Curated Information
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Home > Curated Information Page > PubMed Id: 27484798
Dema A, et al. (2016) The A-Kinase Anchoring Protein (AKAP) Glycogen Synthase Kinase 3β Interaction Protein (GSKIP) Regulates β-Catenin through Its Interactions with Both Protein Kinase A (PKA) and GSK3β. J Biol Chem 291, 19618-30 27484798
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S33-p - CTNNB1 (human)
Modsite: QQQsyLDsGIHsGAT SwissProt Entrez-Gene
Orthologous residues
CTNNB1 (human): S33‑p, CTNNB1 (mouse): S33‑p, CTNNB1 (rat): S33‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
GSKIP (human) no change compared to control siGSKIP no change
MG132 increase
MG132 GSKIP (human) increase siGSKIP

S37-p - CTNNB1 (human)
Modsite: yLDsGIHsGATtTAP SwissProt Entrez-Gene
Orthologous residues
CTNNB1 (human): S37‑p, CTNNB1 (mouse): S37‑p, CTNNB1 (rat): S37‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
GSKIP (human) no change compared to control siGSKIP no change
MG132 increase
MG132 GSKIP (human) increase siGSKIP

T41-p - CTNNB1 (human)
Modsite: GIHsGATtTAPsLsG SwissProt Entrez-Gene
Orthologous residues
CTNNB1 (human): T41‑p, CTNNB1 (mouse): T41‑p, CTNNB1 (rat): T41‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
GSKIP (human) no change compared to control siGSKIP no change
MG132 increase
MG132 GSKIP (human) increase siGSKIP

S675-p - CTNNB1 (human)
Modsite: QDykKRLsVELtsSL SwissProt Entrez-Gene
Orthologous residues
CTNNB1 (human): S675‑p, CTNNB1 (mouse): S675‑p, CTNNB1 (rat): S675‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Wnt increase
PKI Wnt inhibit treatment-induced increase
H-89 Wnt inhibit treatment-induced increase
GSKIP (human) decrease GSKIP siRNA increase