Park S, et al. (2016) Interplay between Leucine-Rich Repeat Kinase 2 (LRRK2) and p62/SQSTM-1 in Selective Autophagy. PLoS One 11, e0163029 27631370

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S348-p - SQSTM1 (rat) ▲

Modsite: SSKEVDPsTGELQsL SwissProt Entrez-Gene Orthologous residues SQSTM1 (human): S349‑p, SQSTM1 iso2 (human): S265‑p, SQSTM1 (mouse): S351‑p, SQSTM1 iso2 (mouse): S351‑p, SQSTM1 (rat): S348‑p, SQSTM1 iso2 (rat): S321‑p, SQSTM1 iso3 (rat): ‑ Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'neuron, cortical'-brain, HEK293T (epithelial) Cellular systems studied:  cell lines, primary cells Species studied:  human, rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes LRRK2 (human) decrease LRRK2 siRNA increase Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays KEAP1 (rat) Induces co-immunoprecipitation Comments:  reduced by LRRK2 overexpression

S402-p - SQSTM1 (rat) ▲

Modsite: ESLSQMLsMGFSDEG SwissProt Entrez-Gene Orthologous residues SQSTM1 (human): S403‑p, SQSTM1 iso2 (human): S319‑p, SQSTM1 (mouse): S405‑p, SQSTM1 iso2 (mouse): S367‑p, SQSTM1 (rat): S402‑p, SQSTM1 iso2 (rat): S375‑p, SQSTM1 iso3 (rat): ‑ Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'neuron, cortical'-brain, HEK293T (epithelial) Cellular systems studied:  cell lines, primary cells Species studied:  human, rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes LRRK2 (human) decrease LRRK2 siRNA increase Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  autophagy, altered Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays LRRK2 (rat) Induces protein degradation co-immunoprecipitation