Curated Information
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Home > Curated Information Page > PubMed Id: 19095646
Neppl RL, et al. (2009) Thromboxane A2-induced bi-directional regulation of cerebral arterial tone. J Biol Chem 284, 6348-60 19095646
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S20-p - MRLC2 (rat)
Modsite: KRPQRAtsNVFAMFD SwissProt Entrez-Gene
Orthologous residues
MRLC2 (human): S20‑p, MRLC2 (mouse): S20‑p, MRLC2 (rat): S20‑p, MRLC2 (cow): S20‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  cerebral artery
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
U46619 increase
cGMP_analog U46619 inhibit treatment-induced increase
cGMP_analog decrease

S696-p - MYPT1 (rat)
Modsite: QARQSRRstQGVTLT SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): S695‑p, MYPT1 iso2 (human): S695‑p, MYPT1 iso3 (human): S639‑p, MYPT1 iso4 (human): S636‑p, MYPT1 (mouse): S693‑p, MYPT1 (rat): S696‑p, MYPT1 (pig): S695‑p, MYPT1 (chicken): S694‑p, MYPT1 (cow): S695‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  cerebral artery
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
U46619 increase
cGMP_analog U46619 no effect upon treatment-induced increase
cGMP_analog, Triton_X-100 increase
L-NAME decrease
L-NAME U46619 inhibit treatment-induced increase

T697-p - MYPT1 (rat)
Modsite: ARQSRRstQGVTLTD SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): T696‑p, MYPT1 iso2 (human): T696‑p, MYPT1 iso3 (human): T640‑p, MYPT1 iso4 (human): T637‑p, MYPT1 (mouse): T694‑p, MYPT1 (rat): T697‑p, MYPT1 (pig): T696‑p, MYPT1 (chicken): T695‑p, MYPT1 (cow): T696‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  cerebral artery
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
U46619 increase
cGMP_analog U46619 no effect upon treatment-induced increase
cGMP_analog no change compared to control
L-NAME increase
L-NAME U46619 augment treatment-induced increase
Downstream Regulation
Effect of modification (function):  activity, inhibited

T855-p - MYPT1 (rat)
Modsite: PREKRRstGVSFWTQ SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): T853‑p, MYPT1 iso2 (human): T853‑p, MYPT1 iso3 (human): T797‑p, MYPT1 iso4 (human): T794‑p, MYPT1 (mouse): T852‑p, MYPT1 (rat): T855‑p, MYPT1 (pig): , MYPT1 (chicken): T850‑p, MYPT1 (cow): T851‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  cerebral artery
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
U46619 increase
cGMP_analog U46619 no effect upon treatment-induced increase
cGMP_analog no change compared to control
L-NAME increase
L-NAME U46619 augment treatment-induced increase
Downstream Regulation
Effect of modification (function):  activity, inhibited, intracellular localization

T38-p - PPP1R14A (rat)
Modsite: QKRHARVtVkYDRRE SwissProt Entrez-Gene
Orthologous residues
PPP1R14A (human): T38‑p, PPP1R14A iso2 (human): T38‑p, PPP1R14A (mouse): T38‑p, PPP1R14A (rat): T38‑p, PPP1R14A (pig): T38‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  cerebral artery
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
U46619 no change compared to control
cGMP_analog U46619 no change compared to control

S154-p - VASP (rat)
Modsite: EHLERRVsNAGGPPA SwissProt Entrez-Gene
Orthologous residues
VASP (human): S157‑p, VASP (mouse): S153‑p, VASP (rat): S154‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  cerebral artery
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
L-NAME decrease

S236-p - VASP (rat)
Modsite: GAKLRKVsKEEASGG SwissProt Entrez-Gene
Orthologous residues
VASP (human): S239‑p, VASP (mouse): S235‑p, VASP (rat): S236‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  cerebral artery
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cGMP_analog increase
L-NAME decrease