Boo YC, et al. (2002) Shear stress stimulates phosphorylation of endothelial nitric-oxide synthase at Ser1179 by Akt-independent mechanisms: role of protein kinase A. J Biol Chem 277, 3388-96 11729190

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T308-p - Akt1 (human) ▲

Modsite: kDGAtMKtFCGtPEy SwissProt Entrez-Gene Orthologous residues Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  BAEC (endothelial) Cellular systems studied:  primary cultured cells Species studied:  cow Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes fluid_shear_stress increase wortmannin fluid_shear_stress inhibit treatment-induced increase

S473-p - Akt1 (human) ▲

Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene Orthologous residues Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  BAEC (endothelial) Cellular systems studied:  primary cultured cells Species studied:  cow Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes fluid_shear_stress increase wortmannin fluid_shear_stress inhibit treatment-induced increase

S1179-p - eNOS (cow) ▲

Modsite: TSRIRtQsFsLQERH SwissProt Entrez-Gene Orthologous residues eNOS (human): S1177‑p, eNOS (mouse): S1176‑p, eNOS (rat): S1176‑p, eNOS (rabbit): S1183‑p, eNOS (pig): S1179‑p, eNOS (cow): S1179‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  BAEC (endothelial) Cellular systems studied:  primary cultured cells Species studied:  cow Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes fluid_shear_stress increase H-89 fluid_shear_stress inhibit treatment-induced increase wortmannin fluid_shear_stress inhibit treatment-induced increase cAMP_analog increase H-89 cAMP_analog inhibit treatment-induced increase VEGF increase