Murga-Zamalloa CA, et al. (2017) NPM-ALK phosphorylates WASp Y102 and contributes to oncogenesis of anaplastic large cell lymphoma. Oncogene 36, 2085-2094 27694894

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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Y83-p - WASP (human) ▲

Modsite: kDNPQkSyFIRLYGL SwissProt Entrez-Gene Orthologous residues WASP (human): Y83‑p, WASP (mouse): Y85‑p, WASP (rat): Y83‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  lymphoma, anaplastic large cell lymphoma, T cell lymphoma Relevant cell lines - cell types - tissues:  HEK293T (epithelial), Karpas-299 (T lymphocyte), SUP-M2 (T lymphocyte) Cellular systems studied:  cell lines Species studied:  human

Y102-p - WASP (human) ▲

Modsite: LLWEQELySQLVYST SwissProt Entrez-Gene Orthologous residues WASP (human): Y102‑p, WASP (mouse): Y104‑p, WASP (rat): Y102‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  lymphoma, anaplastic large cell lymphoma, T cell lymphoma Relevant cell lines - cell types - tissues:  HEK293T (epithelial), Karpas-299 (T lymphocyte), SUP-M2 (T lymphocyte) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE NPM-ALK (human) Downstream Regulation Effect of modification (function):  molecular association, regulation, protein degradation Effect of modification (process):  carcinogenesis, induced, cell growth, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays WIPF1 (human) Disrupts pull-down assay

Y291-p - WASP (human) ▲

Modsite: AEtsKLIyDFIEDQG SwissProt Entrez-Gene Orthologous residues WASP (human): Y291‑p, WASP (mouse): Y293‑p, WASP (rat): Y291‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  lymphoma, anaplastic large cell lymphoma, T cell lymphoma Relevant cell lines - cell types - tissues:  HEK293T (epithelial), Karpas-299 (T lymphocyte), SUP-M2 (T lymphocyte) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE NPM-ALK (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE NPM-ALK (human) siRNA inhibition of enzyme, transfection of inactive enzyme, transfection of wild-type enzyme, pharmacological inhibitor of upstream enzyme, phospho-antibody