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  intracellular localization, protein degradation
Effect of modification (process):  cell motility, altered, transcription, altered
Comments:  Localization mediated by S107, S111, S115 and S119.

S107-p - Snail1 (human)
Modsite: sQPPsPPsPAPssFS SwissProt Entrez-Gene
Orthologous residues
Snail1 (human): S107‑p, Snail1 (mouse): S107‑p
Characterization
Methods used to characterize site in vivo western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), MCF-7 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE GSK3B (human)
PHOSPHATASE CTDSP1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE CTDSP1 (human) mutation in upstream enzyme recognition motif, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  intracellular localization
Effect of modification (process):  cell motility, altered, transcription, altered
Comments:  Localization mediated by S107, S111, S115 and S119.

S111-p - Snail1 (human)
Modsite: sPPsPAPssFSsTSV SwissProt Entrez-Gene
Orthologous residues
Snail1 (human): S111‑p, Snail1 (mouse): S111‑p
Characterization
Methods used to characterize site in vivo western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), MCF-7 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE CTDSP1 (human)
KINASE GSK3B (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE CTDSP1 (human) mutation in upstream enzyme recognition motif, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  intracellular localization
Effect of modification (process):  cell motility, altered, transcription, altered
Comments:  Localization mediated by S107, S111, S115 and S119.

S115-p - Snail1 (human)
Modsite: PAPssFSsTSVsSLE SwissProt Entrez-Gene
Orthologous residues
Snail1 (human): S115‑p, Snail1 (mouse): S115‑p
Characterization
Methods used to characterize site in vivo western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), MCF-7 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE CTDSP1 (human)
KINASE GSK3B (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE CTDSP1 (human) mutation in upstream enzyme recognition motif, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  intracellular localization
Effect of modification (process):  cell motility, altered, transcription, altered
Comments:  Localization mediated by S107, S111, S115 and S119.

S119-p - Snail1 (human)
Modsite: sFSsTSVsSLEAEAY SwissProt Entrez-Gene
Orthologous residues
Snail1 (human): S119‑p, Snail1 (mouse): S119‑p
Characterization
Methods used to characterize site in vivo western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), MCF-7 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE CTDSP1 (human)
KINASE GSK3B (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE CTDSP1 (human) mutation in upstream enzyme recognition motif, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  intracellular localization
Effect of modification (process):  cell motility, altered, transcription, altered
Comments:  Localization mediated by S107, S111, S115 and S119.