Curated Information
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Home > Curated Information Page > PubMed Id: 19107194
Sasaki T, et al. (2008) Phosphorylation regulates SIRT1 function. PLoS One 3, e4020 19107194
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S14-p - SIRT1 (human)
Modsite: LALQPGGsPsAAGAD SwissProt Entrez-Gene
Orthologous residues
SIRT1 (human): S14‑p, SIRT1 (mouse): S14‑p, SIRT1 (rat): S14‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  ES (stem)
Cellular systems studied:  cell lines
Species studied:  human

S26-p - SIRT1 (human)
Modsite: GADREAAssPAGEPL SwissProt Entrez-Gene
Orthologous residues
SIRT1 (human): S26‑p, SIRT1 (mouse): S25‑p, SIRT1 (rat): S25‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  ES (stem)
Cellular systems studied:  cell lines
Species studied:  human

S27-p - SIRT1 (human)
Modsite: ADREAAssPAGEPLR SwissProt Entrez-Gene
Orthologous residues
SIRT1 (human): S27‑p, SIRT1 (mouse): Q26‑p, SIRT1 (rat): Q26‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  ES (stem)
Cellular systems studied:  cell lines
Species studied:  human

S47-p - SIRT1 (human)
Modsite: DGPGLERsPGEPGGA SwissProt Entrez-Gene
Orthologous residues
SIRT1 (human): S47‑p, SIRT1 (mouse): S46‑p, SIRT1 (rat): S46‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  ES (stem)
Cellular systems studied:  cell lines
Species studied:  human

S159-p - SIRT1 (human)
Modsite: IITNGFHsCEsDEED SwissProt Entrez-Gene
Orthologous residues
SIRT1 (human): S159‑p, SIRT1 (mouse): S151‑p, SIRT1 (rat): S153‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  ES (stem)
Cellular systems studied:  cell lines
Species studied:  human

S162-p - SIRT1 (human)
Modsite: NGFHsCEsDEEDRAS SwissProt Entrez-Gene
Orthologous residues
SIRT1 (human): S162‑p, SIRT1 (mouse): S154‑p, SIRT1 (rat): S156‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  ES (stem)
Cellular systems studied:  cell lines
Species studied:  human

S172-p - SIRT1 (human)
Modsite: EDRASHAssSDWTPR SwissProt Entrez-Gene
Orthologous residues
SIRT1 (human): S172‑p, SIRT1 (mouse): S164‑p, SIRT1 (rat): S166‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  ES (stem)
Cellular systems studied:  cell lines
Species studied:  human

S173-p - SIRT1 (human)
Modsite: DRASHAssSDWTPRP SwissProt Entrez-Gene
Orthologous residues
SIRT1 (human): S173‑p, SIRT1 (mouse): S165‑p, SIRT1 (rat): S167‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  ES (stem)
Cellular systems studied:  cell lines
Species studied:  human

T530-p - SIRT1 (human)
Modsite: YLSELPPtPLHVsED SwissProt Entrez-Gene
Orthologous residues
SIRT1 (human): T530‑p, SIRT1 (mouse): T522‑p, SIRT1 (rat): T524‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  ES (stem), HEK293T (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Downstream Regulation
Effect of modification (function):  acetylation, enzymatic activity, induced
Effect of modification (process):  cell cycle regulation

S535-p - SIRT1 (human)
Modsite: PPtPLHVsEDsssPE SwissProt Entrez-Gene
Orthologous residues
SIRT1 (human): S535‑p, SIRT1 (mouse): S527‑p, SIRT1 (rat): S529‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  ES (stem)
Cellular systems studied:  cell lines
Species studied:  human

S540-p - SIRT1 (human)
Modsite: HVsEDsssPERtsPP SwissProt Entrez-Gene
Orthologous residues
SIRT1 (human): S540‑p, SIRT1 (mouse): S532‑p, SIRT1 (rat): S534‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  ES (stem), HEK293T (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Downstream Regulation
Effect of modification (function):  acetylation, enzymatic activity, induced
Effect of modification (process):  cell cycle regulation

T544-p - SIRT1 (human)
Modsite: DsssPERtsPPDsSV SwissProt Entrez-Gene
Orthologous residues
SIRT1 (human): T544‑p, SIRT1 (mouse): T536‑p, SIRT1 (rat): T538‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  ES (stem)
Cellular systems studied:  cell lines
Species studied:  human

T719-p - SIRT1 (human)
Modsite: AGGAGFGtDGDDQEA SwissProt Entrez-Gene
Orthologous residues
SIRT1 (human): T719‑p, SIRT1 (mouse): A709‑p, SIRT1 (rat): A710‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  ES (stem)
Cellular systems studied:  cell lines
Species studied:  human

S747-p - SIRT1 (human)
Modsite: MNYPSNKs_______ SwissProt Entrez-Gene
Orthologous residues
SIRT1 (human): S747‑p, SIRT1 (mouse): S737‑p, SIRT1 (rat): S738‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  ES (stem)
Cellular systems studied:  cell lines
Species studied:  human