Shah OJ, Ghosh S, Hunter T (2003) Mitotic regulation of ribosomal S6 kinase 1 involves Ser/Thr, Pro phosphorylation of consensus and non-consensus sites by Cdc2. J Biol Chem 278, 16433-42 12586835

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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S394-p - p70S6K (human) ▲

Modsite: TRQtPVDsPDDStLS SwissProt Entrez-Gene Orthologous residues p70S6K (human): S394‑p, p70S6K iso2 (human): S371‑p, p70S6K (mouse): S394‑p, p70S6K (rat): S394‑p, p70S6K iso2 (rat): S371‑p, p70S6K (fruit fly): S380‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  FT210 (breast cell), HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE CDK1 (human) genetic knockout/knockin of upstream enzyme, pharmacological inhibitor of upstream enzyme

T412-p - p70S6K (human) ▲

Modsite: NQVFLGFtyVAPsVL SwissProt Entrez-Gene Orthologous residues p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  FT210 (breast cell), HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes nocodazole decrease

S434-p - p70S6K (human) ▲

Modsite: sFEPKIRsPRRFIGs SwissProt Entrez-Gene Orthologous residues p70S6K (human): S434‑p, p70S6K iso2 (human): S411‑p, p70S6K (mouse): S434‑p, p70S6K (rat): S434‑p, p70S6K iso2 (rat): S411‑p, p70S6K (fruit fly): S418‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  FT210 (breast cell), HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE CDK1 (human) genetic knockout/knockin of upstream enzyme, pharmacological inhibitor of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes nocodazole increase vanadate nocodazole inhibit treatment-induced increase purvalanol nocodazole inhibit treatment-induced increase seliciclib nocodazole inhibit treatment-induced increase rapamycin nocodazole no effect upon treatment-induced increase U0126 nocodazole no effect upon treatment-induced increase PD98059 nocodazole no effect upon treatment-induced increase SB202190 nocodazole no effect upon treatment-induced increase wortmannin nocodazole no effect upon treatment-induced increase

T444-p - p70S6K (human) ▲

Modsite: RFIGsPRtPVsPVkF SwissProt Entrez-Gene Orthologous residues p70S6K (human): T444‑p, p70S6K iso2 (human): T421‑p, p70S6K (mouse): T444‑p, p70S6K (rat): T444‑p, p70S6K iso2 (rat): T421‑p, p70S6K (fruit fly): S429‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  FT210 (breast cell), HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes nocodazole increase vanadate nocodazole inhibit treatment-induced increase purvalanol nocodazole inhibit treatment-induced increase seliciclib nocodazole inhibit treatment-induced increase rapamycin nocodazole no effect upon treatment-induced increase U0126 nocodazole no effect upon treatment-induced increase PD98059 nocodazole no effect upon treatment-induced increase SB202190 nocodazole no effect upon treatment-induced increase wortmannin nocodazole no effect upon treatment-induced increase

S447-p - p70S6K (human) ▲

Modsite: GsPRtPVsPVkFsPG SwissProt Entrez-Gene Orthologous residues p70S6K (human): S447‑p, p70S6K iso2 (human): S424‑p, p70S6K (mouse): S447‑p, p70S6K (rat): S447‑p, p70S6K iso2 (rat): S424‑p, p70S6K (fruit fly): S430‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  FT210 (breast cell), HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE CDK1 (human) genetic knockout/knockin of upstream enzyme, pharmacological inhibitor of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes nocodazole increase vanadate nocodazole inhibit treatment-induced increase purvalanol nocodazole inhibit treatment-induced increase seliciclib nocodazole inhibit treatment-induced increase rapamycin nocodazole no effect upon treatment-induced increase U0126 nocodazole no effect upon treatment-induced increase PD98059 nocodazole no effect upon treatment-induced increase SB202190 nocodazole no effect upon treatment-induced increase wortmannin nocodazole no effect upon treatment-induced increase

S240-p - S6 (human) ▲

Modsite: RLssLRAstsKsEss SwissProt Entrez-Gene Orthologous residues S6 (human): S240‑p, S6 (mouse): S240‑p, S6 (rat): S240‑p, S6 (fruit fly): S239‑p, S6 (cow): S240‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  FT210 (breast cell), HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes nocodazole decrease

S244-p - S6 (human) ▲

Modsite: LRAstsKsEssQK__ SwissProt Entrez-Gene Orthologous residues S6 (human): S244‑p, S6 (mouse): S244‑p, S6 (rat): S244‑p, S6 (fruit fly): V243‑p, S6 (cow): S244‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  FT210 (breast cell), HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes nocodazole decrease