Curated Information
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Home > Curated Information Page > PubMed Id: 12417722
Law BK, et al. (2002) Rapamycin potentiates transforming growth factor beta-induced growth arrest in nontransformed, oncogene-transformed, and human cancer cells. Mol Cell Biol 22, 8184-98 12417722
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T160-p - CDK2 (human)
Modsite: GVPVRtytHEVVtLW SwissProt Entrez-Gene
Orthologous residues
CDK2 (human): T160‑p, CDK2 iso2 (human): T160‑p, CDK2 (mouse): T160‑p, CDK2 (rat): T160‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  prostate cancer
Relevant cell lines - cell types - tissues:  DU 145 (prostate cell)
Cellular systems studied:  cell lines
Species studied:  human

S243-p - Rb (mouse)
Modsite: AAIPINGsPRtPRRG SwissProt Entrez-Gene
Orthologous residues
Rb (human): S249‑p, Rb (mouse): S243‑p, Rb (rat): S242‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  NMuMG (epithelial)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TGF-beta decrease
rapamycin TGF-beta augment treatment-induced decrease
rapamycin no change compared to control

T246-p - Rb (mouse)
Modsite: PINGsPRtPRRGQNR SwissProt Entrez-Gene
Orthologous residues
Rb (human): T252‑p, Rb (mouse): T246‑p, Rb (rat): T245‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  NMuMG (epithelial)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TGF-beta decrease
rapamycin TGF-beta augment treatment-induced decrease
rapamycin no change compared to control

T814-p - Rb (mouse)
Modsite: KIsEGLPtPtKMtPR SwissProt Entrez-Gene
Orthologous residues
Rb (human): T821‑p, Rb (mouse): T814‑p, Rb (rat): T813‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  NMuMG (epithelial)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TGF-beta decrease
rapamycin TGF-beta augment treatment-induced decrease
rapamycin no change compared to control