Curated Information
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Home > Curated Information Page > PubMed Id: 12807916
Kristof AS, Marks-Konczalik J, Billings E, Moss J (2003) Stimulation of signal transducer and activator of transcription-1 (STAT1)-dependent gene transcription by lipopolysaccharide and interferon-gamma is regulated by mammalian target of rapamycin. J Biol Chem 278, 33637-44 12807916
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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S2448-p - mTOR (human)
Modsite: RsRtRtDsysAGQsV SwissProt Entrez-Gene
Orthologous residues
mTOR (human): S2448‑p, mTOR (mouse): S2448‑p, mTOR (rat): S2448‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Disease tissue studied:  lung cancer
Relevant cell lines - cell types - tissues:  A549 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IFN-gamma, LPS increase
wortmannin IFN-gamma, LPS no effect upon treatment-induced increase
wortmannin no change compared to control
LY294002 IFN-gamma, LPS no effect upon treatment-induced increase
LY294002 no change compared to control
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S2481-p - mTOR (human)
Modsite: tVPEsIHsFIGDGLV SwissProt Entrez-Gene
Orthologous residues
mTOR (human): S2481‑p, mTOR (mouse): S2481‑p, mTOR (rat): S2481‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Disease tissue studied:  lung cancer
Relevant cell lines - cell types - tissues:  A549 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IFN-gamma, LPS increase
wortmannin IFN-gamma, LPS no effect upon treatment-induced increase
wortmannin no change compared to control
LY294002 IFN-gamma, LPS inhibit treatment-induced increase
LY294002 decrease
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

T412-p - p70S6K (human)
Modsite: NQVFLGFtyVAPsVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Disease tissue studied:  lung cancer
Relevant cell lines - cell types - tissues:  A549 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IFN-gamma, LPS increase
wortmannin IFN-gamma, LPS inhibit treatment-induced increase
LY294002 decrease
wortmannin decrease
LY294002 IFN-gamma, LPS augment treatment-induced increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

Y701-p - STAT1 (human)
Modsite: DGPkGtGyIktELIs SwissProt Entrez-Gene
Orthologous residues
STAT1 (human): Y701‑p, STAT1 iso2 (human): Y701‑p, STAT1 (mouse): Y701‑p, STAT1 (rat): Y701‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Disease tissue studied:  lung cancer
Relevant cell lines - cell types - tissues:  A549 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IFN-gamma, LPS increase
wortmannin IFN-gamma, LPS no effect upon treatment-induced increase
wortmannin no change compared to control
LY294002 IFN-gamma, LPS no effect upon treatment-induced increase
LY294002 no change compared to control
Downstream Regulation
Effect of modification (function):  activity, induced

S727-p - STAT1 (human)
Modsite: TDNLLPMsPEEFDEV SwissProt Entrez-Gene
Orthologous residues
STAT1 (human): S727‑p, STAT1 iso2 (human): , STAT1 (mouse): S727‑p, STAT1 (rat): S727‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Disease tissue studied:  lung cancer
Relevant cell lines - cell types - tissues:  A549 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase
IFN-gamma increase
IFN-gamma, LPS increase
LY294002 decrease
LY294002 IFN-gamma, LPS inhibit treatment-induced increase
wortmannin IFN-gamma, LPS no effect upon treatment-induced increase
wortmannin no change compared to control
Downstream Regulation
Effect of modification (function):  activity, induced