Curated Information
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Home > Curated Information Page > PubMed Id: 28003362
Takahashi M, Li Y, Dillon TJ, Stork PJ (2017) Phosphorylation of Rap1 by cAMP-dependent Protein Kinase (PKA) Creates a Binding Site for KSR to Sustain ERK Activation by cAMP. J Biol Chem 292, 1449-1461 28003362
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S297-p - KSR (mouse)
Modsite: PTLTRsKsHESQLGN SwissProt Entrez-Gene
Orthologous residues
KSR (human): S311‑p, KSR iso2 (human): S311‑p, KSR iso4 (human): S174‑p, KSR (mouse): S297‑p, KSR (rat):
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 gamma (human) Induces co-immunoprecipitation
Comments:  14-3-3 binding site linking KSR to phosphorylated Rap1

S392-p - KSR (mouse)
Modsite: ARLRRtEsVPsDINN SwissProt Entrez-Gene
Orthologous residues
KSR (human): S406‑p, KSR iso2 (human): S406‑p, KSR iso4 (human): S269‑p, KSR (mouse): S392‑p, KSR (rat): S40‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 gamma (human) Induces co-immunoprecipitation
Comments:  14-3-3 binding site linking KSR to phosphorylated Rap1

S838-p - KSR (mouse)
Modsite: PKLNRRLsHPGHFWK SwissProt Entrez-Gene
Orthologous residues
KSR (human): S888‑p, KSR iso2 (human): S866‑p, KSR iso4 (human): S751‑p, KSR (mouse): S838‑p, KSR (rat): S485‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S179-p - RAP1B (cow)
Modsite: PGKARKKssCQLL__ SwissProt Entrez-Gene
Orthologous residues
RAP1B (human): S179‑p, RAP1B (mouse): S179‑p, RAP1B (rat): S179‑p, RAP1B (cow): S179‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S180-p - RAP1B (cow)
Modsite: GKARKKssCQLL___ SwissProt Entrez-Gene
Orthologous residues
RAP1B (human): S180‑p, RAP1B (mouse): S180‑p, RAP1B (rat): S180‑p, RAP1B (cow): S180‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse