Gao W, et al. (2016) Site-specific phosphorylation and microtubule dynamics control Pyrin inflammasome activation. Proc Natl Acad Sci U S A 113, E4857-66 27482109

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S205-p - pyrin (mouse) ▲

Modsite: AQLRRNVsSAGRLQG SwissProt Entrez-Gene Orthologous residues pyrin (human): S208‑p, pyrin (mouse): S205‑p, pyrin (rat): Q204‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), macrophage-bone marrow Cellular systems studied:  cell lines, primary cells Species studied:  human, mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes Clostridium_toxin_B decrease C. difficile Cytotoxin TcdA decrease Downstream Regulation Effect of modification (function):  activity, inhibited, molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays 14-3-3 epsilon (mouse) Induces co-immunoprecipitation Comments:  dephosphorylation triggers 14-3-3 dissociation, activation, formation of an oligomeric Pyrin inflammasomecomplex

S241-p - pyrin (mouse) ▲

Modsite: SGKKRPRsLEITTYS SwissProt Entrez-Gene Orthologous residues pyrin (human): S242‑p, pyrin (mouse): S241‑p, pyrin (rat): V238‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), macrophage-bone marrow Cellular systems studied:  cell lines, primary cells Species studied:  human, mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes Clostridium_toxin_B decrease C. difficile Cytotoxin TcdA decrease Downstream Regulation Effect of modification (function):  activity, inhibited, molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays 14-3-3 epsilon (mouse) Induces co-immunoprecipitation Comments:  dephosphorylation triggers 14-3-3 dissociation, activation, formation of an oligomeric Pyrin inflammasomecomplex