Miranda F, et al. (2016) Salt-Inducible Kinase 2 Couples Ovarian Cancer Cell Metabolism with Survival at the Adipocyte-Rich Metastatic Niche. Cancer Cell 30, 273-89 27478041

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S154-p - PIK3R1 (human) ▲

Modsite: stLYRtQsSSNLAEL SwissProt Entrez-Gene Orthologous residues PIK3R1 (human): S154‑p, PIK3R1 iso2 (human): ‑, PIK3R1 iso4 (human): S154‑p, PIK3R1 (mouse): S154‑p, PIK3R1 (rat): S154‑p, PIK3R1 (cow): S154‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  ovarian cancer Relevant cell lines - cell types - tissues:  adipocyte, HEK293T (epithelial), SKOV-3 (ovarian) Cellular systems studied:  cell lines, primary cells Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE QIK (human)

S541-p - PIK3R1 (human) ▲

Modsite: RISEIIDsRRRLEED SwissProt Entrez-Gene Orthologous residues PIK3R1 (human): S541‑p, PIK3R1 iso2 (human): S271‑p, PIK3R1 iso4 (human): S541‑p, PIK3R1 (mouse): S541‑p, PIK3R1 (rat): S541‑p, PIK3R1 (cow): S541‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  ovarian cancer Relevant cell lines - cell types - tissues:  adipocyte, HEK293T (epithelial), SKOV-3 (ovarian) Cellular systems studied:  cell lines, primary cells Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE QIK (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE QIK (human) siRNA inhibition of enzyme

S90-p - QIK (human) ▲

Modsite: YQVMETKsMLYLVTE SwissProt Entrez-Gene Orthologous residues QIK (human): S90‑p, QIK (mouse): S90‑p, QIK (rat): S90‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  ovarian cancer Relevant cell lines - cell types - tissues:  adipocyte, HEK293T (epithelial), SKOV-3 (ovarian) Cellular systems studied:  cell lines, primary cells Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE QIK (human) Comments:  autophosphoryation sites Downstream Regulation Effect of modification (function):  enzymatic activity, induced

T175-p - QIK (human) ▲

Modsite: kSGELLAtWCGSPPY SwissProt Entrez-Gene Orthologous residues QIK (human): T175‑p, QIK (mouse): T175‑p, QIK (rat): T175‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  ovarian cancer Relevant cell lines - cell types - tissues:  adipocyte, HEK293T (epithelial), SKOV-3 (ovarian) Cellular systems studied:  cell lines, primary cells Species studied:  human Downstream Regulation Effect of modification (function):  enzymatic activity, induced

S343-p - QIK (human) ▲

Modsite: RLKSHRssFPVEQRL SwissProt Entrez-Gene Orthologous residues QIK (human): S343‑p, QIK (mouse): S343‑p, QIK (rat): S343‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  ovarian cancer Relevant cell lines - cell types - tissues:  adipocyte, HEK293T (epithelial), SKOV-3 (ovarian) Cellular systems studied:  cell lines, primary cells Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE QIK (human) Comments:  autophosphoryation sites Downstream Regulation Effect of modification (function):  enzymatic activity, induced

S358-p - QIK (human) ▲

Modsite: DGRQRRPstIAEQTV SwissProt Entrez-Gene Orthologous residues QIK (human): S358‑p, QIK (mouse): S358‑p, QIK (rat): S358‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  ovarian cancer Relevant cell lines - cell types - tissues:  adipocyte, HEK293T (epithelial), SKOV-3 (ovarian) Cellular systems studied:  cell lines, primary cells Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE QIK (human) Comments:  autophosphoryation sites Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes dasatinib decrease HG9-91-01 decrease BAPTA-AM decrease H-89 no change compared to control rapamycin increase