Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 27626683
Yoshioka Y, et al. (2016) SMYD3-mediated lysine methylation in the PH domain is critical for activation of AKT1. Oncotarget 7, 75023-75037 27626683
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

K14-m1 - Akt1 (human)
Modsite: VkEGWLHkRGEYIkT SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): K14‑m1, Akt1 iso2 (human): , Akt1 (mouse): K14‑m1, Akt1 (rat): K14‑m1, Akt1 (fruit fly): K115‑m1, Akt1 (cow): K14‑m1
Characterization
Methods used to characterize site in vivo modification-specific antibody, mutation of modification site
Disease tissue studied:  breast cancer, colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MDA-MB-231 (breast cell), SW480 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
METHYLTRANSFERASE SMYD3 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
METHYLTRANSFERASE SMYD3 (human) genetic knockout/knockin of upstream enzyme, transfection of wild-type enzyme, transfection of inactive enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BCI-121 decrease
Downstream Regulation
Effect of modification (function):  activity, induced, intracellular localization, phosphorylation
Effect of modification (process):  cell growth, induced

T308-p - Akt1 (human)
Modsite: kDGAtMKtFCGtPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BCI-121 decrease