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Home > Curated Information Page > PubMed Id: 27903966
Booth L, et al. (2017) PDE5 inhibitors enhance the lethality of pemetrexed through inhibition of multiple chaperone proteins and via the actions of cyclic GMP and nitric oxide. Oncotarget 8, 1449-1468 27903966
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T308-p - Akt1 (human)
Modsite: kDGAtMKtFCGtPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma, non-small cell large cell lung carcinoma
Relevant cell lines - cell types - tissues:  A549 (pulmonary), NCI-H1975 (pulmonary), NCI-H460 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
pemetrexed, sildenafil decrease

S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma, non-small cell large cell lung carcinoma
Relevant cell lines - cell types - tissues:  A549 (pulmonary), NCI-H1975 (pulmonary), NCI-H460 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human

S355-p - ATG13 (human)
Modsite: DTETVSNssEGRAsP SwissProt Entrez-Gene
Orthologous residues
ATG13 (human): S355‑p, ATG13 (mouse): S354‑p, ATG13 (rat): S317‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma, non-small cell large cell lung carcinoma
Relevant cell lines - cell types - tissues:  A549 (pulmonary), NCI-H1975 (pulmonary), NCI-H460 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
pemetrexed, sildenafil increase

S2448-p - mTOR (human)
Modsite: RsRtRtDsysAGQsV SwissProt Entrez-Gene
Orthologous residues
mTOR (human): S2448‑p, mTOR (mouse): S2448‑p, mTOR (rat): S2448‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma, non-small cell large cell lung carcinoma
Relevant cell lines - cell types - tissues:  A549 (pulmonary), NCI-H1975 (pulmonary), NCI-H460 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
pemetrexed, sildenafil decrease

S2481-p - mTOR (human)
Modsite: tVPEsIHsFIGDGLV SwissProt Entrez-Gene
Orthologous residues
mTOR (human): S2481‑p, mTOR (mouse): S2481‑p, mTOR (rat): S2481‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma, non-small cell large cell lung carcinoma
Relevant cell lines - cell types - tissues:  A549 (pulmonary), NCI-H1975 (pulmonary), NCI-H460 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
pemetrexed, sildenafil decrease

T412-p - p70S6K (human)
Modsite: NQVFLGFtyVAPsVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma, non-small cell large cell lung carcinoma
Relevant cell lines - cell types - tissues:  A549 (pulmonary), NCI-H1975 (pulmonary), NCI-H460 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
pemetrexed, sildenafil decrease

S792-p - Raptor (human)
Modsite: DKMRRASsYSsLNSL SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S792‑p, Raptor (mouse): S792‑p, Raptor (rat): S792‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma, non-small cell large cell lung carcinoma
Relevant cell lines - cell types - tissues:  A549 (pulmonary), NCI-H1975 (pulmonary), NCI-H460 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
pemetrexed, sildenafil increase

T1462-p - TSC2 (human)
Modsite: GLRPRGytISDsAPs SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): T1462‑p, TSC2 iso2 (human): T1419‑p, TSC2 iso3 (human): T1418‑p, TSC2 iso4 (human): T1439‑p, TSC2 (mouse): T1465‑p, TSC2 iso6 (mouse): T1443‑p, TSC2 (rat): T1466‑p, TSC2 iso2 (rat): T1423‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma, non-small cell large cell lung carcinoma
Relevant cell lines - cell types - tissues:  A549 (pulmonary), NCI-H1975 (pulmonary), NCI-H460 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
pemetrexed, sildenafil increase

S317-p - ULK1 (human)
Modsite: SHLASPPsLGEMQQL SwissProt Entrez-Gene
Orthologous residues
ULK1 (human): S317‑p, ULK1 (mouse): S317‑p, ULK1 (rat): S317‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma, non-small cell large cell lung carcinoma
Relevant cell lines - cell types - tissues:  A549 (pulmonary), NCI-H1975 (pulmonary), NCI-H460 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human

S758-p - ULK1 (human)
Modsite: PVVFtVGsPPsGStP SwissProt Entrez-Gene
Orthologous residues
ULK1 (human): S758‑p, ULK1 (mouse): S757‑p, ULK1 (rat): S757‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer, non-small cell lung adenocarcinoma, non-small cell large cell lung carcinoma
Relevant cell lines - cell types - tissues:  A549 (pulmonary), NCI-H1975 (pulmonary), NCI-H460 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
pemetrexed, sildenafil decrease