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Home > Curated Information Page > PubMed Id: 27603133
Chiu HC, et al. (2016) Suppression of Vimentin Phosphorylation by the Avian Reovirus p17 through Inhibition of CDK1 and Plk1 Impacting the G2/M Phase of the Cell Cycle. PLoS One 11, e0162356 27603133
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S1981-p - ATM (human)
Modsite: sLAFEEGsQSTtIss SwissProt Entrez-Gene
Orthologous residues
ATM (human): S1981‑p, ATM (mouse): S1987‑p, ATM (rat): S1988‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  DF1 (fibroblast), Vero (epithelial)
Cellular systems studied:  cell lines
Species studied:  chicken, green monkey
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
increase avian reovirus (ARV) infection, P17 transfection

S216-p - CDC25C (human)
Modsite: sGLyRsPsMPENLNR SwissProt Entrez-Gene
Orthologous residues
CDC25C (human): S216‑p, CDC25C iso3 (human): S173‑p, CDC25C (mouse): , CDC25C (frog): S287‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  DF1 (fibroblast), Vero (epithelial)
Cellular systems studied:  cell lines
Species studied:  chicken, green monkey
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
decrease ARV infection, P17 transfection
MG132 increase in ARV-infected and p17-transfected cells

T14-p - CDK1 (human)
Modsite: IEkIGEGtyGVVykG SwissProt Entrez-Gene
Orthologous residues
CDK1 (human): T14‑p, CDK1 iso2 (human): T14‑p, CDK1 (mouse): T14‑p, CDK1 (rat): T14‑p, CDK1 (chicken): T14‑p, CDK1 (fruit fly): T14‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  DF1 (fibroblast), Vero (epithelial)
Cellular systems studied:  cell lines
Species studied:  chicken, green monkey
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
increase avian reovirus (ARV) infection, P17 transfection

Y15-p - CDK1 (human)
Modsite: EkIGEGtyGVVykGR SwissProt Entrez-Gene
Orthologous residues
CDK1 (human): Y15‑p, CDK1 iso2 (human): Y15‑p, CDK1 (mouse): Y15‑p, CDK1 (rat): Y15‑p, CDK1 (chicken): Y15‑p, CDK1 (fruit fly): Y15‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  DF1 (fibroblast), Vero (epithelial)
Cellular systems studied:  cell lines
Species studied:  chicken, green monkey
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
increase avian reovirus (ARV) infection, P17 transfection

T495-p - Myt1 (human)
Modsite: LLSLFEDtLDPT___ SwissProt Entrez-Gene
Orthologous residues
Myt1 (human): T495‑p, Myt1 (mouse): S486‑p, Myt1 (rat): S486‑p, Myt1 (starfish): T545‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  DF1 (fibroblast), Vero (epithelial)
Cellular systems studied:  cell lines
Species studied:  chicken, green monkey

T145-p - p21Cip1 (human)
Modsite: QGRkRRQtsMTDFyH SwissProt Entrez-Gene
Orthologous residues
p21Cip1 (human): T145‑p, p21Cip1 (mouse): T140‑p, p21Cip1 (dog): T113‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  DF1 (fibroblast), Vero (epithelial)
Cellular systems studied:  cell lines
Species studied:  chicken, green monkey

S15-p - p53 (human)
Modsite: PsVEPPLsQEtFsDL SwissProt Entrez-Gene
Orthologous residues
p53 (human): S15‑p, p53 iso2 (human): S15‑p, p53 iso4 (human): , p53 (mouse): S18‑p, p53 iso2 (mouse): S18‑p, p53 (rat): S15‑p, p53 (rabbit): S15‑p, p53 (green monkey): S15‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  DF1 (fibroblast), Vero (epithelial)
Cellular systems studied:  cell lines
Species studied:  chicken, green monkey
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
increase avian reovirus (ARV) infection, P17 transfection

T210-p - PLK1 (human)
Modsite: YDGERKktLCGtPNy SwissProt Entrez-Gene
Orthologous residues
PLK1 (human): T210‑p, PLK1 (mouse): T210‑p, PLK1 (rat): T210‑p, PLK1 (frog): T201‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  DF1 (fibroblast), Vero (epithelial)
Cellular systems studied:  cell lines
Species studied:  chicken, green monkey
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
decrease ARV infection, P17 transfection

S56-p - Vimentin (human)
Modsite: srsLyAssPGGVyAt SwissProt Entrez-Gene
Orthologous residues
Vimentin (human): S56‑p, Vimentin (mouse): S56‑p, Vimentin (rat): S56‑p, Vimentin (rabbit): S57‑p, Vimentin (hamster): S55‑p, Vimentin (cow): S56‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  DF1 (fibroblast), Vero (epithelial)
Cellular systems studied:  cell lines
Species studied:  chicken, green monkey
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
decrease ARV infection, P17 transfection
p53 (human) decrease upregulation of p53 by p17
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CDK1 (human) Induces co-immunoprecipitation
Comments:  Avian Reovirus p17 binds to CDK1and vimentin both of which inhibit the CDK1-vimentin enzyme-substrate reaction

S83-p - Vimentin (human)
Modsite: GVRLLQDsVdFsLAd SwissProt Entrez-Gene
Orthologous residues
Vimentin (human): S83‑p, Vimentin (mouse): S83‑p, Vimentin (rat): S83‑p, Vimentin (rabbit): S83‑p, Vimentin (hamster): S82‑p, Vimentin (cow): S83‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  DF1 (fibroblast), Vero (epithelial)
Cellular systems studied:  cell lines
Species studied:  chicken, green monkey
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CDK1 (human) Induces co-immunoprecipitation
Comments:  Avian Reovirus p17 binds to CDK1and vimentin both of which inhibit the CDK1-vimentin enzyme-substrate reaction