Jideama NM, et al. (1996) Phosphorylation specificities of protein kinase C isozymes for bovine cardiac troponin I and troponin T and sites within these proteins and regulation of myofilament properties. J Biol Chem 271, 23277-83 8798526

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S23-p - TNNI3 (rat) ▲

Modsite: PAPVRRRssANYRAY SwissProt Entrez-Gene Orthologous residues TNNI3 (human): S23‑p, TNNI3 (mouse): S23‑p, TNNI3 (rat): S23‑p, TNNI3 (rabbit): S22‑p, TNNI3 (dog): S23‑p, TNNI3 (cow): S24‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, phosphopeptide mapping Relevant cell lines - cell types - tissues:  myocyte-heart Cellular systems studied:  primary cultured cells Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase

S24-p - TNNI3 (rat) ▲

Modsite: APVRRRssANYRAYA SwissProt Entrez-Gene Orthologous residues TNNI3 (human): S24‑p, TNNI3 (mouse): S24‑p, TNNI3 (rat): S24‑p, TNNI3 (rabbit): S23‑p, TNNI3 (dog): S24‑p, TNNI3 (cow): S25‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, phosphopeptide mapping Relevant cell lines - cell types - tissues:  myocyte-heart Cellular systems studied:  primary cultured cells Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase

S43-p - TNNI3 (rat) ▲

Modsite: AKKKSKIsAsRKLQL SwissProt Entrez-Gene Orthologous residues TNNI3 (human): S42‑p, TNNI3 (mouse): S43‑p, TNNI3 (rat): S43‑p, TNNI3 (rabbit): S42‑p, TNNI3 (dog): S43‑p, TNNI3 (cow): S44‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, phosphopeptide mapping Relevant cell lines - cell types - tissues:  myocyte-heart Cellular systems studied:  primary cultured cells Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase

S45-p - TNNI3 (rat) ▲

Modsite: KKSKIsAsRKLQLkT SwissProt Entrez-Gene Orthologous residues TNNI3 (human): S44‑p, TNNI3 (mouse): S45‑p, TNNI3 (rat): S45‑p, TNNI3 (rabbit): S44‑p, TNNI3 (dog): S45‑p, TNNI3 (cow): S46‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, phosphopeptide mapping Relevant cell lines - cell types - tissues:  myocyte-heart Cellular systems studied:  primary cultured cells Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase

S78-p - TNNI3 (rat) ▲

Modsite: GEKGRVLsTRCQPLV SwissProt Entrez-Gene Orthologous residues TNNI3 (human): S77‑p, TNNI3 (mouse): R78‑p, TNNI3 (rat): S78‑p, TNNI3 (rabbit): S77‑p, TNNI3 (dog): S78‑p, TNNI3 (cow): S79‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, phosphopeptide mapping Relevant cell lines - cell types - tissues:  myocyte-heart Cellular systems studied:  primary cultured cells Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase

T144-p - TNNI3 (rat) ▲

Modsite: RGKFkRPtLRRVRIs SwissProt Entrez-Gene Orthologous residues TNNI3 (human): T143‑p, TNNI3 (mouse): T144‑p, TNNI3 (rat): T144‑p, TNNI3 (rabbit): T143‑p, TNNI3 (dog): T144‑p, TNNI3 (cow): T145‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, phosphopeptide mapping Relevant cell lines - cell types - tissues:  myocyte-heart Cellular systems studied:  primary cultured cells Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase

T205-p - TNNT2 (rat) ▲

Modsite: GYIQkAQtERKsGKR SwissProt Entrez-Gene Orthologous residues TNNT2 (human): T204‑p, TNNT2 (mouse): T207‑p, TNNT2 iso2 (mouse): T194‑p, TNNT2 (rat): T205‑p, TNNT2 iso2 (rat): T195‑p, TNNT2 (rabbit): T207‑p, TNNT2 (cow): T191‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, phosphopeptide mapping Relevant cell lines - cell types - tissues:  myocyte-heart Cellular systems studied:  primary cultured cells Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase

S209-p - TNNT2 (rat) ▲

Modsite: kAQtERKsGKRQtER SwissProt Entrez-Gene Orthologous residues TNNT2 (human): S208‑p, TNNT2 (mouse): S211‑p, TNNT2 iso2 (mouse): S198‑p, TNNT2 (rat): S209‑p, TNNT2 iso2 (rat): S199‑p, TNNT2 (rabbit): S211‑p, TNNT2 (cow): S195‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, phosphopeptide mapping Relevant cell lines - cell types - tissues:  myocyte-heart Cellular systems studied:  primary cultured cells Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase

T214-p - TNNT2 (rat) ▲

Modsite: RKsGKRQtEREKKKK SwissProt Entrez-Gene Orthologous residues TNNT2 (human): T213‑p, TNNT2 (mouse): T216‑p, TNNT2 iso2 (mouse): T203‑p, TNNT2 (rat): T214‑p, TNNT2 iso2 (rat): T204‑p, TNNT2 (rabbit): T216‑p, TNNT2 (cow): T200‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, phosphopeptide mapping Relevant cell lines - cell types - tissues:  myocyte-heart Cellular systems studied:  primary cultured cells Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase

T295-p - TNNT2 (rat) ▲

Modsite: TRGKAKVtGRWK___ SwissProt Entrez-Gene Orthologous residues TNNT2 (human): T294‑p, TNNT2 (mouse): T297‑p, TNNT2 iso2 (mouse): T284‑p, TNNT2 (rat): T295‑p, TNNT2 iso2 (rat): T285‑p, TNNT2 (rabbit): T297‑p, TNNT2 (cow): T281‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, phosphopeptide mapping Relevant cell lines - cell types - tissues:  myocyte-heart Cellular systems studied:  primary cultured cells Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase

S24-p - TNNI3 (cow) ▲
Modsite: PPPVRRRssANYRAY SwissProt Entrez-Gene Orthologous residues TNNI3 (human): S23‑p, TNNI3 (mouse): S23‑p, TNNI3 (rat): S23‑p, TNNI3 (rabbit): S22‑p, TNNI3 (dog): S23‑p, TNNI3 (cow): S24‑p Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKCD (human) KINASE PKCA (human) KINASE PKACA (human)

S25-p - TNNI3 (cow) ▲
Modsite: PPVRRRssANYRAYA SwissProt Entrez-Gene Orthologous residues TNNI3 (human): S24‑p, TNNI3 (mouse): S24‑p, TNNI3 (rat): S24‑p, TNNI3 (rabbit): S23‑p, TNNI3 (dog): S24‑p, TNNI3 (cow): S25‑p Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKCA (human) KINASE PKACA (human) KINASE PKCD (human)

S44-p - TNNI3 (cow) ▲
Modsite: AKKKSKIsAsRKLQL SwissProt Entrez-Gene Orthologous residues TNNI3 (human): S42‑p, TNNI3 (mouse): S43‑p, TNNI3 (rat): S43‑p, TNNI3 (rabbit): S42‑p, TNNI3 (dog): S43‑p, TNNI3 (cow): S44‑p Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKCA (human) KINASE PKACA (human) KINASE PKCE (human) KINASE PKCZ (human) KINASE PKCD (human)

S46-p - TNNI3 (cow) ▲
Modsite: KKSKIsAsRKLQLKT SwissProt Entrez-Gene Orthologous residues TNNI3 (human): S44‑p, TNNI3 (mouse): S45‑p, TNNI3 (rat): S45‑p, TNNI3 (rabbit): S44‑p, TNNI3 (dog): S45‑p, TNNI3 (cow): S46‑p Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKCZ (human) KINASE PKCE (human) KINASE PKCD (human) KINASE PKCA (human) KINASE PKACA (human)

S79-p - TNNI3 (cow) ▲
Modsite: GEKGRALsTRCQPLE SwissProt Entrez-Gene Orthologous residues TNNI3 (human): S77‑p, TNNI3 (mouse): R78‑p, TNNI3 (rat): S78‑p, TNNI3 (rabbit): S77‑p, TNNI3 (dog): S78‑p, TNNI3 (cow): S79‑p Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKCD (human) KINASE PKCA (human) KINASE PKCE (human)

T145-p - TNNI3 (cow) ▲
Modsite: RGKFKRPtLRRVRIS SwissProt Entrez-Gene Orthologous residues TNNI3 (human): T143‑p, TNNI3 (mouse): T144‑p, TNNI3 (rat): T144‑p, TNNI3 (rabbit): T143‑p, TNNI3 (dog): T144‑p, TNNI3 (cow): T145‑p Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKCA (human) KINASE PKCD (human) KINASE PKCE (human)

T191-p - TNNT2 (cow) ▲
Modsite: GYIQKAQtERKsGKR SwissProt Entrez-Gene Orthologous residues TNNT2 (human): T204‑p, TNNT2 (mouse): T207‑p, TNNT2 iso2 (mouse): T194‑p, TNNT2 (rat): T205‑p, TNNT2 iso2 (rat): T195‑p, TNNT2 (rabbit): T207‑p, TNNT2 (cow): T191‑p Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKCD (human) KINASE PKCE (human)

S195-p - TNNT2 (cow) ▲
Modsite: KAQtERKsGKRQtER SwissProt Entrez-Gene Orthologous residues TNNT2 (human): S208‑p, TNNT2 (mouse): S211‑p, TNNT2 iso2 (mouse): S198‑p, TNNT2 (rat): S209‑p, TNNT2 iso2 (rat): S199‑p, TNNT2 (rabbit): S211‑p, TNNT2 (cow): S195‑p Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKCE (human) KINASE PKCD (human) KINASE PKCA (human)

T200-p - TNNT2 (cow) ▲
Modsite: RKsGKRQtEREKKKK SwissProt Entrez-Gene Orthologous residues TNNT2 (human): T213‑p, TNNT2 (mouse): T216‑p, TNNT2 iso2 (mouse): T203‑p, TNNT2 (rat): T214‑p, TNNT2 iso2 (rat): T204‑p, TNNT2 (rabbit): T216‑p, TNNT2 (cow): T200‑p Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKCA (human) KINASE PKCE (human) KINASE PKCD (human)

T281-p - TNNT2 (cow) ▲
Modsite: TRGKAKVtGRWK___ SwissProt Entrez-Gene Orthologous residues TNNT2 (human): T294‑p, TNNT2 (mouse): T297‑p, TNNT2 iso2 (mouse): T284‑p, TNNT2 (rat): T295‑p, TNNT2 iso2 (rat): T285‑p, TNNT2 (rabbit): T297‑p, TNNT2 (cow): T281‑p Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKCA (human) KINASE PKCD (human) KINASE PKCE (human)