Curated Information
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Home > Curated Information Page > PubMed Id: 12591925
Bauer PM, et al. (2003) Compensatory phosphorylation and protein-protein interactions revealed by loss of function and gain of function mutants of multiple serine phosphorylation sites in endothelial nitric-oxide synthase. J Biol Chem 278, 14841-9 12591925
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S116-p - eNOS (cow)
Modsite: RKLQTRPsPGPPPAE SwissProt Entrez-Gene
Orthologous residues
eNOS (human): S114‑p, eNOS (mouse): T113‑p, eNOS (rat): T113‑p, eNOS (rabbit): T120‑p, eNOS (pig): S116‑p, eNOS (cow): S116‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  BAEC (endothelial), COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  cow, green monkey
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
VEGF decrease
ATP decrease
Downstream Regulation
Effect of modification (function):  enzymatic activity, inhibited, molecular association, regulation, phosphorylation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Akt1 (human) Disrupts co-immunoprecipitation, electrophoretic visualization
Comments:  eNOS S115A mutation increases phosphorylation of S1178 by about 50%

S617-p - eNOS (cow)
Modsite: SYKIRFNsVSCSDPL SwissProt Entrez-Gene
Orthologous residues
eNOS (human): S615‑p, eNOS (mouse): S614‑p, eNOS (rat): S614‑p, eNOS (rabbit): S621‑p, eNOS (pig): S617‑p, eNOS (cow): S617‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  BAEC (endothelial), COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  cow, green monkey
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
VEGF increase
ATP increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation, phosphorylation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
HSP90A (human) Disrupts co-immunoprecipitation, electrophoretic visualization
Akt1 (human) Disrupts co-immunoprecipitation, electrophoretic visualization
Comments:  eNOS S616A mutant shows a reduction in phosphorylation of S115 and an increase in phosphorylation of S1178;

S635-p - eNOS (cow)
Modsite: WRRKRKEsSNTDSAG SwissProt Entrez-Gene
Orthologous residues
eNOS (human): S633‑p, eNOS (mouse): S632‑p, eNOS (rat): S632‑p, eNOS (rabbit): S639‑p, eNOS (pig): S635‑p, eNOS (cow): S635‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  BAEC (endothelial), COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  cow, green monkey
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
VEGF increase
ATP increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, phosphorylation
Comments:  2-fold higher phosphorylation of S634 in the S1179A eNOS mutant

S1179-p - eNOS (cow)
Modsite: TSRIRtQsFsLQERH SwissProt Entrez-Gene
Orthologous residues
eNOS (human): S1177‑p, eNOS (mouse): S1176‑p, eNOS (rat): S1176‑p, eNOS (rabbit): S1183‑p, eNOS (pig): S1179‑p, eNOS (cow): S1179‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  BAEC (endothelial), COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  cow, green monkey
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
VEGF increase
ATP increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, phosphorylation
Comments:  2-fold higher phosphorylation of S634 in the S1179A eNOS mutant